Construct: ORF ccsbBroad304_07120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005371.1_s304c1, BRDN0000462375
- Derived from:
- ccsbBroadEn_07120
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP4 (7375)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07120
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7375 | USP4 | ubiquitin specific peptidase 4 | NM_003363.4 | 99.9% | 100% | 591G>A;1101C>T |
| 2 | human | 7375 | USP4 | ubiquitin specific peptidase 4 | NM_199443.3 | 95% | 95% | 591G>A;695_696ins141;960C>T |
| 3 | human | 7375 | USP4 | ubiquitin specific peptidase 4 | NM_001251877.2 | 29.8% | 26.1% | (many diffs) |
| 4 | mouse | 22258 | Usp4 | ubiquitin specific peptidas... | NM_011678.2 | 87.7% | 90.7% | (many diffs) |
| 5 | mouse | 22258 | Usp4 | ubiquitin specific peptidas... | XM_006511696.2 | 87.3% | 90.1% | (many diffs) |
| 6 | mouse | 22258 | Usp4 | ubiquitin specific peptidas... | NM_001311157.1 | 83.2% | 86.3% | (many diffs) |
| 7 | mouse | 22258 | Usp4 | ubiquitin specific peptidas... | XM_006511697.2 | 82.8% | 85.8% | (many diffs) |
| 8 | mouse | 22258 | Usp4 | ubiquitin specific peptidas... | XM_006511700.3 | 79.2% | 81.1% | (many diffs) |
| 9 | mouse | 22258 | Usp4 | ubiquitin specific peptidas... | XM_006511699.4 | 78.7% | 80.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2958
- ORF length:
- 2889
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggaaggt ggaggctgcc gtgagcgacc ggatgcggag actcagaagt 121 ccgagcttgg acccttaatg aggaccacac tccaacgcgg ggcgcagtgg tatcttattg 181 acagccggtg gttcaagcag tggaagaagt atgtgggctt tgacagctgg gacatgtaca 241 atgtgggtga acataaccta tttcctggcc caatagacaa ctctgggcta ttttcagatc 301 ctgagagtca gaccttgaaa gaacacttaa ttgatgaatt ggactatgta ttggtcccta 361 ccgaggcgtg gaataaacta ctaaactggt acggctgtgt agaaggccag caacccatcg 421 tcagaaaagt tgtggagcat ggcctgtttg tcaagcactg caaagtcgag gtgtatttgc 481 tggaactgaa gctctgtgag aacagtgacc ccaccaatgt gctgagttgc catttcagca 541 aggcagacac cattgcaacc atcgagaaag agatgcggaa gctattcaac atccctgcgg 601 agcgtgaaac acggctctgg aacaaataca tgagcaacac ctacgagcag ttgagcaaac 661 tagacaacac tgtccaggat gctgggctat accagggtca ggtgctagta attgagcctc 721 aaaatgaaga tggcacatgg cccaggcaga ccttgcagtc aaaatcaagc actgcgccta 781 gcagaaattt tactacctct ccaaaatcat cagcaagtcc ctattcctca gtgtctgcct 841 ctctcattgc aaatggtgat agcactagca cctgtgggat gcacagttcc ggtgtcagca 901 ggggtggatc tggcttttct gcttcgtata attgtcagga gccaccatcc tctcatatac 961 aacctgggct ctgtggactt ggaaacctgg gaaacacctg cttcatgaac tccgctttgc 1021 agtgtttgag caacactgca ccactgactg actactttct caaagatgag tatgaagccg 1081 aaatcaacag agacaaccct ctggggatga aaggggaaat tgcagaagcc tatgctgaac 1141 tcattaagca gatgtggtct ggaagggatg cccatgtggc acctcgcatg ttcaaaactc 1201 aagtaggacg ttttgctcct caattttctg gctaccagca acaagattct caggagctgc 1261 tggcctttct tctagatgga ttgcatgaag atctgaaccg ggtaaagaaa aagccctact 1321 tggagctgaa ggatgccaat gggcggccag atgcggtggt ggcaaaggaa gcctgggaga 1381 atcacaggtt gaggaatgat tctgtgattg tggatacttt ccatggcctc ttcaaatcta 1441 ctttggtttg cccagaatgt gctaaggttt ctgtgacctt tgacccattt tgctatctaa 1501 cgctgccact gcccttgaag aaagatcgag ttatggaggt tttcctggtt cctgctgacc 1561 ctcactgcag acctactcag taccgtgtga ctgtgccgct gatgggggct gtgtccgacc 1621 tgtgcgaggc tctctccagg ctgtctggca ttgctgcaga aaatatggtg gtcgcagatg 1681 tgtataatca ccgattccac aaaattttcc aaatggatga aggtttaaac cacatcatgc 1741 ctcgggatga cattttcgtg tacgaggtct gcagcacttc cgtggatggc tcggaatgtg 1801 tcacgcttcc agtctacttc agggagagga agtccaggcc atcaagcact tcctccgcat 1861 cagcgctata tgggcagcca ctattgcttt ctgtccccaa gcacaagtta acccttgagt 1921 ctttgtacca ggctgtttgt gatcgtatca gccgctatgt gaaacagcct ttacctgatg 1981 agtttggcag ctcacccttg gagccagggg cctgcaatgg ctccaggaac agctgtgaag 2041 gagaagatga ggaagaaatg gagcatcagg aagaaggcaa agagcagctt tcagaaacag 2101 aaggcagtgg ggaagatgag ccaggaaatg accccagtga gaccacccaa aagaagatca 2161 aaggccagcc ctgcccaaaa aggcttttta ccttcagtct tgtgaactcc tatggaacag 2221 ctgacataaa ttcacttgca gctgatggaa aactacttaa actcaactct cgatctacac 2281 tggccatgga ttgggacagt gaaactcgga gactttacta tgatgagcaa gaatctgagg 2341 cctacgagaa gcatgtgagc atgttgcagc ctcagaagaa gaagaagacc acagtggccc 2401 tgagagactg catcgagctc ttcaccacca tggagaccct tggggagcat gacccctggt 2461 actgtcccaa ctgtaagaag catcaacagg ccacaaaaaa gtttgaccta tggtccttgc 2521 ccaagatcct ggtggtccac ctcaaacgtt tctcctacaa cagatactgg agggataagc 2581 tcgacacagt cgtagaattc ccaatcagag ggctgaacat gtccgagttt gtctgtaacc 2641 tgtcagcaag gccttatgtg tacgacctca ttgccgtgtc caatcattat ggagccatgg 2701 gggttggcca ctacactgca tatgcgaaga acaaactgaa tggtaaatgg tattactttg 2761 atgatagcaa cgtgtccctg gcctctgagg atcagatagt gactaaagca gcttatgtgc 2821 tattttacca acgtcgagat gatgaatttt ataagacacc ttcacttagc agttctggtt 2881 cctctgatgg agggacacga ccaagcagct ctcagcaggg ctttggggat gatgaggctt 2941 gcagcatgga caccaacttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 3001 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 3061 agtcgacaat caacctctgg atta