Construct: ORF ccsbBroad304_07102

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017055.1_s304c1, BRDN0000409446
Derived from:
ccsbBroadEn_07102
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TSG101 (7251)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07102
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7251 TSG101 tumor susceptibility 101 NM_006292.4 99.9% 99.7% 1027T>C
2 human 7251 TSG101 tumor susceptibility 101 XM_005253108.4 86.5% 86.4% 0_1ins156;871T>C
3 mouse 22088 Tsg101 tumor susceptibility gene 101 NM_021884.4 89.4% 94.3% (many diffs)
4 mouse 22088 Tsg101 tumor susceptibility gene 101 XM_006540788.3 80.9% 85.6% (many diffs)
5 mouse 22088 Tsg101 tumor susceptibility gene 101 NM_001348088.1 77% 81.3% (many diffs)
6 mouse 22088 Tsg101 tumor susceptibility gene 101 XM_006540789.3 77% 81.3% (many diffs)
7 mouse 22088 Tsg101 tumor susceptibility gene 101 NM_001348089.1 67.8% 72.1% (many diffs)
8 mouse 22088 Tsg101 tumor susceptibility gene 101 XM_006540790.3 67.8% 72.1% (many diffs)
9 mouse 22088 Tsg101 tumor susceptibility gene 101 XR_391333.3 61% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1236
ORF length:
1170
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggtgtcggag agccagctca agaaaatggt gtccaagtac aaatacagag
121acctaactgt acgtgaaact gtcaatgtta ttactctata caaagatctc aaacctgttt
181tggattcata tgtttttaac gatggcagtt ccagggaact aatgaacctc actggaacaa
241tccctgtgcc ttatagaggt aatacataca atattccaat atgcctatgg ctactggaca
301catacccata taatccccct atctgttttg ttaagcctac tagttcaatg actattaaaa
361caggaaagca tgttgatgca aatgggaaga tatatcttcc ttatctacat gaatggaaac
421acccacagtc agacttgttg gggcttattc aggtcatgat tgtggtattt ggagatgaac
481ctccagtctt ctctcgtcct atttcggcat cctatccgcc ataccaggca acggggccac
541caaatacttc ctacatgcca ggcatgccag gtggaatctc tccataccca tccggatacc
601ctcccaatcc cagtggttac ccaggctgtc cttacccacc tggtggtcca tatcctgcca
661caacaagttc tcagtaccct tctcagcctc ctgtgaccac tgttggtccc agtagggatg
721gcacaatcag cgaggacacc atccgagcct ctctcatctc tgcggtcagt gacaaactga
781gatggcggat gaaggaggaa atggatcgtg cccaggcaga gctcaatgcc ttgaaacgaa
841cagaagaaga cctgaaaaag ggtcaccaga aactggaaga gatggttacc cgtttagatc
901aagaagtagc cgaggttgat aaaaacatag aacttttgaa aaagaaggat gaagaactca
961gttctgctct ggaaaaaatg gaaaatcagt ctgaaaacaa tgatatcgat gaagttatca
1021ttcccacagc tcccttatac aaacagatcc tgaatctgta tgcagaagaa aacgctattg
1081aagacactat cctttacttg ggagaagcct tgagaagggg cgtgatagac ctggatgtct
1141tcctgaagca tgtacgtctt ctgtcccgta aacagttcca gctgagggca ctaatgcaaa
1201aagcaagaaa gactgccggt ctcagtgacc tctactgccc aactttcttg tacaaagtgg
1261ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1321ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)