Construct: ORF ccsbBroad304_06812
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017960.1_s304c1, BRDN0000408227
- Derived from:
- ccsbBroadEn_06812
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTH1R (5745)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06812
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | NM_000316.3 | 99.9% | 100% | 1389T>C |
| 2 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | NM_001184744.1 | 99.9% | 100% | 1389T>C |
| 3 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006933.1 | 99.9% | 100% | 1389T>C |
| 4 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_011533967.3 | 97.7% | 97.8% | 175_213del;1428T>C |
| 5 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006932.2 | 97.7% | 97.8% | 175_213del;1428T>C |
| 6 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_011533968.2 | 95% | 92.5% | (many diffs) |
| 7 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_005265344.3 | 94.7% | 94.7% | 0_1ins93;1296T>C |
| 8 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006934.1 | 82.9% | 68.6% | (many diffs) |
| 9 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_001083935.1 | 86.5% | 90.2% | (many diffs) |
| 10 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_001083936.1 | 86.5% | 90.2% | (many diffs) |
| 11 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_011199.2 | 86.5% | 90.2% | (many diffs) |
| 12 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511988.3 | 84.2% | 85.7% | (many diffs) |
| 13 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_017313215.1 | 84.2% | 85.7% | (many diffs) |
| 14 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511987.2 | 82.8% | 84.2% | (many diffs) |
| 15 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_017313214.1 | 78.8% | 78.8% | (many diffs) |
| 16 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511985.2 | 69.7% | 69.9% | (many diffs) |
| 17 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_011242942.1 | 56% | 55.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1848
- ORF length:
- 1779
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggaccgcc cggatcgcac ccggcctggc gctcctgctc tgctgccccg 121 tgctcagctc cgcgtacgcg ctggtggatg cagatgacgt catgactaaa gaggaacaga 181 tcttcctgct gcaccgtgct caggcccagt gcgaaaaacg gctcaaggag gtcctgcaga 241 ggccagccag cataatggaa tcagacaagg gatggacatc tgcgtccaca tcagggaagc 301 ccaggaaaga taaggcatct gggaagctct accctgagtc tgaggaggac aaggaggcac 361 ccactggcag caggtaccga gggcgcccct gtctgccgga atgggaccac atcctgtgct 421 ggccgctggg ggcaccaggt gaggtggtgg ctgtgccctg tccggactac atttatgact 481 tcaatcacaa aggccatgcc taccgacgct gtgaccgcaa tggcagctgg gagctggtgc 541 ctgggcacaa caggacgtgg gccaactaca gcgagtgtgt caaatttctc accaatgaga 601 ctcgtgaacg ggaggtgttt gaccgcctgg gcatgattta caccgtgggc tactccgtgt 661 ccctggcgtc cctcaccgta gctgtgctca tcctggccta ctttaggcgg ctgcactgca 721 cgcgcaacta catccacatg cacctgttcc tgtccttcat gctgcgcgcc gtgagcatct 781 tcgtcaagga cgctgtgctc tactctggcg ccacgcttga tgaggctgag cgcctcaccg 841 aggaggagct gcgcgccatc gcccaggcgc ccccgccgcc tgccaccgcc gctgccggct 901 acgcgggctg cagggtggct gtgaccttct tcctttactt cctggccacc aactactact 961 ggattctggt ggaggggctg tacctgcaca gcctcatctt catggccttc ttctcagaga 1021 agaagtacct gtggggcttc acagtcttcg gctggggtct gcccgctgtc ttcgtggctg 1081 tgtgggtcag tgtcagagct accctggcca acaccgggtg ctgggacttg agctccggga 1141 acaaaaagtg gatcatccag gtgcccatcc tggcctccat tgtgctcaac ttcatcctct 1201 tcatcaatat cgtccgggtg ctcgccacca agctgcggga gaccaacgcc ggccggtgtg 1261 acacacggca gcagtaccgg aagctgctca aatccacgct ggtgctcatg cccctctttg 1321 gcgtccacta cattgtcttc atggccacac catacaccga ggtctcaggg acgctctggc 1381 aagtccagat gcactatgag atgctcttca actccttcca gggatttttt gtcgcaatca 1441 tatactgttt ctgcaacggc gaggtacaag ctgagatcaa gaaatcttgg agccgctgga 1501 cactggcact ggacttcaag cgaaaggcac gcagcgggag cagcagctat agctacggcc 1561 ccatggtgtc ccacacaagt gtgaccaatg tcggcccccg tgtgggactc ggcctgcccc 1621 tcagcccccg cctactgccc actgccacca ccaacggcca ccctcagctg cctggccatg 1681 ccaagccagg gaccccagcc ctggagaccc tcgagaccac accacctgcc atggctgctc 1741 ccaaggacga tgggttcctc aacggctcct gctcaggcct ggacgaggag gcctctgggc 1801 ctgagcggcc acctgccctg ctacaggaag agtgggagac agtcatgttg ccaactttct 1861 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1921 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta