Transcript: Mouse XM_017317395.1

PREDICTED: Mus musculus valyl-tRNA synthetase (Vars), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Vars (22321)
Length:
4006
CDS:
144..3935

Additional Resources:

NCBI RefSeq record:
XM_017317395.1
NBCI Gene record:
Vars (22321)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017317395.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000076226 GCAAAGAAACGGGAGAAACTA pLKO.1 855 CDS 100% 5.625 3.938 N Vars n/a
2 TRCN0000076223 CCCGACTGTTTCTTGGAAGTA pLKO.1 3534 CDS 100% 4.950 3.465 N Vars n/a
3 TRCN0000222638 GCATTCTTGGATGGACAACAT pLKO.1 2267 CDS 100% 4.950 3.465 N Vars n/a
4 TRCN0000222639 GCCTACTTTATCACCGTCCAT pLKO.1 2337 CDS 100% 2.640 1.848 N Vars n/a
5 TRCN0000076225 CCCATCGTCTTCGATGACTTT pLKO.1 1860 CDS 100% 0.495 0.347 N Vars n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017317395.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07126 pDONR223 100% 87.7% 93.3% None (many diffs) n/a
2 ccsbBroad304_07126 pLX_304 0% 87.7% 93.3% V5 (many diffs) n/a
3 TRCN0000478609 GGGGTTCGCCCGCCTTGATCATTC pLX_317 9.4% 87.7% 93.3% V5 (many diffs) n/a
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