Construct: ORF ccsbBroad304_06995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003941.1_s304c1, BRDN0000404857
- Derived from:
- ccsbBroadEn_06995
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SREBF1 (6720)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06995
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6720 | SREBF1 | sterol regulatory element b... | NM_004176.4 | 94.3% | 90.2% | (many diffs) |
| 2 | human | 6720 | SREBF1 | sterol regulatory element b... | NM_001321096.3 | 92.1% | 87.9% | (many diffs) |
| 3 | human | 6720 | SREBF1 | sterol regulatory element b... | XM_024450893.1 | 92% | 88.1% | (many diffs) |
| 4 | human | 6720 | SREBF1 | sterol regulatory element b... | NM_001005291.3 | 91.9% | 88% | (many diffs) |
| 5 | human | 6720 | SREBF1 | sterol regulatory element b... | XM_024450894.1 | 91.8% | 87.9% | (many diffs) |
| 6 | human | 6720 | SREBF1 | sterol regulatory element b... | XM_024450895.1 | 90% | 89.7% | (many diffs) |
| 7 | human | 6720 | SREBF1 | sterol regulatory element b... | XR_002958058.1 | 82.4% | (many diffs) | |
| 8 | human | 6720 | SREBF1 | sterol regulatory element b... | XM_017024970.2 | 55.5% | 52.3% | (many diffs) |
| 9 | human | 6720 | SREBF1 | sterol regulatory element b... | XM_017024971.1 | 53.6% | 50.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3561
- ORF length:
- 3495
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgagccaccc ttcagcgagg cggctttgga gcaggcgctg ggcgagccgt 121 gcgatctgga cgcggcgctg ctgaccgaca tcgaagacat gcttcagctt atcaacaacc 181 aagacagtga cttccctggc ctatttgacc caccctatgc tgggagtggg gcagggggca 241 cagaccctgc cagccccgat accagctccc caggcagctt gtctccacct cctgccacat 301 tgagctcctc tcttgaagcc ttcctgagcg ggccgcaggc agcgccctca cccctgtccc 361 ctccccagcc tgcacccact ccattgaaga tgtacccgtc catgcccgct ttctcccctg 421 ggcctggtat caaggaagag tcagtgccac tgagcatcct gcagaccccc accccacagc 481 ccctgccagg ggccctcctg ccacagagct tcccagcccc agccccaccg cagttcagct 541 ccacccctgt gttaggctac cccagccctc cgggaggctt ctctacagga agccctcccg 601 ggaacaccca gcagccgctg cctggcctgc cactggcttc cccgccaggg gtcccgcccg 661 tctccttgca cacccaggtc cagagtgtgg tcccccagca gctactgaca gtcacagctg 721 cccccacggc agcccctgta acgaccactg tgacctcgca gatccagcag gtcccggtcc 781 tgctgcagcc ccacttcatc aaggcagact cgctgcttct gacagccatg aagacagacg 841 gagccactgt gaaggcggca ggtctcagtc ccctggtctc tggcaccact gtgcagacag 901 ggcctttgcc gaccctggtg agtggcggaa ccatcttggc aacagtccca ctggtcgtag 961 atgcggagaa gctgcctatc aaccggctcg cagctggcag caaggccccg gcctctgccc 1021 agagccgtgg agagaagcgc acagcccaca acgccattga gaagcgctac cgctcctcca 1081 tcaatgacaa aatcattgag ctcaaggatc tggtggtggg cactgaggca aagctgaata 1141 aatctgctgt cttgcgcaag gccatcgact acattcgctt tctgcaacac agcaaccaga 1201 aactcaagca ggagaaccta agtctgcgca ctgctgtcca caaaagcaaa tctctgaagg 1261 atctggtgtc ggcctgtggc agtggaggga acacagacgt gctcatggag ggcgtgaaga 1321 ctgaggtgga ggacacactg accccacccc