Construct: ORF ccsbBroad304_07126
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007052.1_s304c1, BRDN0000403755
- Derived from:
- ccsbBroadEn_07126
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VARS1 (7407)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07126
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7407 | VARS1 | valyl-tRNA synthetase 1 | NM_006295.3 | 99.9% | 99.9% | 151_152delCCinsAG;1335C>T |
| 2 | human | 7407 | VARS1 | valyl-tRNA synthetase 1 | XM_005249362.2 | 99.8% | 99.8% | 151_152delCCinsAG;785_787delAGC;1338C>T |
| 3 | human | 7407 | VARS1 | valyl-tRNA synthetase 1 | XR_926304.2 | 71.3% | (many diffs) | |
| 4 | human | 7407 | VARS1 | valyl-tRNA synthetase 1 | XM_017011246.1 | 53.9% | 53.9% | 0_1ins1746 |
| 5 | human | 7407 | VARS1 | valyl-tRNA synthetase 1 | XM_024446537.1 | 53.9% | 53.9% | 0_1ins1746 |
| 6 | mouse | 22321 | Vars | valyl-tRNA synthetase | NM_011690.3 | 87.7% | 93.3% | (many diffs) |
| 7 | mouse | 22321 | Vars | valyl-tRNA synthetase | XM_017317395.1 | 87.7% | 93.3% | (many diffs) |
| 8 | mouse | 22321 | Vars | valyl-tRNA synthetase | XM_017317396.1 | 62.4% | 67% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3858
- ORF length:
- 3792
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc caccctctac gtctcccctc acccagatgc cttccccagc ctccgagccc 121 tcatagccgc tcgctatggg gaggctgggg agggtcccgg atggggagga gcccaccccc 181 gcatctgtct ccagccaccc ccgactagca ggactagctt tcccccaccc cgcctgccgg 241 ccctggagca ggggcccggt gggctctggg tgtggggggc cacggctgtg gcccagctgc 301 tgtggccagc aggcctgggg ggcccagggg gcagccgggc ggctgtcctt gtccaacagt 361 gggtcagtta cgccgacacg gagttaatac cagctgcctg tggagcaacg ctgccggccc 421 tgggactccg aagctcggcc caggaccccc aggctgtgct gggggccctg ggcagggccc 481 tgagcccctt ggaggagtgg cttcggctgc acacctactt ggccggggag gcccccactc 541 tggctgacct ggcggctgtc acagccttgc tgctgccttt ccgatacgtc ctagacccac 601 ctgcccgccg gatctggaat aatgtgactc gctggtttgt cacgtgtgtc cggcagccag 661 aattccgagc cgtgctagga gaagtggttc tatactcagg agccaggcct ctctctcatc 721 agccaggccc cgaggctcct gccctcccaa agacagctgc tcagctcaag aaagaggcaa 781 agaaacggga gaagctagag aaattccaac agaagcagaa gatccaacag cagcagccac 841 ctccagggga gaagaaacca aaaccagaga agagggagaa acgggatcct ggggtcatta 901 cctatgacct cccaacccca cccggggaaa agaaagatgt cagtggcccc atgcccgact 961 cctacagccc tcggtatgtg gaggctgcct ggtacccttg gtgggagcag cagggcttct 1021 tcaagccaga gtatgggcgt cctaatgtgt cagcagcaaa tccccgaggt gtcttcatga 1081 tgtgcatccc accccccaat gtgacaggct ccctgcacct gggccatgca ctcaccaacg 1141 ccatccagga ctccctgact cgatggcacc gcatgcgtgg ggagaccacc ctgtggaacc 1201 ctggctgtga ccatgcaggt attgccaccc aggtggtggt ggagaagaag ctatggcgtg 1261 agcagggact gagccggcac cagctgggcc gcgaggcctt tctacaggaa gtctggaagt 1321 ggaaggagga gaaaggtgac cggatttacc accagttgaa gaagcttggc agctccttgg 1381 actgggatcg agcctgtttt accatggacc ctaaactctc agcagctgtg acagaggcct 1441 ttgtccggct tcacgaggaa ggcatcatct atcgcagtac ccgccttgtt aactggtcct 