Construct: ORF ccsbBroad304_07065
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012006.1_s304c1, BRDN0000462334
- Derived from:
- ccsbBroadEn_07065
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THBS2 (7058)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07065
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7058 | THBS2 | thrombospondin 2 | NM_003247.3 | 99.9% | 99.9% | 5T>C |
| 2 | mouse | 21826 | Thbs2 | thrombospondin 2 | NM_011581.3 | 83.8% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3585
- ORF length:
- 3516
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctggagg ctggtcctgc tggctctgtg ggtgtggccc agcacgcaag 121 ctggtcacca ggacaaagac acgaccttcg accttttcag tatcagcaac atcaaccgca 181 agaccattgg cgccaagcag ttccgcgggc ccgaccccgg cgtgccggct taccgcttcg 241 tgcgctttga ctacatccca ccggtgaacg cagatgacct cagcaagatc accaagatca 301 tgcggcagaa ggagggcttc ttcctcacgg cccagctcaa gcaggacggc aagtccaggg 361 gcacgctgtt ggctctggag ggccccggtc tctcccagag gcagttcgag atcgtctcca 421 acggccccgc ggacacgctg gatctcacct actggattga cggcacccgg catgtggtct 481 ccctggagga cgtcggcctg gctgactcgc agtggaagaa cgtcaccgtg caggtggctg 541 gcgagaccta cagcttgcac gtgggctgcg acctcataga cagcttcgct ctggacgagc 601 ccttctacga gcacctgcag gcggaaaaga gccggatgta cgtggccaaa ggctctgcca 661 gagagagtca cttcaggggt ttgcttcaga acgtccacct agtgtttgaa aactctgtgg 721 aagatattct aagcaagaag ggttgccagc aaggccaggg agctgagatc aacgccatca 781 gtgagaacac agagacgctg cgcctgggtc cgcatgtcac caccgagtac gtgggcccca 841 gctcggagag gaggcccgag gtgtgcgaac gctcgtgcga ggagctggga aacatggtcc 901 aggagctctc ggggctccac gtcctcgtga accagctcag cgagaacctc aagagagtgt 961 cgaatgataa ccagtttctc tgggagctca ttggtggccc tcctaagaca aggaacatgt 1021 cagcttgctg gcaggatggc cggttctttg cggaaaatga aacgtgggtg gtggacagct 1081 gcaccacgtg tacctgcaag aaatttaaaa ccatttgcca ccaaatcacc tgcccgcctg 1141 caacctgcgc cagtccatcc tttgtggaag gcgaatgctg cccttcctgc ctccactcgg 1201 tggacggtga ggagggctgg tctccgtggg cagagtggac ccagtgctcc gtgacgtgtg 1261 gctctgggac ccagcagaga ggccggtcct gtgacgtcac cagcaacacc tgcttggggc 1321 cctccatcca gacacgggct tgcagtctga gcaagtgtga cacccgcatc cggcaggacg 1381 gcggctggag ccactggtca ccttggtctt catgctctgt gacctgtgga gttggcaata 1441 tcacacgcat ccgtctctgc aactccccag tgccccagat ggggggcaag aattgcaaag 1501 ggagtggccg ggagaccaaa gcctgccagg gcgccccatg cccaatcgat ggccgctgga 1561 gcccctggtc cccgtggtcg gcctgcactg tcacctgtgc cggtgggatc cgggagcgca 1621 cccgggtctg caacagccct gagcctcagt acggagggaa ggcctgcgtg ggggatgtgc 1681 aggagcgtca gatgtgcaac aagaggagct gccccgtgga tggctgttta tccaacccct 1741 gcttcccggg agcccagtgc agcagcttcc ccgatgggtc ctggtcatgc ggctcctgcc 1801 ctgtgggctt cttgggcaat ggcacccact gtgaggacct ggacgagtgt gccctggtcc 1861 ccgacatctg cttctccacc agcaaggtgc ctcgctgtgt caacactcag cctggcttcc 1921 actgcctgcc ctgcccgccc cgatacagag ggaaccagcc cgtcggggtc ggcctggaag 1981 cagccaagac ggaaaagcaa gtgtgtgagc ccgaaaaccc atgcaaggac aagacacaca 2041 actgccacaa gcacgcggag tgcatctacc tgggccactt cagcgacccc atgtacaagt 2101 gcgagtgcca gacaggctac gcgggcgacg ggctcatctg cggggaggac tcggacctgg 2161 acggctggcc caacctcaat ctggtctgcg ccaccaacgc cacctaccac tgcatcaagg 2221 ataactgccc ccatctgcca aattctgggc aggaagactt tgacaaggac gggattggcg 2281 atgcctgtga tgatgacgat gacaatgacg gtgtgaccga tgagaaggac aactgccagc 2341 tcctcttcaa tccccgccag gctgactatg acaaggatga ggttggggac cgctgtgaca 2401 actgccctta cgtgcacaac cctgcccaga tcgacacaga caacaatgga gagggtgacg 2461 cctgctccgt ggacattgat ggggacgatg tcttcaatga acgagacaat tgtccctacg 2521 tctacaacac tgaccagagg gacacggatg gtgacggtgt gggggatcac tgtgacaact 2581 gccccctggt gcacaaccct gaccagaccg acgtggacaa tgaccttgtt ggggaccagt 2641 gtgacaacaa cgaggacata gatgacgacg gccaccagaa caaccaggac aactgcccct 2701 acatctccaa cgccaaccag gctgaccatg acagagacgg ccagggcgac gcctgtgacc 2761 ctgatgatga caacgatggc gtccccgatg acagggacaa ctgccggctt gtgttcaacc 2821 cagaccagga ggacttggac ggtgatggac ggggtgatat ttgtaaagat gattttgaca 2881 atgacaacat cccagatatt gatgatgtgt gtcctgaaaa caatgccatc agtgagacag 2941 acttcaggaa cttccagatg gtccccttgg atcccaaagg gaccacccaa attgatccca 3001 actgggtcat tcgccatcaa ggcaaggagc tggttcagac agccaactcg gaccccggca 3061 tcgctgtagg ttttgacgag tttgggtctg tggacttcag tggcacattc tacgtaaaca 3121 ctgaccggga cgacgactat gccggcttcg tctttggtta ccagtcaagc agccgcttct 3181 atgtggtgat gtggaagcag gtgacgcaga cctactggga ggaccagccc acgcgggcct 3241 atggctactc cggcgtgtcc ctcaaggtgg tgaactccac cacggggacg ggcgagcacc 3301 tgaggaacgc gctgtggcac acggggaaca cgccggggca ggtgcgaacc ttatggcacg 3361 accccaggaa cattggctgg aaggactaca cggcctatag gtggcacctg actcacaggc 3421 ccaagactgg ctacatcaga gtcttagtgc atgaaggaaa acaggtcatg gcagactcag 3481 gacctatcta tgaccaaacc tacgctggcg ggcggctggg tctatttgtc ttctctcaag 3541 aaatggtcta tttctcagac ctcaagtacg aatgcagaga tattttgcca actttcttgt 3601 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 3661 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a