Construct: ORF ccsbBroad304_07060

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011127.1_s304c1, BRDN0000409483
Derived from:
ccsbBroadEn_07060
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TGIF1 (7050)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07060
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_173207.4 69.6% 67% (many diffs)
2 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_001278682.2 68.1% 66% (many diffs)
3 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_001278684.2 67.3% 66.5% (many diffs)
4 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_003244.4 67.3% 66.5% (many diffs)
5 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_173208.3 67.3% 66.5% (many diffs)
6 human 7050 TGIF1 TGFB induced factor homeobox 1 XM_017025958.1 67.3% 66.5% (many diffs)
7 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_001278686.2 62.7% 62.5% 0_1ins447;743C>T
8 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_001374396.1 62.7% 62.5% 0_1ins447;743C>T
9 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_001374397.1 62.7% 62.5% 0_1ins447;743C>T
10 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_170695.5 62.7% 62.5% 0_1ins447;743C>T
11 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_173209.3 62.7% 62.5% 0_1ins447;743C>T
12 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_173210.4 62.7% 62.5% 0_1ins447;743C>T
13 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_173211.2 62.7% 62.5% 0_1ins447;743C>T
14 human 7050 TGIF1 TGFB induced factor homeobox 1 NM_174886.3 62.7% 62.5% 0_1ins447;743C>T
15 mouse 21815 Tgif1 TGFB-induced factor homeobox 1 NM_001164075.1 64% 60% (many diffs)
16 mouse 21815 Tgif1 TGFB-induced factor homeobox 1 XM_011246363.2 60% 58.9% (many diffs)
17 mouse 21815 Tgif1 TGFB-induced factor homeobox 1 NM_009372.3 59.1% 59.8% (many diffs)
18 mouse 21815 Tgif1 TGFB-induced factor homeobox 1 XM_006524031.3 58.3% 59.8% (many diffs)
19 mouse 21815 Tgif1 TGFB-induced factor homeobox 1 NM_001164074.1 54.8% 56.6% (many diffs)
20 mouse 21815 Tgif1 TGFB-induced factor homeobox 1 NM_001164076.1 54.8% 56.6% (many diffs)
21 mouse 21815 Tgif1 TGFB-induced factor homeobox 1 NM_001164077.1 54.8% 56.6% (many diffs)
22 mouse 21815 Tgif1 TGFB-induced factor homeobox 1 XM_006524032.2 54.8% 56.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1269
ORF length:
1203
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt tctagcgcag agccgggtgt ctgccggggt gggctccccg cattgttcgg
121gctccggcgg gggcggctct gattcctttc catggcccgc ctcccacccc gggaatccgc
181agtgctcctt ttccacggct tttctggcgt ccccccgact ctcccgcggc actttggcct
241accttccccc agcgccgtgg tcctccctgg cgaccccctc tgcgctcctg gggtcctcct
301gcgccccccc tcctccaccg gcgcgctgcc cacagccgcg tgccctctcc caggagctgg
361ggaccaaggc tgggccccgc cggccgcatc ggtgggaact tccgcggtcc ccatcccagg
421gcgcacaggg tccagctcct cggcgccgac tcctggaaac aatgaaaggt attgttgcag
481catctggcag tgagactgag gatgaggaca gcatggacat tcccttggac ctttcttcat
541ccgctggctc aggcaagaga aggagaaggg gcaacctacc caaggagtct gtgcagattc
601ttcgggattg gctgtatgag caccgttaca atgcctatcc ttcagagcaa gaaaaagcgt
661tgctgtccca gcaaacacac ctgtctacgc tacaggtctg taactggttc atcaacgccc
721gccgcaggct cctccctgac atgctgagaa aggatggcaa agatccaaat cagttcacaa
781tttcccgccg tggggccaag atttctgaaa cgagctctgt ggagtccgtg atgggcatca
841aaaacttcat gccagctcta gaggagaccc catttcattc ctgtacagct gggccaaacc
901caaccctagg gaggccactg tctcctaagc cgtcatcccc gggatcagtt ttggctcgtc
961catcagtgat ctgccatacc actgtgactg cattgaaaga tgtccctttc tctctctgcc
1021agtcggtcgg tgtgggacaa aacacagata tacagcagat agcggccaaa aacttcacag
1081acacctctct catgtaccca gaggacactt gtaaatctgg accaagtacg aatacacaga
1141gtggtctttt caacactcct ccccctactc caccggacct caaccaggac ttcagtggat
1201ttcagcttct agtggatgtt gcactcaaac gggctgcaga gatggagctt caggtaaaac
1261ttacagctta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
1321tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
1381tcaacctctg gatta

Download FASTA (ORF) (Full)