Construct: ORF ccsbBroad304_06993

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005235.1_s304c1, BRDN0000399746
Derived from:
ccsbBroadEn_06993
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SRD5A1 (6715)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06993
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6715 SRD5A1 steroid 5 alpha-reductase 1 NM_001047.4 99.8% 100% 90C>G
2 human 6715 SRD5A1 steroid 5 alpha-reductase 1 NM_001324322.2 75.8% 48.4% 1_26del;116C>G;318_319ins167
3 human 6715 SRD5A1 steroid 5 alpha-reductase 1 NM_001324323.2 67.6% 63.7% (many diffs)
4 human 6719 SRD5A1P1 steroid 5 alpha-reductase 1... NR_028597.1 34.3% (many diffs)
5 human 6715 SRD5A1 steroid 5 alpha-reductase 1 NR_136739.2 10.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
843
ORF length:
777
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc aacggcgacg ggggtggcgg aggagcgcct gctggccgcg ctcgcctacc
121tgcagtgcgc cgtgggctgc gcggtcttcg cgcggaatcg tcagacgaac tcagtgtacg
181gccgccacgc gctgcccagc cacaggctcc gagtgccggc gcgggccgcc tgggtggtgc
241aggagctgcc ctcgctggcc ctgccgctct accagtacgc cagcgagtcc gccccgcgtc
301tccgcagcgc gcccaactgc atcctcctgg ccatgttcct cgtccactac gggcatcggt
361gcttaattta cccatttctg atgcgaggag gaaagcctat gccactgttg gcgtgtacaa
421tggcgattat gttctgtacc tgtaacggct atttgcaaag cagatacttg agccattgtg
481cagtgtatgc tgatgactgg gtaacagatc cccgttttct aataggtttt ggcttgtggt
541taacgggcat gttgataaac atccattcag atcatatcct aaggaatctc agaaaaccag
601gagatactgg atacaaaata ccaaggggag gcttatttga atacgtaact gcagccaact
661attttggaga aatcatggag tggtgtggct atgccctggc cagctggtct gtccaaggcg
721cggcttttgc tttcttcacg ttttgttttt tatctggtag agcaaaagag catcatgagt
781ggtacctccg gaaatttgaa gagtatccaa agttcagaaa aattataatt ccatttttgt
841tttacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
901tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
961tctggatta

Download FASTA (ORF) (Full)