Transcript: Human NM_001253357.2

Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-23
Taxon:
Homo sapiens (human)
Gene:
TIE1 (7075)
Length:
3810
CDS:
143..3424

Additional Resources:

NCBI RefSeq record:
NM_001253357.2
NBCI Gene record:
TIE1 (7075)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147382 AGACCCACTGTGGATAGACG pXPR_003 TGG 1945 59% 13 0.3612 TIE1 TIE1 76686
2 BRDN0001147476 CTGTCCGCAAGAACCAAGCG pXPR_003 GGG 1110 34% 9 0.3539 TIE1 TIE1 76688
3 BRDN0001144893 ACGTGACGTTAATGAACCTG pXPR_003 AGG 1389 42% 11 0.2655 TIE1 TIE1 76687
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001253357.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000379802 AGTGAGAACGTGACGTTAATG pLKO_005 1508 CDS 100% 13.200 18.480 N TIE1 n/a
2 TRCN0000121189 CTACGGGAACCTGCTAGATTT pLKO.1 2770 CDS 100% 13.200 18.480 N TIE1 n/a
3 TRCN0000121116 GAACCGAGGTTACTTGTATAT pLKO.1 2731 CDS 100% 13.200 18.480 N TIE1 n/a
4 TRCN0000381103 CCATGACGGCGAATGTGTATG pLKO_005 724 CDS 100% 10.800 15.120 N TIE1 n/a
5 TRCN0000121115 CCTCGAAACTGTGACGATGAA pLKO.1 3230 CDS 100% 4.950 6.930 N TIE1 n/a
6 TRCN0000121269 GCCAATATCCAAGTACGTTGT pLKO.1 2023 CDS 100% 4.050 5.670 N TIE1 n/a
7 TRCN0000199514 GCGCCTTCTTTCGGCTCATCG pLKO.1 621 CDS 100% 0.000 0.000 N TIE1 n/a
8 TRCN0000121188 GACTGGAGCAACACAGTAGAA pLKO.1 2186 CDS 100% 4.950 3.960 N TIE1 n/a
9 TRCN0000199821 GTGGCACTTGTGACCGGTTCA pLKO.1 978 CDS 100% 1.350 1.080 N TIE1 n/a
10 TRCN0000310162 ACTGGAAGTTCTGTGCAAATT pLKO_005 2668 CDS 100% 13.200 9.240 N TIE1 n/a
11 TRCN0000121113 CCCTGAACTACAGTGTCTATA pLKO.1 3078 CDS 100% 13.200 9.240 N TIE1 n/a
12 TRCN0000307973 AGGTGACACCGCTGTACTTTC pLKO_005 421 CDS 100% 10.800 7.560 N TIE1 n/a
13 TRCN0000381467 ATGTGAACATGTCGCTGTTTG pLKO_005 3357 CDS 100% 10.800 7.560 N TIE1 n/a
14 TRCN0000310098 CAGAACTGGAGTTCAACTTAG pLKO_005 1080 CDS 100% 10.800 7.560 N TIE1 n/a
15 TRCN0000295866 CTCAGGGACCTTGACACTTAC pLKO_005 2443 CDS 100% 10.800 7.560 N TIE1 n/a
16 TRCN0000196450 GTTACTTGTATATCGCTATTG pLKO.1 2739 CDS 100% 10.800 7.560 N TIE1 n/a
17 TRCN0000381984 CCTACGGGAACCTGCTAGATT pLKO_005 2769 CDS 100% 5.625 3.938 N TIE1 n/a
18 TRCN0000121191 CCTGAGTGAGAAGCAGTTCAT pLKO.1 2914 CDS 100% 4.950 3.465 N TIE1 n/a
19 TRCN0000121187 CGGAGCAAACTCTGCTGTCTA pLKO.1 3458 3UTR 100% 4.950 3.465 N TIE1 n/a
20 TRCN0000001606 TGAGGCCAAAGACAGGATACA pLKO.1 1533 CDS 100% 4.950 3.465 N TIE1 n/a
21 TRCN0000001603 CGATGAAGTGTACGAGCTGAT pLKO.1 3244 CDS 100% 4.050 2.835 N TIE1 n/a
22 TRCN0000121271 CTATGTGAACATGTCGCTGTT pLKO.1 3355 CDS 100% 4.050 2.835 N TIE1 n/a
23 TRCN0000121267 CTCTGACTTAAGCTGCCTCAA pLKO.1 3506 3UTR 100% 4.050 2.835 N TIE1 n/a
24 TRCN0000288650 CTCTGACTTAAGCTGCCTCAA pLKO_005 3506 3UTR 100% 4.050 2.835 N TIE1 n/a
25 TRCN0000001604 CTTTGGGAGATAGTGAGCCTT pLKO.1 3134 CDS 100% 2.640 1.848 N TIE1 n/a
26 TRCN0000199921 GCTGAAAGAGTATGCCTCTGA pLKO.1 2620 CDS 100% 2.640 1.848 N TIE1 n/a
27 TRCN0000121270 GCCTGAGGAGACAAGCACCAT pLKO.1 2098 CDS 100% 0.880 0.616 N TIE1 n/a
28 TRCN0000121268 GCCACGACCATGACGGCGAAT pLKO.1 717 CDS 100% 0.000 0.000 N TIE1 n/a
29 TRCN0000121114 CCAGTGAGAACGTGACGTTAA pLKO.1 1506 CDS 100% 10.800 6.480 N TIE1 n/a
30 TRCN0000001602 CAGCACTCACACCACTAACAT pLKO.1 3723 3UTR 100% 5.625 3.375 N TIE1 n/a
31 TRCN0000001605 AGAGGAGGTTTATGTGAAGAA pLKO.1 3016 CDS 100% 4.950 2.970 N TIE1 n/a
32 TRCN0000121112 CATGCTTTGTAGGTGTCTCAT pLKO.1 3597 3UTR 100% 4.950 2.970 N TIE1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001253357.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14864 pDONR223 0% 95.9% 95.8% None (many diffs) n/a
2 ccsbBroad304_14864 pLX_304 0% 95.9% 95.8% V5 (many diffs) n/a
3 ccsbBroadEn_07068 pDONR223 100% 95.8% 95.7% None (many diffs) n/a
4 ccsbBroad304_07068 pLX_304 0% 95.8% 95.7% V5 (many diffs) n/a
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