Transcript: Human NR_156493.2

Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT), transcript variant 2, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
PPAT (5471)
Length:
3599
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_156493.2
NBCI Gene record:
PPAT (5471)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_156493.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000075214 GCTCAAAGAATCTGGTGCAAA pLKO.1 1267 3UTR 100% 4.950 6.930 N PPAT n/a
2 TRCN0000304118 GTAGCTTCACCACCAATTAAA pLKO_005 1304 3UTR 100% 15.000 12.000 N PPAT n/a
3 TRCN0000304117 TGGTAAATGCTGCTCGATTAA pLKO_005 429 3UTR 100% 13.200 10.560 N PPAT n/a
4 TRCN0000304116 CAATACCATCTCACCTATAAT pLKO_005 1240 3UTR 100% 15.000 10.500 N PPAT n/a
5 TRCN0000304177 TGGTCACACCTCATCTATTTA pLKO_005 1666 3UTR 100% 15.000 10.500 N PPAT n/a
6 TRCN0000075217 CTTATGGAAATCGTCCCTTAT pLKO.1 654 3UTR 100% 10.800 7.560 N PPAT n/a
7 TRCN0000075215 CCCTTCGTTGTTGAAACACTT pLKO.1 374 3UTR 100% 4.950 3.465 N PPAT n/a
8 TRCN0000300653 CCCTTCGTTGTTGAAACACTT pLKO_005 374 3UTR 100% 4.950 3.465 N PPAT n/a
9 TRCN0000075216 CCTTATGGAAATCGTCCCTTA pLKO.1 653 3UTR 100% 4.050 2.835 N PPAT n/a
10 TRCN0000075213 CCTGTCTAACTGTAGACAAAT pLKO.1 2062 3UTR 100% 1.320 0.792 N PPAT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_156493.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06755 pDONR223 100% 39.8% None (many diffs) n/a
2 ccsbBroad304_06755 pLX_304 37% 39.8% V5 (many diffs) n/a
3 TRCN0000470553 CCTATGAGTAACCACGGAACCCAA pLX_317 26.2% 39.8% V5 (many diffs) n/a
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