Construct: ORF ccsbBroad304_06960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005519.1_s304c1, BRDN0000461973
- Derived from:
- ccsbBroadEn_06960
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC5A5 (6528)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06960
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6528 | SLC5A5 | solute carrier family 5 mem... | NM_000453.3 | 99.9% | 100% | 657G>A |
| 2 | human | 6528 | SLC5A5 | solute carrier family 5 mem... | XM_011528192.2 | 98.2% | 98.3% | 657G>A;966_998del |
| 3 | human | 6528 | SLC5A5 | solute carrier family 5 mem... | XM_017027158.1 | 86.1% | 86.1% | 0_1ins267;390G>A |
| 4 | human | 6528 | SLC5A5 | solute carrier family 5 mem... | XM_011528193.3 | 84.6% | 84.7% | 0_1ins267;390G>A;699_731del |
| 5 | human | 6528 | SLC5A5 | solute carrier family 5 mem... | XM_011528194.3 | 79.6% | 79.6% | 0_1ins366;291G>A;600_632del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1998
- ORF length:
- 1929
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggccgtg gagaccgggg aacggcccac cttcggagcc tgggactacg 121 gggtctttgc cctcatgctc ctggtgtcca ctggcatcgg gctgtgggtc gggctggctc 181 ggggcgggca gcgcagcgct gaggacttct tcaccggggg ccggcgcctg gcggccctgc 241 ccgtgggcct gtcgctgtct gccagcttca tgtcggccgt gcaggtgctg ggcgtgccgt 301 cggaggccta tcgctatggc ctcaagttcc tctggatgtg cctgggccag cttctgaact 361 cggtcctcac cgccctgctc ttcatgcccg tcttctaccg cctgggcctc accagcacct 421 acgagtacct ggagatgcgc ttcagccgcg cagtgcggct ctgcgggact ttgcagtaca 481 ttgtagccac gatgctgtac accggcatcg taatctacgc accggccctc atcctgaacc 541 aagtgaccgg gctggacatc tgggcgtcgc tcctgtccac cggaattatc tgcaccttct 601 acacggctgt gggcggcatg aaggctgtgg tctggactga tgtgttccag gtcgtggtga 661 tgctaagtgg cttctgggtt gtcctggcac gcggtgtcat gcttgtgggc gggccccgcc 721 aggtactcac gctggcccag aaccactccc ggatcaacct catggacttt aaccctgacc 781 cgaggagccg ctatacattc tggacttttg tggtgggtgg cacgttggtg tggctctcca 841 tgtatggcgt gaaccaggcg caggtgcagc gctacgtggc ttgccgcaca gagaagcagg 901 ccaagctggc cctgctcatc aaccaggtcg gcctgttcct gatcgtgtcc agcgctgcct 961 gctgtggcat cgtcatgttt gtgttctaca ctgactgcga ccctctcctc ctggggcgca 1021 tctctgcccc agaccagtac atgcctctgc tggtgctgga catcttcgaa gatctgcctg 1081 gagtccccgg gcttttcctg gcctgtgctt acagtggcac cctcagcaca gcatccacca 1141 gcatcaatgc tatggctgca gtcactgtag aagacctcat caaacctcgg ctgcggagcc 1201 tggcacccag gaaactcgtg attatctcca aggggctctc actcatctac ggatcggcct 1261 gtctcaccgt ggcagccctg tcctcactgc tcggaggagg tgtccttcag ggctccttca 1321 ccgtcatggg agtcatcagc ggccccctgc tgggagcctt catcttggga atgttcctgc 1381 cggcctgcaa cacaccgggc gtcctcgcgg gactaggcgc gggcttggcg ctgtcgctgt 1441 gggtggcctt gggcgccacg ctgtacccac ccagcgagca gaccatgagg gtcctgccat 1501 cgtcggctgc ccgctgcgtg gctctctcag tcaacgcctc tggcctcctg gacccggctc 1561 tcctccctgc taacgactcc agcagggccc ccagctcagg aatggacgcc agccgacccg 1621 ccttagctga cagcttctat gccatctcct atctctatta cggtgccctg ggcacgctga 1681 ccactgtgct gtgcggagcc ctcatcagct gcctgacagg ccccaccaag cgcagcaccc 1741 tggccccggg attgttgtgg tgggacctcg cacggcagac agcatcagtg gcccccaagg 1801 aagaagtggc catcctggat gacaacttgg tcaagggtcc tgaagaactc cccactggaa 1861 acaagaagcc ccctggcttc ctgcccacca atgaggatcg tctgtttttc ttggggcaga 1921 aggagctgga gggggctggc tcttggaccc cctgtgttgg acatgatggt ggtcgagacc 1981 agcaggagac aaacctcttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 2041 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2101 agtcgacaat caacctctgg atta