Construct: ORF ccsbBroad304_07198
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010769.1_s304c1, BRDN0000403801
- Derived from:
- ccsbBroadEn_07198
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CUL5 (8065)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07198
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8065 | CUL5 | cullin 5 | NM_003478.6 | 99.9% | 100% | 225A>G |
| 2 | human | 8065 | CUL5 | cullin 5 | XM_017018363.2 | 94.1% | 91% | (many diffs) |
| 3 | human | 8065 | CUL5 | cullin 5 | XM_005271682.2 | 92.3% | 92.4% | 225A>G;234_235ins177 |
| 4 | human | 8065 | CUL5 | cullin 5 | XM_017018364.1 | 66.5% | 66.5% | 0_1ins783 |
| 5 | human | 8065 | CUL5 | cullin 5 | XM_017018365.1 | 66.5% | 66.5% | 0_1ins783 |
| 6 | human | 8065 | CUL5 | cullin 5 | XM_011543013.2 | 49.7% | 47.2% | (many diffs) |
| 7 | mouse | 75717 | Cul5 | cullin 5 | NM_027807.3 | 82.7% | 89.8% | (many diffs) |
| 8 | mouse | 75717 | Cul5 | cullin 5 | NM_001161618.1 | 79.8% | 86.6% | (many diffs) |
| 9 | mouse | 75717 | Cul5 | cullin 5 | XM_017313680.1 | 75.5% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2406
- ORF length:
- 2340
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gacgtctaat ctgttaaaga ataaaggttc tcttcagttt gaagacaaat 121 gggattttat gcgcccgatt gttttgaagc ttttacgcca ggaatctgtt acaaaacagc 181 agtggtttga tctgttttcg gatgtgcatg cagtctgtct ttgggatgat aaaggcccag 241 caaaaattca tcaggcttta aaagaagata ttcttgagtt tattaagcag gcacaggcac 301 gagtactgag ccatcaagat gatacggctt tgctaaaagc atatattgtt gaatggcgaa 361 agttctttac acaatgtgat attttaccaa aacctttttg tcaactagag attactttaa 421 tgggtaaaca gggcagcaat aaaaaatcaa atgtggaaga cagtattgtt cgaaagctta 481 tgcttgatac atggaatgag tcaatctttt caaacataaa aaacagactc caagatagtg 541 caatgaagct ggtacatgct gagagattgg gagaagcttt tgattctcag ctggttattg 601 gagtaagaga atcctatgtt aacctttgtt ctaatcctga ggataaactt caaatttata 661 gggacaattt tgagaaggca tacttggatt caacagagag attttataga acacaagcac 721 cctcgtattt acaacaaaat ggtgtacaga attatatgaa atatgcagat gctaaattaa 781 aagaagaaga aaaacgagca ctacgttatt tagaaacaag acgagaatgt aactccgttg 841 aagcactcat ggaatgctgt gtaaatgccc tggtgacatc atttaaagag actatcttag 901 ctgagtgcca aggcatgatc aagagaaatg aaactgaaaa attacattta atgttttcat 961 tgatggacaa agttcctaat ggtatagagc caatgttgaa agacttggag gaacatatca 1021 ttagtgctgg cctggcagat atggtagcag ctgctgaaac tattactact gactctgaga 1081 aatacgttga gcagttactt acactattta atagatttag taaactcgtc aaagaagctt 1141 ttcaagatga tccacgattt cttactgcaa gagataaggc gtataaagca gttgttaatg 1201 atgctaccat atttaaactt gaattacctt tgaagcagaa gggggtggga ttaaaaactc 1261 agcctgaatc aaaatgccct gagctgcttg ccaattactg tgacatgttg ctaagaaaaa 1321 caccattaag caaaaaacta acctctgaag agattgaagc aaagcttaaa gaagtgctct 1381 tggtacttaa gtatgtacag aacaaagatg tttttatgag gtatcataaa gctcatttga 1441 cacgacgtct tatattagac atctctgccg atagtgaaat tgaagaaaac atggtagagt 1501 ggctaagaga agttggtatg ccagcggatt atgtaaacaa gcttgctaga atgtttcagg 1561 acataaaagt atctgaagat ttgaaccaag cttttaagga aatgcacaaa aataataaat 1621 tggcattacc agctgattca gttaatataa aaattctgaa tgctggcgcc tggtcaagaa 1681 gttctgagaa agtctttgtc tcacttccta ctgaactgga ggacttgata ccggaagtag 1741 aagaattcta caaaaaaaat catagtggta gaaaattaca ttggcatcat ctcatgtcaa 1801 atggaattat aacatttaag aatgaagttg gtcaatatga tttggaggta accacgtttc 1861 agctcgctgt attgtttgca tggaaccaaa gacccagaga gaaaatcagc tttgaaaatc 1921 ttaagcttgc aactgaactc cctgatgctg aacttaggag gactttatgg tctttagtag 1981 ctttcccaaa actcaaacgg caagttttgt tgtatgaacc tcaagtcaac tcacccaaag 2041 actttacaga aggtaccctc ttctcagtga accaggagtt cagtttaata aaaaatgcaa 2101 aggttcagaa aaggggtaaa atcaacttga ttggacgttt gcagctcact acagaaagga 2161 tgagagaaga agagaatgaa ggaatagttc aactacgaat actaagaacc caggaagcta 2221 tcatacaaat aatgaaaatg agaaagaaaa ttagtaatgc tcagctgcag actgaattag 2281 tagaaatttt gaaaaacatg ttcttgccac aaaagaaaat gataaaagag caaatagagt 2341 ggctaataga gcacaaatac atcagaagag atgaatctga tatcaacact ttcatatata 2401 tggcataccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2461 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2521 acctctggat ta