Construct: ORF ccsbBroad304_07264

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009861.1_s304c1, BRDN0000408124
Derived from:
ccsbBroadEn_07264
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
THOC5 (8563)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07264
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8563 THOC5 THO complex 5 NM_001002877.2 99.9% 99.7% 1573G>A;1735G>A
2 human 8563 THOC5 THO complex 5 NM_001002878.1 99.9% 99.7% 1573G>A;1735G>A
3 human 8563 THOC5 THO complex 5 NM_001002879.1 99.9% 99.7% 1573G>A;1735G>A
4 human 8563 THOC5 THO complex 5 NM_003678.5 99.9% 99.7% 1573G>A;1735G>A
5 human 8563 THOC5 THO complex 5 XM_024452296.1 99.9% 99.7% 1573G>A;1735G>A
6 human 8563 THOC5 THO complex 5 XM_005261797.1 98.3% 98.1% 847_879del;1606G>A;1768G>A
7 human 8563 THOC5 THO complex 5 XM_005261798.1 98.3% 98.1% 847_879del;1606G>A;1768G>A
8 human 8563 THOC5 THO complex 5 XM_005261799.1 98.3% 98.1% 847_879del;1606G>A;1768G>A
9 human 8563 THOC5 THO complex 5 XM_006724347.1 98.3% 98.1% 847_879del;1606G>A;1768G>A
10 human 8563 THOC5 THO complex 5 XM_017029027.2 98.3% 98.1% 847_879del;1606G>A;1768G>A
11 human 8563 THOC5 THO complex 5 XR_001755346.1 83.1% (many diffs)
12 human 8563 THOC5 THO complex 5 XM_005261801.4 50.2% 50% 0_1ins1017;556G>A;718G>A
13 mouse 107829 Thoc5 THO complex 5 NM_172438.3 89.7% 96% (many diffs)
14 mouse 107829 Thoc5 THO complex 5 XM_006514448.2 88.2% 94.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2115
ORF length:
2049
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttaccatgtc atcagaatcg agcaaaaaac ggaagcccaa agtgatccga agcgatggag
121ccccagctga aggaaagcgg aatcgatctg acaccgagca ggaaggtaaa tactacagtg
181aggaggccga ggtggatctg cgggaccctg gcagagacta tgagttatac aagtacacct
241gccaggagct acagaggctg atggctgaga tccaagacct gaagagcagg ggtggcaagg
301atgtggcaat agaaatagaa gaacggagga tccagagctg tgtgcatttc atgactctaa
361agaagcttaa ccgattagcc cacatcaggt tgaagaaagg aagagatcag acccacgagg
421ctaagcagaa agtagatgcc tatcatctgc agctccagaa cctgttgtat gaggtgatgc
481acctacagaa ggagatcacc aaatgtttgg agtttaagtc aaagcatgaa gaaattgatc
541tggtcagttt agaggagttt tataaggagg ctccaccaga tatcagcaag gccgaagtca
601ccatgggaga ccctcaccag caaacactgg cacgtctgga ctgggagctg gagcagcgga
661aaaggctggc agagaagtac cgagagtgcc tatctaacaa ggagaagatt ctcaaggaga
721ttgaggtgaa gaaggagtac ctgagcagcc tccagccccg cctcaacagc atcatgcagg
781cttcccttcc ggtgcaggag tacctgttta tgccattcga ccaggctcac aagcagtatg
841agacagccag acacctgccg cctcccctct atgtcctctt tgttcaggcc actgcgtatg
901ggcaggcctg tgataagacg ttatctgtgg caatcgaagg cagtgtggat gaagccaagg
961ctctgttcaa acctccagag gactcccaag atgacgagag tgactcagat gccgaggagg
1021agcagactac gaagcgccgg agacccacac tgggggttca gttggacgac aaacgcaagg
1081agatgctgaa gaggcaccca ctgtctgtca tgctcgacct gaagtgcaaa gatgacagtg
1141tgcttcacct gactttctac tacctcatga acctcaacat catgacagta aaagccaaag
1201tgacaactgc catggagctg atcaccccca tcagtgcagg tgacttgctg tctcctgact
1261cagtcctgag ttgcttgtat cctggggatc atggaaagaa aactccgaat ccagccaatc
1321agtatcagtt tgataaagtt ggcatcctga ctttgagcga ctatgtactt gagctaggtc
1381acccctattt gtgggtgcag aagctgggtg gcctccactt ccccaaagag cagccccagc
1441aaacagtgat tgctgaccac tcgctgagcg ccagccacat ggagaccacc atgaaacttc
1501tgaagaccag ggtgcagtcc cgcctggccc tccacaaaca gtttgcatcc ctagaacatg
1561gcattgtgcc agttaccagt gattgccagt acctcttccc tgccaaggtt gtctctcgcc
1621tggtgaaatg ggtgacaatt gcccatgagg attacatgga gctgcacttc accaaagaca
1681ttgtggatgc gggactggct ggggacacca atctctacta catggcgctc atcgaaaggg
1741gcacagccaa actgcaggcc gctgtggtgt tgaaccctgg ctactcctcc atcccaccta
1801ttttccagct ctgtttgaac tggaaagggg agaaaaccaa cagcaacgat gacaacattc
1861gggccatgga gggcgaagtc aatgtgtgct acaaggagct gtgtggccct tggcccagcc
1921accagctgtt gaccaaccag ctgcagcggc tgtgtgtgct gctggatgtt tacctggaga
1981ccgagagcca tgacgacagt gtggaggggc ccaaggaatt tccccaggag aagatgtgtc
2041tgcggctctt caggggtcct agcaggatga agccatttaa atacaaccat cctcagggat
2101tcttcagcca tcgctgatct cctttcttgt acaaagtggt tggtaagcct atccctaacc
2161ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2221cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)