cctcggatgc tggctcacct ttccagagca 1381 gccccttgtc ccttggcagc aggggcagtg gcagcggtgg cagtggcagt gactcggagc 1441 ctgacagccc agtctttgag gacagcaagg caaagccaga gcagcggccg tctctgcaca 1501 gccggggcat gctggaccgc tcccgcctgg ccctgtgcac gctcgtcttc ctctgcctgt 1561 cctgcaaccc cttggcctcc ttgctggggg cccgggggct tcccagcccc tcagatacca 1621 ccagcgtcta ccatagccct gggcgcaacg tgctgggcac cgagagcaga gatggccctg 1681 gctgggccca gtggctgctg cccccagtgg tctggctgct caatgggctg ttggtgctcg 1741 tctccttggt gcttctcttt gtctacggtg agccagtcac acggccccac tcaggccccg 1801 ccgtgtactt ctggaggcat cgcaagcagg ctgacctgga cctggcccgg ggagactttg 1861 cccaggctgc ccagcagctg tggctggccc tgcgggcact gggccggccc ctgcccacct 1921 cccacctgga cctggcttgt agcctcctct ggaacctcat tcgtcacctg ctgcagcgtc 1981 tctgggtggg ccgctggctg gcaggccggg cagggggcct gcagcaggac tgtgctctgc 2041 gagtggatgc tagcgccagc gcccgagacg cagccctggt ctaccataag ctgcaccagc 2101 tgcacaccat ggggaagcac acaggcgggc acctcactgc caccaacctg gcgctgagtg 2161 ccctgaacct ggcagagtgt gcaggggatg ccgtgtctgt ggcgacgctg gccgagatct 2221 atgtggcggc tgcattgaga gtgaagacca gtctcccacg ggccttgcat tttctgacac 2281 gcttcttcct gagcagtgcc cgccaggcct gcctggcaca gagtggctca gtgcctcctg 2341 ccatgcagtg gctctgccac cccgtgggcc accgtttctt cgtggatggg gactggtccg 2401 tgctcagtac cccatgggag agcctgtaca gcttggccgg gaacccagtg gaccccctgg 2461 cccaggtgac tcagctattc cgggaacatc tcttagagcg agcactgaac tgtgtgaccc 2521 agcccaaccc cagccctggg tcagctgatg gggacaagga attctcggat gccctcgggt 2581 acctgcagct gctgaacagc tgttctgatg ctgcgggggc tcctgcctac agcttctcca 2641 tcagttccag catggccacc accaccggcg tagacccggt ggccaagtgg tgggcctctc 2701 tgacagctgt ggtgatccac tggctgcggc gggatgagga ggcggctgag cggctgtgcc 2761 cgctggtgga gcacctgccc cgggtgctgc aggagtctga gagacccctg cccagggcag 2821 ctctgcactc cttcaaggct gcccgggccc tgctgggctg tgccaaggca gagtctggtc 2881 cagccagcct gaccatctgt gagaaggcca gtgggtacct gcaggacagc ctggctacca 2941 caccagccag cagctccatt gacaaggccg tgcagctgtt cctgtgtgac ctgcttcttg 3001 tggtgcgcac cagcctgtgg cggcagcagc agcccccggc cccggcccca gcagcccagg 3061 gcaccagcag caggccccag gcttccgccc ttgagctgcg tggcttccaa cgggacctga 3121 gcagcctgag gcggctggca cagagcttcc ggcccgccat gcggagggtg ttcctacatg 3181 aggccacggc ccggctgatg gcgggggcca gccccacacg gacacaccag ctcctcgacc 3241 gcagtctgag gcggcgggca ggccccggtg gcaaaggagg cgcggtggcg gagctggagc 3301 TTCCCCGTAC GGGACTTCAA ATTCTTAGCA GGGGGAACGA AGAAAGCCGT AAATCTAAGA 3361 GAGGGCGGAG CCGTTTCCGC TCGGTGACTA ATACTTGCAC CAGAGCCAGA AACACTACGT 3421 CACGGAGCCC TGTTGAGAGT AGATCAGACT GTAGATTGGA AGGGGCCCAT GAAAACAAAG 3481 AAATATCGTC CACATTCTTC AAACGGGAGA AAGATTTCCC TCCCCACCCT GTCCCAGTAC 3541 AAAAAATATT CGTTCGAGGT AATTTGTCCC CTATTAACGG TCTATAGTAT ATCTAATTAC 3601 CAACTTTTTT GGAAAAAGGG GGGTGGTTAA CCCTCCCCCC ACCCCTCCCC TCGCGTGATT 3661 TGTATTAATA AGCTAGCGCT ATCCGCTGCC GCGTTAAGTC GACAATCAAC CTCTGGATTA 3721 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 3781 ATACGCTGCT TTAATGCCTT GTATCCATGT TTTTTCTGCT T