Construct: ORF ccsbBroad304_06908
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015923.1_s304c1, BRDN0000403649
- Derived from:
- ccsbBroadEn_06908
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- S100B (6285)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06908
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6285 | S100B | S100 calcium binding protein B | NM_006272.3 | 99.6% | 100% | 99G>C |
| 2 | human | 6285 | S100B | S100 calcium binding protein B | XM_017028424.2 | 99.6% | 100% | 99G>C |
| 3 | mouse | 20203 | S100b | S100 protein, beta polypept... | NM_009115.3 | 85.1% | 98.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 342
- ORF length:
- 276
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgagctggag aaggccatgg tggccctcat cgacgttttc caccaatatt 121 ctggaaggga gggagacaag cacaagctga agaaatccga actcaaggag ctcatcaaca 181 atgagctttc ccatttctta gaggaaatca aagagcagga ggttgtggac aaagtcatgg 241 aaacactgga caatgatgga gacggcgaat gtgacttcca ggaattcatg gcctttgttg 301 ccatggttac tactgcctgc cacgagttct ttgaacatga gtgcccaact ttcttgtaca 361 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 421 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta