Construct: ORF ccsbBroad304_07264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009861.1_s304c1, BRDN0000408124
- Derived from:
- ccsbBroadEn_07264
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- THOC5 (8563)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07264
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8563 | THOC5 | THO complex 5 | NM_001002877.2 | 99.9% | 99.7% | 1573G>A;1735G>A |
| 2 | human | 8563 | THOC5 | THO complex 5 | NM_001002878.1 | 99.9% | 99.7% | 1573G>A;1735G>A |
| 3 | human | 8563 | THOC5 | THO complex 5 | NM_001002879.1 | 99.9% | 99.7% | 1573G>A;1735G>A |
| 4 | human | 8563 | THOC5 | THO complex 5 | NM_003678.5 | 99.9% | 99.7% | 1573G>A;1735G>A |
| 5 | human | 8563 | THOC5 | THO complex 5 | XM_024452296.1 | 99.9% | 99.7% | 1573G>A;1735G>A |
| 6 | human | 8563 | THOC5 | THO complex 5 | XM_005261797.1 | 98.3% | 98.1% | 847_879del;1606G>A;1768G>A |
| 7 | human | 8563 | THOC5 | THO complex 5 | XM_005261798.1 | 98.3% | 98.1% | 847_879del;1606G>A;1768G>A |
| 8 | human | 8563 | THOC5 | THO complex 5 | XM_005261799.1 | 98.3% | 98.1% | 847_879del;1606G>A;1768G>A |
| 9 | human | 8563 | THOC5 | THO complex 5 | XM_006724347.1 | 98.3% | 98.1% | 847_879del;1606G>A;1768G>A |
| 10 | human | 8563 | THOC5 | THO complex 5 | XM_017029027.2 | 98.3% | 98.1% | 847_879del;1606G>A;1768G>A |
| 11 | human | 8563 | THOC5 | THO complex 5 | XR_001755346.1 | 83.1% | (many diffs) | |
| 12 | human | 8563 | THOC5 | THO complex 5 | XM_005261801.4 | 50.2% | 50% | 0_1ins1017;556G>A;718G>A |
| 13 | mouse | 107829 | Thoc5 | THO complex 5 | NM_172438.3 | 89.7% | 96% | (many diffs) |
| 14 | mouse | 107829 | Thoc5 | THO complex 5 | XM_006514448.2 | 88.2% | 94.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2115
- ORF length:
- 2049
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttaccatgtc atcagaatcg agcaaaaaac ggaagcccaa agtgatccga agcgatggag 121 ccccagctga aggaaagcgg aatcgatctg acaccgagca ggaaggtaaa tactacagtg 181 aggaggccga ggtggatctg cgggaccctg gcagagacta tgagttatac aagtacacct 241 gccaggagct acagaggctg atggctgaga tccaagacct gaagagcagg ggtggcaagg 301 atgtggcaat agaaatagaa gaacggagga tccagagctg tgtgcatttc atgactctaa 361 agaagcttaa ccgattagcc cacatcaggt tgaagaaagg aagagatcag acccacgagg 421 ctaagcagaa agtagatgcc tatcatctgc agctccagaa cctgttgtat gaggtgatgc 481 acctacagaa ggagatcacc aaatgtttgg agtttaagtc aaagcatgaa gaaattgatc 541 tggtcagttt agaggagttt tataaggagg ctccaccaga tatcagcaag gccgaagtca 601 ccatgggaga ccctcaccag caaacactgg cacgtctgga ctgggagctg gagcagcgga 661 aaaggctggc agagaagtac cgagagtgcc tatctaacaa ggagaagatt ctcaaggaga 721 ttgaggtgaa gaaggagtac ctgagcagcc tccagccccg cctcaacagc atcatgcagg 781 cttcccttcc ggtgcaggag tacctgttta tgccattcga ccaggctcac aagcagtatg 841 agacagccag acacctgccg cctcccctct atgtcctctt tgttcaggcc actgcgtatg 901 ggcaggcctg tgataagacg ttatctgtgg caatcgaagg cagtgtggat gaagccaagg 961 ctctgttcaa acctccagag gactcccaag atgacgagag tgactcagat gccgaggagg 1021 agcagactac gaagcgccgg agacccacac tgggggttca gttggacgac aaacgcaagg 1081 agatgctgaa gaggcaccca ctgtctgtca tgctcgacct gaagtgcaaa gatgacagtg 1141 tgcttcacct gactttctac tacctcatga acctcaacat catgacagta aaagccaaag 1201 tgacaactgc catggagctg atcaccccca tcagtgcagg tgacttgctg tctcctgact 1261 cagtcctgag ttgcttgtat cctggggatc atggaaagaa aactccgaat ccagccaatc 1321 agtatcagtt tgataaagtt ggcatcctga ctttgagcga ctatgtactt gagctaggtc 1381 acccctattt gtgggtgcag aagctgggtg gcctccactt ccccaaagag cagccccagc 1441 aaacagtgat tgctgaccac tcgctgagcg ccagccacat ggagaccacc atgaaacttc 1501 tgaagaccag ggtgcagtcc cgcctggccc tccacaaaca gtttgcatcc ctagaacatg 1561 gcattgtgcc agttaccagt gattgccagt acctcttccc tgccaaggtt gtctctcgcc 1621 tggtgaaatg ggtgacaatt gcccatgagg attacatgga gctgcacttc accaaagaca 1681 ttgtggatgc gggactggct ggggacacca atctctacta catggcgctc atcgaaaggg 1741 gcacagccaa actgcaggcc gctgtggtgt tgaaccctgg ctactcctcc atcccaccta 1801 ttttccagct ctgtttgaac tggaaagggg agaaaaccaa cagcaacgat gacaacattc 1861 gggccatgga gggcgaagtc aatgtgtgct acaaggagct gtgtggccct tggcccagcc 1921 accagctgtt gaccaaccag ctgcagcggc tgtgtgtgct gctggatgtt tacctggaga 1981 ccgagagcca tgacgacagt gtggaggggc ccaaggaatt tccccaggag aagatgtgtc 2041 tgcggctctt caggggtcct agcaggatga agccatttaa atacaaccat cctcagggat 2101 tcttcagcca tcgctgatct cctttcttgt acaaagtggt tggtaagcct atccctaacc 2161 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2221 cgacaatcaa cctctggatt a