Construct: ORF ccsbBroad304_06769

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010151.1_s304c1, BRDN0000404808
Derived from:
ccsbBroadEn_06769
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRIM2 (5558)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06769
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5558 PRIM2 DNA primase subunit 2 NM_000947.5 99.9% 99.8% 870G>T
2 human 5558 PRIM2 DNA primase subunit 2 XM_017011004.2 88.7% 88.6% 693_884del;1062G>T
3 human 5558 PRIM2 DNA primase subunit 2 XM_017011005.2 72.8% 71.9% (many diffs)
4 human 5558 PRIM2 DNA primase subunit 2 XM_017011006.2 59.3% 59.1% (many diffs)
5 human 5558 PRIM2 DNA primase subunit 2 XM_011514731.3 56.8% 55% (many diffs)
6 human 5558 PRIM2 DNA primase subunit 2 XM_024446483.1 52.3% 50.3% 0_1ins658;35_36ins68;144G>T
7 human 101930420 LOC101930420 DNA primase large subunit-like XM_017007609.2 45.3% 35.7% (many diffs)
8 human 101930420 LOC101930420 DNA primase large subunit-like XM_017030301.2 45.3% 35.7% (many diffs)
9 human 5558 PRIM2 DNA primase subunit 2 NM_001282487.2 30.8% 30.2% (many diffs)
10 human 5558 PRIM2 DNA primase subunit 2 NM_001282488.1 30.8% 30.2% (many diffs)
11 human 107984021 LOC107984021 uncharacterized LOC107984021 XR_001740805.2 28.4% (many diffs)
12 human 107984021 LOC107984021 uncharacterized LOC107984021 XR_001756820.2 28.4% (many diffs)
13 mouse 19076 Prim2 DNA primase, p58 subunit NM_008922.2 83.7% 89.1% (many diffs)
14 mouse 19076 Prim2 DNA primase, p58 subunit XM_011238455.2 48.7% 48% (many diffs)
15 mouse 19076 Prim2 DNA primase, p58 subunit XM_006495797.2 37.8% 40.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1593
ORF length:
1527
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gttttctgga agaaagtgga ggaagctgag gttggcaggt gaccagagga
121atgcttccta ccctcattgc cttcagtttt acttgcagcc accttctgaa aacatatctt
181taatagaatt tgaaaacttg gctattgata gagttaaatt gttaaaatca gttgaaaatc
241ttggagtgag ctatgtgaaa ggaactgaac aataccagag taagttggag agtgagcttc
301ggaagctcaa gttttcctac agagaaaact tagaagatga atatgaacca cgaagaagag
361atcatatttc tcattttatt ttgcggcttg cttattgcca gtctgaagaa cttagacgct
421ggttcattca acaagaaatg gatctccttc gatttagatt tagtatttta cccaaggata
481aaattcagga tttcttaaag gatagccaat tgcagtttga ggctataagt gatgaagaga
541agactcttcg agaacaggag attgttgcct catcaccaag tttaagtgga cttaagttgg
601ggttcgagtc catttataag atcccttttg ctgatgctct ggatttgttt cgaggaagga
661aagtctattt ggaagatggc tttgcttacg taccacttaa ggacattgtg gcaatcatcc
721tgaatgaatt tagagccaaa ctgtccaagg ctttggcatt aacagccagg tccttgcctg
781ctgtgcagtc tgatgaaaga cttcagcctc tgctcaatca cctcagtcat tcctacactg
841gccaagatta cagtacccag ggaaatgttg ggaagatttc tttagatcag attgatttgc
901tttctaccaa atccttccca ccttgcatgc gtcatttaca taaagccttg cgggaaaatc
961accatcttcg tcatggaggc cgaatgcagt atggcctatt tctgaagggc attggtttaa
1021ctttggaaca ggcattgcag ttctggaagc aagaatttat caaaggaaag atggatccag
1081acaagtttga taaaggttac tcttacaaca tccgtcacag ctttggaaag gaaggcaaga
1141ggacagacta tacacctttc agttgcctga agattattct gtccaatcca ccaagccaag
1201gggattatca tgggtgccca ttccgtcaca gtgatccaga gctgctgaag caaaagttgc
1261agtcatacaa gatctctcct ggagggataa gccagatttt ggatttagta aaggggacac
1321attaccaggt agcctgtcaa aaatactttg agatgataca caatgtggat gattgtggct
1381tttctttgaa tcatcctaat cagttctttt gtgagagcca acgtattcta aatggtggta
1441aagacataaa gaaggaacct atccaaccag aaactcctca acccaaacca agtgtccaga
1501aaaccaagga tgcatcatct gctctggcct ctttaaattc ctctctggaa atggatatgg
1561aaggactaga agattacttt agtgaagatt cttacccaac tttcttgtac aaagtggttg
1621gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
1681accggtggcg cgttaagtcg acaatcaacc tctggatta

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