Construct: ORF ccsbBroad304_06867
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011854.1_s304c1, BRDN0000399625
- Derived from:
- ccsbBroadEn_06867
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF2 (6045)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06867
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6045 | RNF2 | ring finger protein 2 | NM_007212.4 | 99.9% | 99.7% | 4T>A |
| 2 | human | 6045 | RNF2 | ring finger protein 2 | XM_011509851.3 | 99.9% | 99.7% | 4T>A |
| 3 | human | 6045 | RNF2 | ring finger protein 2 | XM_011509852.2 | 99.9% | 99.7% | 4T>A |
| 4 | human | 6045 | RNF2 | ring finger protein 2 | XM_005245413.3 | 85.4% | 85.4% | 0_1ins147 |
| 5 | mouse | 19821 | Rnf2 | ring finger protein 2 | NM_011277.2 | 92% | 99.4% | (many diffs) |
| 6 | mouse | 19821 | Rnf2 | ring finger protein 2 | XM_006529269.3 | 89.6% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1074
- ORF length:
- 1008
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac tcaggctgtg cagacaaacg gaactcaacc attaagcaaa acatgggaac 121 tcagtttata tgagttacaa cgaacacctc aggaggcaat aacagatggc ttagaaattg 181 tggtttcacc tcgaagtcta cacagtgaat taatgtgccc aatttgtttg gatatgttga 241 agaacaccat gactacaaag gagtgtttac atcgtttttg tgcagactgc atcatcacag 301 cccttagaag tggcaacaaa gaatgtccta cctgtcggaa aaaactagtt tccaaaagat 361 cactaaggcc agacccaaac tttgatgcac tcatcagcaa aatttatcca agtcgtgatg 421 agtatgaagc tcatcaagag agagtattag ccaggatcaa caagcaCAAT AATCAGCAAG 481 CACTCAGTCA CAGCATTGAG GAAGGACTGA AGATACAGGC CATGAACAGA CTGCAGCGAG 541 GCAAGAAACA ACAGATTGAA AATGGTAGTG GAGCAGAAGA TAATGGTGAC AGTTCACACT 601 GCAGTAATGC ATCCACACAT AGCAATCAGG AAGCAGGCCC TAGTAACAAA CGGACCAAAA 661 CATCTGATGA TTCTGGGCTA GAGCTTGATA ATAACAATGC AGCAATGGCA ATTGATCCAG 721 TAATGGATGG TGCTAGTGAA ATTGAATTAG TATTCAGGCC TCATCCCACA CTTATGGAAA 781 AAGATGACAG TGCACAGACG AGATACATAA AGACTTCTGG TAACGCCACT GTTGATCACT 841 TATCCAAGTA TCTGGCTGTG AGGTTAGCTT TAGAAGAACT TCGAAGCAAA GGTGAATCAA 901 ACCAGATGAA CCTTGATACA GCCAGTGAGA AGCAGTATAC CATTTATATA GCAACAGCCA 961 GTGGCCAGTT CACTGTATTA AATGGCTCTT TTTCTTTGGA ATTGGTCAGT GAGAAATACT 1021 GGAAAGTGAA CAAACCCATG GAACTTTATT ACGCACCTAC AAAGGAGCAC AAATGCCCAA 1081 CTTTCTTGTA Caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1141 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta