Construct: ORF ccsbBroad304_07068
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014447.1_s304c1, BRDN0000403772
- Derived from:
- ccsbBroadEn_07068
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIE1 (7075)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07068
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7075 | TIE1 | tyrosine kinase with immuno... | NM_005424.5 | 99.9% | 99.9% | 321C>T;2334T>C;2381G>A |
| 2 | human | 7075 | TIE1 | tyrosine kinase with immuno... | XM_005271163.2 | 96.1% | 96.1% | (many diffs) |
| 3 | human | 7075 | TIE1 | tyrosine kinase with immuno... | NM_001253357.2 | 95.8% | 95.7% | (many diffs) |
| 4 | human | 7075 | TIE1 | tyrosine kinase with immuno... | XR_946751.2 | 85.1% | (many diffs) | |
| 5 | human | 7075 | TIE1 | tyrosine kinase with immuno... | XM_017002207.2 | 72.1% | 70.5% | (many diffs) |
| 6 | human | 7075 | TIE1 | tyrosine kinase with immuno... | XM_006710869.1 | 68.7% | 68.7% | 0_1ins1065;1269T>C;1316G>A |
| 7 | mouse | 21846 | Tie1 | tyrosine kinase with immuno... | NM_011587.2 | 87.3% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3480
- ORF length:
- 3414
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ctggcgggtg ccccctttct tgctccccat cctcttcttg gcttctcatg 121 tgggcgcggc ggtggacctg acgctgctgg ccaacctgcg gctcacggac ccccagcgct 181 tcttcctgac ttgcgtgtct ggggaggccg gggcggggag gggctcggac gcctggggcc 241 cgcccctgct gctggagaag gacgaccgta tcgtgcgcac cccgcccggg ccacccctgc 301 gcctggcgcg caacggttcg caccaggtca cgcttcgcgg cttctccaag ccctcggacc 361 tcgtgggcgt cttctcctgc gtgggtggtg ctggggcgcg gcgcacgcgc gtcatctacg 421 tgcacaacag ccctggagcc cacctgcttc cagacaaggt cacacacact gtgaacaaag 481 gtgacaccgc tgtactttct gcacgtgtgc acaaggagaa gcagacagac gtgatctgga 541 agagcaacgg atcctacttc tacaccctgg actggcatga agcccaggat gggcggttcc 601 tgctgcagct cccaaatgtg cagccaccat cgagcggcat ctacagtgcc acttacctgg 661 aagccagccc cctgggcagc gccttctttc ggctcatcgt gcggggttgt ggggctgggc 721 gctgggggcc aggctgtacc aaggagtgcc caggttgcct acatggaggt gtctgccacg 781 accatgacgg cgaatgtgta tgcccccctg gcttcactgg cacccgctgt gaacaggcct 841 gcagagaggg ccgttttggg cagagctgcc aggagcagtg cccaggcata tcaggctgcc 901 ggggcctcac cttctgcctc ccagacccct atggctgctc ttgtggatct ggctggagag 961 gaagccagtg ccaagaagct tgtgcccctg gtcattttgg ggctgattgc cgactccagt 1021 gccagtgtca gaatggtggc acttgtgacc ggttcagtgg ttgtgtctgc ccctctgggt 1081 ggcatggagt gcactgtgag aagtcagacc ggatccccca gatcctcaac atggcctcag 1141 aactggagtt caacttagag acgatgcccc ggatcaactg tgcagctgca gggaacccct 1201 tccccgtgcg gggcagcata gagctacgca agccagacgg cactgtgctc ctgtccacca 1261 aggccattgt ggagccagag aagaccacag ctgagttcga ggtgccccgc ttggttcttg 1321 cggacagtgg