Construct: ORF ccsbBroad304_07024
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014286.1_s304c1, BRDN0000410241
- Derived from:
- ccsbBroadEn_07024
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYK (6850)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07024
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001135052.4 | 99.9% | 99.8% | 17T>A |
| 2 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001174168.3 | 99.9% | 99.8% | 17T>A |
| 3 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001174167.3 | 96.3% | 96.2% | 17T>A;846_914del |
| 4 | human | 6850 | SYK | spleen associated tyrosine ... | NM_003177.7 | 96.3% | 96.2% | 17T>A;846_914del |
| 5 | human | 6850 | SYK | spleen associated tyrosine ... | XM_005252147.4 | 96.3% | 96.2% | 17T>A;846_914del |
| 6 | human | 6850 | SYK | spleen associated tyrosine ... | XM_011518946.3 | 96.3% | 96.2% | 17T>A;846_914del |
| 7 | human | 6850 | SYK | spleen associated tyrosine ... | XR_001746370.2 | 36.2% | (many diffs) | |
| 8 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_006516897.4 | 84.3% | 92.1% | (many diffs) |
| 9 | mouse | 20963 | Syk | spleen tyrosine kinase | NM_001198977.1 | 82% | 88.8% | (many diffs) |
| 10 | mouse | 20963 | Syk | spleen tyrosine kinase | NM_011518.2 | 82% | 88.8% | (many diffs) |
| 11 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_006516895.4 | 82% | 88.8% | (many diffs) |
| 12 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_017315471.2 | 48.6% | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1902
- ORF length:
- 1836
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcagcggc aaggctgaca gcgccaacca cctgcccttc tttttcggca 121 acatcacccg ggaggaggca gaagattacc tggtccaggg gggcatgagt gatgggcttt 181 atttgctgcg ccagagccgc aactacctgg gtggcttcgc cctgtccgtg gcccacggga 241 ggaaggcaca ccactacacc atcgagcggg agctgaatgg cacctacgcc atcgccggtg 301 gcaggaccca tgccagcccc gccgacctct gccactacca ctcccaggag tctgatggcc 361 tggtctgcct cctcaagaag cccttcaacc ggccccaagg ggtgcagccc aagactgggc 421 cctttgagga tttgaaggaa aacctcatca gggaatatgt gaagcagaca tggaacctgc 481 agggtcaggc tctggagcag gccatcatca gtcagaagcc tcagctggag aagctgatcg 541 ctaccacagc ccatgaaaaa atgccttggt tccatggaaa aatctctcgg gaagaatctg 601 agcaaattgt cctgatagga tcaaagacaa atggaaagtt cctgatccga gccagagaca 661 acaacggctc ctacgccctg tgcctgctgc acgaagggaa ggtgctgcac tatcgcatcg 721 acaaagacaa gacagggaag ctctccatcc ccgagggaaa gaagttcgac acgctctggc 781 agctagtcga gcattattct tataaagcag atggtttgtt aagagttctt actgtcccat 841 gtcaaaaaat cggcacacag ggaaatgtta attttggagg ccgtccacaa cttccaggtt 901 cccatcctgc gtcctcccct gcccaaggga accggcaaga gagtactgtg tcattcaatc 961 cgtatgagcc agaacttgca ccctgggctg cagacaaagg cccccagaga gaagccctac 1021 ccatggacac agaggtgtac gagagcccct acgcggaccc cgaggagatc aggcccaagg 1081 aggtttacct ggaccgaaag ctgctgacgc tggaagacaa agaactgggc tctggtaatt 1141 ttggaactgt gaaaaagggc tactaccaaa tgaaaaaagt tgtgaaaacc gtggctgtga 1201 aaatactgaa aaacgaggcc aatgaccccg ctcttaaaga tgagttatta gcagaagcaa 1261 atgtcatgca gcagctggac aacccgtaca tcgtgcggat gatcgggata tgcgaggccg 1321 agtcctggat gctggttatg gagatggcag aacttggtcc cctcaataag tatttgcagc 1381 agaacagaca tgtcaaggat aagaacatca tagaactggt tcatcaggtt tccatgggca 1441 tgaagtactt GGAGGAGAGC AATTTTGTGC ACAGAGATCT GGCTGCAAGA AATGTGTTGC 1501 TAGTTACCCA ACATTACGCC AAGATCAGTG ATTTCGGACT TTCCAAAGCA CTGCGTGCTG 1561 ATGAAAACTA CTACAAGGCC CAGACCCATG GAAAGTGGCC TGTCAAGTGG TACGCTCCGG 1621 AATGCATCAA CTACTACAAG TTCTCCAGCA AAAGCGATGT CTGGAGCTTT GGAGTGTTGA 1681 TGTGGGAAGC ATTCTCCTAT GGGCAGAAGC CATATCGAGG GATGAAAGGA AGTGAAGTCA 1741 CCGCTATGTT AGAGAAAGGA GAGCGGATGG GGTGCCCTGC AGGGTGTCCA AGAGAGATGT 1801 ACGATCTCAT GAATCTGTGC TGGACATACG ATGTGGAAAA CAGGCCCGGA TTCGCAGCAG 1861 TGGAACTGCG GCTGCGCAAT TACTACTATG ACGTGGTGAA CTACCCAACT TTCTTGTACa 1921 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1981 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta