Construct: ORF ccsbBroad304_06951

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002705.1_s304c1, BRDN0000399833
Derived from:
ccsbBroadEn_06951
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SIX1 (6495)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06951
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6495 SIX1 SIX homeobox 1 NM_005982.4 99.8% 99.6% 295C>T
2 human 6495 SIX1 SIX homeobox 1 XM_017021602.2 60.7% 59.1% 295C>T;499_500ins59;519_520ins274
3 mouse 20471 Six1 sine oculis-related homeobox 1 NM_009189.3 93.1% 98.9% (many diffs)
4 mouse 20472 Six2 sine oculis-related homeobox 2 XM_011246335.2 61.4% 65.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
918
ORF length:
852
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc gatgctgccg tcgtttggct ttacgcagga gcaagtggcg tgcgtgtgcg
121aggttctgca gcaaggcgga aacctggagc gcctgggcag gttcctgtgg tcactgcccg
181cctgcgacca cctgcacaag aacgagagcg tactcaaggc caaggcggtg gtcgccttcc
241accgcggcaa cttccgtgag ctctacaaga tcctggagag ccaccagttc tcgcctcaca
301accaccccaa actgcagcaa ctgtggctga aggcgcatta cgtggaggcc gagaagctgt
361gcggccgacc cctgggcgcc gtgggcaaat atcgggtgcg ccgaaaattt ccactgccgc
421gcaccatctg ggacggcgag gagaccagct actgcttcaa ggagaagtcg aggggtgtcc
481tgcgggagtg gtacgcgcac aatccctacc catcgccgcg tgagaagcgg gagctggccg
541aggccaccgg cctcaccacc acccaggtca gcaactggtt taagaaccgg aggcaaagag
601accgggccgc ggaggccaag gaaagggaga acaccgaaaa caataactcc tcctccaaca
661agcagaacca actctctcct ctggaagggg gcaagccgct catgtccagc tcagaagagg
721aattctcacc tccccaaagt ccagaccaga actcggtcct tctgctgcag ggcaatatgg
781gccacgccag gagctcaaac tattctctcc cgggcttaac agcctcgcag cccagtcacg
841gcctgcagac ccaccagcat cagctccaag actctctgct cggccccctc acctccagtc
901tggtggactt ggggtcctac ccaactttct tgtacaaagt ggttggtaag cctatcccta
961accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
1021agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)