Construct: ORF ccsbBroad304_07068

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014447.1_s304c1, BRDN0000403772
Derived from:
ccsbBroadEn_07068
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TIE1 (7075)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07068
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7075 TIE1 tyrosine kinase with immuno... NM_005424.5 99.9% 99.9% 321C>T;2334T>C;2381G>A
2 human 7075 TIE1 tyrosine kinase with immuno... XM_005271163.2 96.1% 96.1% (many diffs)
3 human 7075 TIE1 tyrosine kinase with immuno... NM_001253357.2 95.8% 95.7% (many diffs)
4 human 7075 TIE1 tyrosine kinase with immuno... XR_946751.2 85.1% (many diffs)
5 human 7075 TIE1 tyrosine kinase with immuno... XM_017002207.2 72.1% 70.5% (many diffs)
6 human 7075 TIE1 tyrosine kinase with immuno... XM_006710869.1 68.7% 68.7% 0_1ins1065;1269T>C;1316G>A
7 mouse 21846 Tie1 tyrosine kinase with immuno... NM_011587.2 87.3% 92.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3480
ORF length:
3414
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt ctggcgggtg ccccctttct tgctccccat cctcttcttg gcttctcatg
121tgggcgcggc ggtggacctg acgctgctgg ccaacctgcg gctcacggac ccccagcgct
181tcttcctgac ttgcgtgtct ggggaggccg gggcggggag gggctcggac gcctggggcc
241cgcccctgct gctggagaag gacgaccgta tcgtgcgcac cccgcccggg ccacccctgc
301gcctggcgcg caacggttcg caccaggtca cgcttcgcgg cttctccaag ccctcggacc
361tcgtgggcgt cttctcctgc gtgggtggtg ctggggcgcg gcgcacgcgc gtcatctacg
421tgcacaacag ccctggagcc cacctgcttc cagacaaggt cacacacact gtgaacaaag
481gtgacaccgc tgtactttct gcacgtgtgc acaaggagaa gcagacagac gtgatctgga
541agagcaacgg atcctacttc tacaccctgg actggcatga agcccaggat gggcggttcc
601tgctgcagct cccaaatgtg cagccaccat cgagcggcat ctacagtgcc acttacctgg
661aagccagccc cctgggcagc gccttctttc ggctcatcgt gcggggttgt ggggctgggc
721gctgggggcc aggctgtacc aaggagtgcc caggttgcct acatggaggt gtctgccacg
781accatgacgg cgaatgtgta tgcccccctg gcttcactgg cacccgctgt gaacaggcct
841gcagagaggg ccgttttggg cagagctgcc aggagcagtg cccaggcata tcaggctgcc
901ggggcctcac cttctgcctc ccagacccct atggctgctc ttgtggatct ggctggagag
961gaagccagtg ccaagaagct tgtgcccctg gtcattttgg ggctgattgc cgactccagt
1021gccagtgtca gaatggtggc acttgtgacc ggttcagtgg ttgtgtctgc ccctctgggt
1081ggcatggagt gcactgtgag aagtcagacc ggatccccca gatcctcaac atggcctcag
1141aactggagtt caacttagag acgatgcccc ggatcaactg tgcagctgca gggaacccct
1201tccccgtgcg gggcagcata gagctacgca agccagacgg cactgtgctc ctgtccacca
1261aggccattgt ggagccagag aagaccacag ctgagttcga ggtgccccgc ttggttcttg
1321cggacagtgg gttctgggag tgccgtgtgt ccacatctgg cggccaagac agccggcgct
1381tcaaggtcaa tgtgaaagtg ccccccgtgc ccctggctgc acctcggctc ctgaccaagc
1441agagccgcca gcttgtggtc tccccgctgg tctcgttctc tggggatgga cccatctcca
1501ctgtccgcct gcactaccgg ccccaggaca gtaccatgga ctggtcgacc attgtggtgg
1561accccagtga gaacgtgacg ttaatgaacc tgaggccaaa gacaggatac agtgttcgtg
1621tgcagctgag ccggccaggg gaaggaggag agggggcctg ggggcctccc accctcatga
1681ccacagactg tcctgagcct ttgttgcagc cgtggttgga gggctggcat gtggaaggca
1741ctgaccggct gcgagtgagc tggtccttgc ccttggtgcc cgggccactg gtgggcgacg
1801gtttcctgct gcgcctgtgg gacgggacac gggggcagga gcggcgggag aacgtctcat
1861ccccccaggc ccgcactgcc ctcctgacgg gactcacgcc tggcacccac taccagctgg
1921atgtgcagct ctaccactgc accctcctgg gcccggcctc gccccctgca cacgtgcttc
1981tgccccccag tgggcctcca gccccccgac acctccacgc ccaggccctc tcagactccg
2041agatccagct gacatggaag cacccggagg ctctgcctgg gccaatatcc aagtacgttg
2101tggaggtgca ggtggctggg ggtgcaggag acccactgtg gatagacgtg gacaggcctg
2161aggagacaag caccatcatc cgtggcctca acgccagcac gcgctacctc ttccgcatgc
2221gggccagcat tcaggggctc ggggactgga gcaacacagt agaagagtcc accctgggca
2281acgggctgca ggctgagggc ccagtccaag agagccgggc agctgaagag ggcctggatc
2341agcagctgat cctggcggtg gtgggctccg tgtctgccac ctgcctcacc atcctggccg
2401cccttttaac cctggtgtgc atccgcagaa gctgcctgca tcggaaacgc accttcacct
2461accagtcagg ctcgggcgag gagaccatcc tgcagttcag ctcagggacc ttgacactta
2521cccggcggcc aaaactgcag cccgagcccc tgagctaccc agtgctagag tgggaggaca
2581tcacctttga ggacctcatc ggggagggga acttcggcca ggtcatccgg gccatgatca
2641agaaggacgg gctgaagatg aacgcagcca tcaaaatgct gaaagagtat gcctctgaaa
2701atgaccatcg tgactttgcg ggagaactgg aagttctgtg caaattgggg catcacccca
2761acatcatcaa cctcctgggg gcctgtaaga accgaggtta cttgtatatc gctattgaat
2821atgcccccta cgggaacctg ctagattttc tgcggaaaag ccgggtccta gagactgacc
2881cagcttttgc tcgagagcat gggacagcct ctacccttag ctcccggcag ctgctgcgtt
2941tcgccagtga tgcggccaat ggcatgcagt acctgagtga gaagcagttc atccacaggg
3001acctggctgc ccggaatgtg ctggtcggag agaacctagc ctccaagatt gcagacttcg
3061gcctttctcg gggagaggag gtttatgtga agaagacgat ggggcgtctc cctgtgcgct
3121ggatggccat tgagtccctg aactacagtg tctataccac caagagtgat gtctggtcct
3181ttggagtcct tctttgggag atagtgagcc ttggaggtac accctactgt ggcatgacct
3241gtgccgagct ctatgaaaag ctgccccagg gctaccgcat ggagcagcct cgaaactgtg
3301acgatgaagt gtacgagctg atgcgtcagt gctggcggga ccgtccctat gagcgacccc
3361cctttgccca gattgcgcta cagctaggcc gcatgctgga agccaggaag gcctatgtga
3421acatgtcgct gtttgagaac ttcacttacg cgggcattga tgccacagct gaggaggcct
3481gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
3541attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
3601ggatta

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