Transcript: Human NM_006292.4

Homo sapiens tumor susceptibility 101 (TSG101), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
TSG101 (7251)
Length:
1534
CDS:
127..1299

Additional Resources:

NCBI RefSeq record:
NM_006292.4
NBCI Gene record:
TSG101 (7251)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006292.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000315112 GTACGTCTTCTGTCCCGTAAA pLKO_005 1213 CDS 100% 10.800 15.120 N TSG101 n/a
2 TRCN0000007563 GCCTTATAGAGGTAATACATA pLKO.1 309 CDS 100% 5.625 7.875 N TSG101 n/a
3 TRCN0000315109 GCCTTATAGAGGTAATACATA pLKO_005 309 CDS 100% 5.625 7.875 N TSG101 n/a
4 TRCN0000007561 CGTCCTATTTCGGCATCCTAT pLKO.1 556 CDS 100% 4.950 6.930 N TSG101 n/a
5 TRCN0000315042 ACTGGACACATACCCATATAA pLKO_005 354 CDS 100% 15.000 10.500 N TSG101 n/a
6 TRCN0000380512 AGAAGTAGCCGAGGTTGATAA pLKO_005 963 CDS 100% 13.200 9.240 N TSG101 n/a
7 TRCN0000379499 CAGTCTTCTCTCGTCCTATTT pLKO_005 545 CDS 100% 13.200 9.240 N TSG101 n/a
8 TRCN0000380124 TGGAGGTTGAGCTCTTCTTAA pLKO_005 1316 3UTR 100% 13.200 9.240 N TSG101 n/a
9 TRCN0000380902 AGACCTAACTGTACGTGAAAC pLKO_005 180 CDS 100% 10.800 7.560 N TSG101 n/a
10 TRCN0000007564 GCAGAGCTCAATGCCTTGAAA pLKO.1 877 CDS 100% 5.625 3.938 N TSG101 n/a
11 TRCN0000315110 GCAGAGCTCAATGCCTTGAAA pLKO_005 877 CDS 100% 5.625 3.938 N TSG101 n/a
12 TRCN0000054606 GCTATTGAAGACACTATCTTT pLKO.1 1135 CDS 100% 5.625 3.938 N Tsg101 n/a
13 TRCN0000007562 CCTTATCTACATGAATGGAAA pLKO.1 460 CDS 100% 4.950 3.465 N TSG101 n/a
14 TRCN0000007560 CCCAGAATAAGTTATTGCAGT pLKO.1 1368 3UTR 100% 2.640 1.848 N TSG101 n/a
15 TRCN0000054603 CCCGTAAACAGTTCCAGCTAA pLKO.1 1226 CDS 100% 4.950 3.465 N Tsg101 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006292.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07102 pDONR223 100% 99.9% 99.7% None 1027T>C n/a
2 ccsbBroad304_07102 pLX_304 0% 99.9% 99.7% V5 1027T>C n/a
3 TRCN0000467414 AACTTTTCCTCGATACTAAAAATC pLX_317 33.1% 99.9% 99.7% V5 1027T>C n/a
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