Construct: ORF ccsbBroad304_06770

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002484.1_s304c1, BRDN0000400672
Derived from:
ccsbBroadEn_06770
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKAA1 (5562)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06770
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5562 PRKAA1 protein kinase AMP-activate... NM_206907.3 99.9% 99.8% 28A>T
2 human 5562 PRKAA1 protein kinase AMP-activate... NM_006251.5 97.3% 97.2% 28A>T;362_363ins45
3 human 5562 PRKAA1 protein kinase AMP-activate... XM_017009624.1 94.2% 92% (many diffs)
4 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355028.2 91.6% 89.4% (many diffs)
5 human 5562 PRKAA1 protein kinase AMP-activate... XM_017009625.2 91.6% 89.4% (many diffs)
6 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355029.2 72.2% 72.2% 0_1ins477
7 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355035.2 52.4% 52.4% 0_1ins819
8 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355036.2 52.4% 52.4% 0_1ins819
9 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355037.2 52.4% 52.4% 0_1ins819
10 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355034.1 36.4% 33.9% (many diffs)
11 mouse 105787 Prkaa1 protein kinase, AMP-activat... NM_001013367.3 87.8% 95.9% (many diffs)
12 mouse 105787 Prkaa1 protein kinase, AMP-activat... XM_011245321.2 71.8% 78.5% (many diffs)
13 mouse 105787 Prkaa1 protein kinase, AMP-activat... XM_017316356.1 71.8% 78.5% (many diffs)
14 mouse 105787 Prkaa1 protein kinase, AMP-activat... XM_006519953.2 64.8% 71% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1788
ORF length:
1722
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg cagactcagt tcctggagaa agttggcgac agccgagaag cagaaacacg
121acgggcgggt gaagatcggc cactacattc tgggtgacac gctgggggtc ggcaccttcg
181gcaaagtgaa ggttggcaaa catgaattga ctgggcataa agtagctgtg aagatactca
241atcgacagaa gattcggagc cttgatgtgg taggaaaaat ccgcagagaa attcagaacc
301tcaagctttt caggcatcct catataatta aactgtacca ggtcatcagt acaccatctg
361atattttcat ggtgatggaa tatgtctcag gaggagagct atttgattat atctgtaaga
421atggaaggaa atctgatgta cctggagtag taaaaacagg ctccacgaag gagctggatg
481aaaaagaaag tcggcgtctg ttccaacaga tcctttctgg tgtggattat tgtcacaggc
541atatggtggt ccatagagat ttgaaacctg aaaatgtcct gcttgatgca cacatgaatg
601caaagatagc tgattttggt ctttcaaaca tgatgtcaga tggtgaattt ttaagaacaa
661gttgtggctc acccaactat gctgcaccag aagtaatttc aggaagattg tatgcaggcc
721cagaggtaga tatatggagc agtggggtta ttctctatgc tttattatgt ggaacccttc
781catttgatga tgaccatgtg ccaactcttt ttaagaagat atgtgatggg atcttctata
841cccctcaata tttaaatcct tctgtgatta gccttttgaa acatatgctg caggtggatc
901ccatgaagag ggccacaatc aaagatatca gggaacatga atggtttaaa caggaccttc
961caaaatatct ctttcctgag gatccatcat atagttcaac catgattgat gatgaagcct
1021taaaagaagt atgtgaaaag tttgagtgct cagaagagga agttctcagc tgtctttaca
1081acagaaatca ccaggatcct ttggcagttg cctaccatct cataatagat aacaggagaa
1141taatgaatga agccaaagat ttctatttgg cgacaagccc acctgattct tttcttgatg
1201atcatcacct gactcggccc catcctGAAA GAGTACCATT CTTGGTTGCT GAAACACCAA
1261GGGCACGCCA TACCCTTGAT GAATTAAATC CACAGAAATC CAAACACCAA GGTGTAAGGA
1321AAGCAAAATG GCATTTAGGA ATTAGAAGTC AAAGTCGACC AAATGATATT ATGGCAGAAG
1381TATGTAGAGC AATCAAACAA TTGGATTATG AATGGAAGGT TGTAAACCCA TATTATTTGC
1441GTGTACGAAG GAAGAATCCT GTGACAAGCA CTTACTCCAA AATGAGTCTA CAGTTATACC
1501AAGTGGATAG TAGAACTTAT CTACTGGATT TCCGTAGTAT TGATGATGAA ATTACAGAAG
1561CCAAATCAGG GACTGCTACT CCACAGAGAT CGGGATCAGT TAGCAACTAT CGATCTTGCC
1621AAAGGAGTGA TTCAGATGCT GAGGCTCAAG GAAAATCCTC AGAAGTTTCT CTTACCTCAT
1681CTGTGACCTC ACTTGACTCT TCTCCTGTTG ACCTAACTCC AAGACCTGGA AGTCACACAA
1741TAGAATTTTT TGAGATGTGT GCAAATCTAA TTAAAATTCT TGCACAATAC CCAACTTTCT
1801TGTACaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1861aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)