Construct: ORF ccsbBroad304_07113

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010759.1_s304c1, BRDN0000404000
Derived from:
ccsbBroadEn_07113
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
U2AF1 (7307)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07113
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7307 U2AF1 U2 small nuclear RNA auxili... NM_001025204.1 99.8% 99.4% 61G>A
2 human 102724594 U2AF1L5 U2 small nuclear RNA auxili... NM_001320651.1 99.8% 99.4% 61G>A
3 human 102724594 U2AF1L5 U2 small nuclear RNA auxili... XM_024452037.1 99.8% 99.4% 61G>A
4 human 102724594 U2AF1L5 U2 small nuclear RNA auxili... XM_024452038.1 99.8% 99.4% 61G>A
5 human 102724594 U2AF1L5 U2 small nuclear RNA auxili... XM_024452039.1 99.8% 99.4% 61G>A
6 human 7307 U2AF1 U2 small nuclear RNA auxili... XM_024452129.1 99.8% 99.4% 61G>A
7 human 7307 U2AF1 U2 small nuclear RNA auxili... XM_024452130.1 99.8% 99.4% 61G>A
8 human 7307 U2AF1 U2 small nuclear RNA auxili... XM_024452131.1 99.8% 99.4% 61G>A
9 human 102724594 U2AF1L5 U2 small nuclear RNA auxili... NM_001320650.2 82.5% 82.1% 1_105del;166G>A
10 human 102724594 U2AF1L5 U2 small nuclear RNA auxili... XM_017028220.1 73.3% 55.8% (many diffs)
11 human 7307 U2AF1 U2 small nuclear RNA auxili... XM_017028468.2 73.3% 55.8% (many diffs)
12 human 7307 U2AF1 U2 small nuclear RNA auxili... XM_011529743.3 71.6% 55.8% (many diffs)
13 human 102724594 U2AF1L5 U2 small nuclear RNA auxili... XM_017028219.1 71.6% 55.8% (many diffs)
14 human 7307 U2AF1 U2 small nuclear RNA auxili... NM_001025203.1 69.4% 69.1% 1_219del;280G>A
15 human 102724594 U2AF1L5 U2 small nuclear RNA auxili... NM_001320646.2 69.4% 69.1% 1_219del;280G>A
16 human 102724594 U2AF1L5 U2 small nuclear RNA auxili... NM_001320648.2 69.4% 69.1% 1_219del;280G>A
17 human 7307 U2AF1 U2 small nuclear RNA auxili... NM_006758.2 69.4% 69.1% 1_219del;280G>A
18 mouse 108121 U2af1 U2 small nuclear ribonucleo... XM_006523472.1 87.6% 98.8% (many diffs)
19 mouse 108121 U2af1 U2 small nuclear ribonucleo... XM_006523473.2 87.6% 98.8% (many diffs)
20 mouse 108121 U2af1 U2 small nuclear ribonucleo... NM_001163769.1 60.9% 68.7% (many diffs)
21 mouse 108121 U2af1 U2 small nuclear ribonucleo... NM_024187.4 60.9% 68.7% (many diffs)
22 mouse 629480 Gm6978 predicted gene 6978 XR_386288.3 50.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
567
ORF length:
501
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca ggaacactat gatgagtttt ttgaggaggt ttttacagaa atggaggaga
121agtataggga agtagaggag atgaacgtct gtgacaacct gggagaccac ctggtgggga
181acgtgtacgt caagtttcgc cgtgaggaag atgcggaaaa ggctgtgatt gacttgaata
241accgttggtt taatggacag ccgatccacg ccgagctgtc acccgtgacg gacttcagag
301aagcctgctg ccgtcagtat gagatgggag aatgcacacg aggcggcttc tgcaacttca
361tgcatttgaa gcccatttcc agagagctgc ggcgggagct gtatggccgc cgtcgcaaga
421agcatagatc aagatcccga tcccgggagc gtcgttctcg gtctagagac cgtggtcgtg
481gcggtggcgg tggcggtggt ggaggtggcg gcggacggga gcgtgacagg aggcggtcga
541gagatcgtga aagatctggg cgattctgcc caactttctt gtacaaagtg gttggtaagc
601ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
661ggcgcgttaa gtcgacaatc aacctctgga tta

Download FASTA (ORF) (Full)