Construct: ORF ccsbBroad304_06944
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006241.1_s304c1, BRDN0000409868
- Derived from:
- ccsbBroadEn_06944
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3BGR (6450)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06944
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6450 | SH3BGR | SH3 domain binding glutamat... | NM_007341.2 | 99.8% | 99.5% | 69C>G |
| 2 | human | 6450 | SH3BGR | SH3 domain binding glutamat... | NM_001317740.1 | 86.8% | 86.6% | 69C>G;501_502ins93 |
| 3 | human | 106865373 | WRB-SH3BGR | WRB-SH3BGR readthrough | NM_001317744.2 | 72.8% | 58.9% | (many diffs) |
| 4 | human | 6450 | SH3BGR | SH3 domain binding glutamat... | NM_001001713.1 | 53.5% | 53.5% | 0_1ins333 |
| 5 | human | 6450 | SH3BGR | SH3 domain binding glutamat... | NM_001317742.1 | 53.5% | 53.5% | 0_1ins333 |
| 6 | human | 6450 | SH3BGR | SH3 domain binding glutamat... | NM_001317741.1 | 40.5% | 40.5% | 0_1ins333;168_169ins93 |
| 7 | human | 106865373 | WRB-SH3BGR | WRB-SH3BGR readthrough | NR_146618.2 | 28.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc tctgctgctc cttggagaga cagagcctct taagttggag cgggactgcc 121 ggagcccagt ggagccctgg gctgctgcca gccccgacct ggcacttgct tgcctgtgtc 181 actgtcagga tttgtctagc ggcgcattcc ctaacagggg tgtgttgggg ggagtcctct 241 ttccaactgt cgaaatggtt atcaaagtgt ttgttgctac atcttctggg tccatagcga 301 ttaggaagaa acagcaagaa gtagtgggtt ttttggaagc gaataaaatc gactttaagg 361 aattagatat tgctggagat gaagacaaca ggaggtggat gagagagaat gttcctggag 421 agaaaaaacc tcaaaatggg attcctttgc ctccccagat cttcaatgag gagcagtact 481 gtggggattt tgactctttc ttctctgcaa aagaagagaa tattatttat tccttccttg 541 gtttggctcc tcctccagac tcaaagggat cagagaaggc tgaagaaggt ggagaaactg 601 aggcacaaaa agagggcagt gaagatgtgg gcaacctccc tgaagcccag gagaagaatg 661 aagaagaagg agagacagcc acagaagaga cggaagaaat agccatggag ggtgcggaag 721 gggaagccga ggaggaggaa gaaactgcag aaggagaaga gcctggagaa gacgaagatt 781 cctacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 841 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 901 tctggatta