gttctgggag tgccgtgtgt ccacatctgg cggccaagac agccggcgct 1381 tcaaggtcaa tgtgaaagtg ccccccgtgc ccctggctgc acctcggctc ctgaccaagc 1441 agagccgcca gcttgtggtc tccccgctgg tctcgttctc tggggatgga cccatctcca 1501 ctgtccgcct gcactaccgg ccccaggaca gtaccatgga ctggtcgacc attgtggtgg 1561 accccagtga gaacgtgacg ttaatgaacc tgaggccaaa gacaggatac agtgttcgtg 1621 tgcagctgag ccggccaggg gaaggaggag agggggcctg ggggcctccc accctcatga 1681 ccacagactg tcctgagcct ttgttgcagc cgtggttgga gggctggcat gtggaaggca 1741 ctgaccggct gcgagtgagc tggtccttgc ccttggtgcc cgggccactg gtgggcgacg 1801 gtttcctgct gcgcctgtgg gacgggacac gggggcagga gcggcgggag aacgtctcat 1861 ccccccaggc ccgcactgcc ctcctgacgg gactcacgcc tggcacccac taccagctgg 1921 atgtgcagct ctaccactgc accctcctgg gcccggcctc gccccctgca cacgtgcttc 1981 tgccccccag tgggcctcca gccccccgac acctccacgc ccaggccctc tcagactccg 2041 agatccagct gacatggaag cacccggagg ctctgcctgg gccaatatcc aagtacgttg 2101 tggaggtgca ggtggctggg ggtgcaggag acccactgtg gatagacgtg gacaggcctg 2161 aggagacaag caccatcatc cgtggcctca acgccagcac gcgctacctc ttccgcatgc 2221 gggccagcat tcaggggctc ggggactgga gcaacacagt agaagagtcc accctgggca 2281 acgggctgca ggctgagggc ccagtccaag agagccgggc agctgaagag ggcctggatc 2341 agcagctgat cctggcggtg gtgggctccg tgtctgccac ctgcctcacc atcctggccg 2401 cccttttaac cctggtgtgc atccgcagaa gctgcctgca tcggaaacgc accttcacct 2461 accagtcagg ctcgggcgag gagaccatcc tgcagttcag ctcagggacc ttgacactta 2521 cccggcggcc aaaactgcag cccgagcccc tgagctaccc agtgctagag tgggaggaca 2581 tcacctttga ggacctcatc ggggagggga acttcggcca ggtcatccgg gccatgatca 2641 agaaggacgg gctgaagatg aacgcagcca tcaaaatgct gaaagagtat gcctctgaaa 2701 atgaccatcg tgactttgcg ggagaactgg aagttctgtg caaattgggg catcacccca 2761 acatcatcaa cctcctgggg gcctgtaaga accgaggtta cttgtatatc gctattgaat 2821 atgcccccta cgggaacctg ctagattttc tgcggaaaag ccgggtccta gagactgacc 2881 cagcttttgc tcgagagcat gggacagcct ctacccttag ctcccggcag ctgctgcgtt 2941 tcgccagtga tgcggccaat ggcatgcagt acctgagtga gaagcagttc atccacaggg 3001 acctggctgc ccggaatgtg ctggtcggag agaacctagc ctccaagatt gcagacttcg 3061 gcctttctcg gggagaggag gtttatgtga agaagacgat ggggcgtctc cctgtgcgct 3121 ggatggccat tgagtccctg aactacagtg tctataccac caagagtgat gtctggtcct 3181 ttggagtcct tctttgggag atagtgagcc ttggaggtac accctactgt ggcatgacct 3241 gtgccgagct ctatgaaaag ctgccccagg gctaccgcat ggagcagcct cgaaactgtg 3301 acgatgaagt gtacgagctg atgcgtcagt gctggcggga ccgtccctat gagcgacccc 3361 cctttgccca gattgcgcta cagctaggcc gcatgctgga agccaggaag gcctatgtga 3421 acatgtcgct gtttgagaac ttcacttacg cgggcattga tgccacagct gaggaggcct 3481 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 3541 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 3601 ggatta