1501 gcaccctcaa ctccgccatc tctgacattg aggtggataa gaaggagctg acaggtcgca 1561 ccctgctctc cgtgcctggc tacaaggaga aggtggagtt cggggtcctc gtgtcctttg 1621 cctataaggt ccaaggctca gatagcgacg aggaggtggt ggtggcaaca actcggatcg 1681 agacaatgct gggagatgtg gctgtagctg tgcaccccaa agataccaga taccagcacc 1741 tgaaggggaa gaacgtgatc cacccattcc tgtctcggag ccttcccatt gtcttcgatg 1801 aatttgtgga catggacttt ggcacaggtg ctgtgaagat cacccccgca catgaccaaa 1861 atgactatga agttgggcag cggcacgggc tggaggccat cagcatcatg gactcccggg 1921 gggccctcat caatgtgcct ccgcctttcc tgggcctgcc caggtttgag gccaggaaag 1981 cggtgctggt ggcgctgaag gagcggggac tgttccgtgg cattgaggac aaccccatgg 2041 tggtgccact ttgcaaccgg tcgaaggacg tggtagagcc tctgctgcgg ccgcagtggt 2101 acgttcgctg cggggagatg gcccaggctg ccagcgccgc tgtgactcgg ggtgacctcc 2161 gcatcctgcc tgaggcccat cagcgcacat ggcatgcctg gatggacaac atccgggagt 2221 ggtgcatttc caggcagctg tggtggggcc atcgcatccc agcctacttt gtcactgtca 2281 gtgacccagc ggtgccccct ggggaggacc ctgatgggcg gtactgggtg agtggacgca 2341 atgaggcgga ggcccgggag aaggcagcca aggagttcgg agtgtcccct gacaagatca 2401 gtctccagca agatgaggat gtattggata cctggttctc ctctggcctc ttccccttat 2461 ccattttggg ctggcccaac cagtcagaag acctgagtgt gttctacccc gggacactgc 2521 tggagaccgg tcatgacatc ctcttcttct gggtggcccg gatggtcatg ctgggcctga 2581 agctcacggg caggctgccc tttagagagg tctacctcca tgccatcgtg cgagatgctc 2641 acggccggaa gatgagcaag tctctaggca atgtcatcga tcccctggac gtcatctatg 2701 gaatctccct gcagggcctc cacaaccagc tgctgaacag caacctggat cccagcgagg 2761 tggagaaggc caaagaaggg cagaaagctg acttcccagc ggggattcct gaatgtggca 2821 ccgatgctct ccggtttgga ttatgtgcct acatgtccca gggtcgtgac atcaacctgg 2881 atgtgaaccg gatactgggt taccgccact tctgcaacaa gctctggaat gccaccaagt 2941 ttgcccttcg tggccttggg aagggttttg tgccctcacc cacctcccag cccggaggcc 3001 atgagagcct ggtggaccgc tggatccgca gccgcctgac agaggctgtg aggctcagca 3061 atcaaggctt ccaggcctac gacttcccgg ccgtcaccac tgcccagtac agcttctggc 3121 tctatgagct ctgtgatgtc tacttggagt gcctgaaacc tgtactgaat ggggtggacc 3181 aggtggcagc tgagtgtgcc cgccagaccc tgtacacttg cctggacgtt ggcctgcggc 3241 tgctctcacc cttcatgccc ttcgtgacgg aggagctgtt ccagaggctg ccccggagga 3301 tgccgcaagc tccccctagc ctctgtgtta ccccctaccc ggagccctca gagtgctcct 3361 ggaaggaccc cgaggcagaa gccgcccttg agctggcgct aagcatcacg cgagccgtgc 3421 gctccctgcg ggccgactac aacctcaccc ggatccggcc tgactgtttc ctggaagtgg 3481 cggatgaggc cacgggcgcc ctggcatcgg cggtgtcggg ctacgtgcag gccctggcca 3541 gcgcaggtgt ggtggctgtt ctggccctgg gggctcccgc cccccagggt tgcgctgtgg 3601 ctctggcttc tgatcgctgc tccatccacc tgcagcttca ggggctggtg gaccctgcac 3661 gggagctggg caagctgcaa gccaagcgag ttgaggccca gcggcaggcc cagcgtctgc 3721 gggaacgccg tgctgcctcg ggctatcctg tcaaggtgcc gctcgaagtc caggaggcag 3781 atgaagccaa gctccaacag acagaagcag agctcaggaa ggtggatgag gccatcgccc 3841 tattccagaa gatgctgtgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 3901 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 3961 agtcgacaat caacctctgg atta