Transcript: Mouse XM_006511985.2

PREDICTED: Mus musculus parathyroid hormone 1 receptor (Pth1r), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pth1r (19228)
Length:
2370
CDS:
31..2232

Additional Resources:

NCBI RefSeq record:
XM_006511985.2
NBCI Gene record:
Pth1r (19228)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006511985.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000027965 GCATTGGACTTCAAGCGTAAA pLKO.1 1894 CDS 100% 10.800 15.120 N Pth1r n/a
2 TRCN0000425351 GCACATGTTCCTGTCGTTTAT pLKO_005 1128 CDS 100% 13.200 10.560 N Pth1r n/a
3 TRCN0000416423 TGATCTACACCGTGGGATATT pLKO_005 1022 CDS 100% 13.200 9.240 N Pth1r n/a
4 TRCN0000027983 CTCCAGCCATTGAGAACGAAA pLKO.1 2078 CDS 100% 4.950 3.465 N Pth1r n/a
5 TRCN0000027985 GCTACCAACTACTACTGGATT pLKO.1 1333 CDS 100% 4.950 3.465 N Pth1r n/a
6 TRCN0000028004 GCAATGTGACAAGCTGCTCAA pLKO.1 594 CDS 100% 4.050 2.835 N Pth1r n/a
7 TRCN0000027953 GAGTCTAAAGAGAACAAGGAT pLKO.1 724 CDS 100% 3.000 2.100 N Pth1r n/a
8 TRCN0000003302 CAACTACTACTGGATTCTGGT pLKO.1 1338 CDS 100% 2.640 1.848 N PTH1R n/a
9 TRCN0000378220 TTGACCGCCTGGGCATGATTT pLKO_005 1007 CDS 100% 13.200 9.240 N PTH1R n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006511985.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06812 pDONR223 100% 69.7% 69.9% None (many diffs) n/a
2 ccsbBroad304_06812 pLX_304 0% 69.7% 69.9% V5 (many diffs) n/a
3 TRCN0000480580 AGTTTTACACCGCCACACTGTTTC pLX_317 20.4% 69.7% 69.9% V5 (many diffs) n/a
4 TRCN0000489527 AGTACACTGCCAGCCCCGCGCCTG pLX_317 19.5% 69.7% 69.8% V5 (many diffs) n/a
5 TRCN0000488371 GCAGTTCCGCTTAGCTTTACGGGG pLX_317 19.6% 69.7% 69.9% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_15552 pDONR223 0% 69.7% 69.9% None (many diffs) n/a
7 ccsbBroad304_15552 pLX_304 0% 69.7% 69.9% V5 (many diffs) n/a
8 ccsbBroadEn_11070 pDONR223 100% 39.4% 38% None (many diffs) n/a
9 ccsbBroad304_11070 pLX_304 0% 39.4% 38% V5 (many diffs) n/a
10 TRCN0000474180 CTCCAAGACGGCAAAGGCGTAGCA pLX_317 48.4% 39.4% 38% V5 (many diffs) n/a
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