Construct: ORF ccsbBroad304_06772

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011457.1_s304c1, BRDN0000410047
Derived from:
ccsbBroadEn_06772
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKACG (5568)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06772
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5568 PRKACG protein kinase cAMP-activat... NM_002732.3 99.8% 99.7% 12C>T;1033G>C
2 human 5566 PRKACA protein kinase cAMP-activat... NM_002730.4 85.2% 82.9% (many diffs)
3 human 5566 PRKACA protein kinase cAMP-activat... NM_207518.3 82.9% 80.6% (many diffs)
4 human 5566 PRKACA protein kinase cAMP-activat... XM_017026948.1 72.5% 70% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1119
ORF length:
1053
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg caacgctccc gccaagaagg acaccgagca ggaggagagc gtgaacgagt
121tcctagccaa agccagagga gatttcctct acagatgggg aaaccccgct caaaacaccg
181ccagctcgga tcagttcgaa cggctcagga cgctgggcat gggctccttc gggcgggtga
241tgctggtgag gcaccaggag accggcggcc actacgccat gaagatcctc aacaagcaga
301aggtggtgaa gatgaagcag gtcgagcaca tactgaacga gaagcgcatc ctgcaggcga
361tcgactttcc gttcctcgtc aagctccagt tctcctttaa ggacaactcc tacctgtacc
421tggtgatgga gtacgtgccg ggtggggaga tgttctcccg cctacagcgc gtcggaaggt
481ttagcgagcc ccatgcctgt ttctatgccg cccaggtcgt cctggccgtc cagtacctac
541actcgctcga cctcatccac cgcgacctGA AGCCCGAGAA TCTCCTCATC GACCAGCAGG
601GCTACCTGCA GGTGACGGAC TTCGGTTTCG CCAAGCGCGT GAAGGGCCGC ACTTGGACCT
661TGTGCGGGAC CCCAGAGTAC CTGGCCCCCG AGATCATCCT GAGCAAAGGC TACAACAAGG
721CCGTGGACTG GTGGGCCCTA GGGGTGCTCA TCTATGAGAT GGCCGTGGGC TTCCCACCCT
781TCTACGCCGA CCAGCCCATC CAGATCTACG AGAAGATCGT CTCTGGGAGG GTGCGGTTTC
841CCTCCAAACT CAGCTCTGAC CTCAAGCATC TGCTGCGGAG CCTGCTGCAG GTGGACCTCA
901CCAAGCGCTT CGGAAACCTC AGGAACGGGG TTGGCGACAT CAAGAACCAC AAGTGGTTCG
961CCACAACCAG CTGGATCGCC ATCTATGAGA AGAAGGTGGA AGCTCCCTTC ATCCCGAAGT
1021ACACAGGCCC TGGGGATGCC AGTAACTTTG ACGACTACGA GGAGGAAGAG CTCCGGATCT
1081CCATCAATGA GAAGTGTCCC AAGGAGTTTT CTGAGTTTTA CCCAACTTTC TTGTACaaag
1141tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1201gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)