Construct: ORF ccsbBroad304_07320

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008997.1_s304c1, BRDN0000399030
Derived from:
ccsbBroadEn_07320
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SQSTM1 (8878)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07320
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8878 SQSTM1 sequestosome 1 NM_003900.5 99.7% 99.7% 876C>T;905G>A;936G>A
2 human 8878 SQSTM1 sequestosome 1 NM_001142298.2 80.6% 80.6% (many diffs)
3 human 8878 SQSTM1 sequestosome 1 NM_001142299.2 80.6% 80.6% (many diffs)
4 mouse 18412 Sqstm1 sequestosome 1 NM_011018.3 86.4% 90.4% (many diffs)
5 mouse 18412 Sqstm1 sequestosome 1 NM_001290769.1 78.4% 81.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1386
ORF length:
1320
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gtcgctcacc gtgaaggcct accttctggg caaggaggac gcggcgcgcg
121agattcgccg cttcagcttc tgctgcagcc ccgagcctga ggcggaagcc gaggctgcgg
181cgggtccggg accctgcgag cggctgctga gccgggtggc cgccctgttc cccgcgctgc
241ggcctggcgg cttccaggcg cactaccgcg atgaggacgg ggacttggtt gccttttcca
301gtgacgagga attgacaatg gccatgtcct acgtgaagga tgacatcttc cgaatctaca
361ttaaagagaa aaaagagtgc cggcgggacc accgcccacc gtgtgctcag gaggcgcccc
421gcaacatggt gcaccccaat gtgatctgcg atggctgcaa tgggcctgtg gtaggaaccc
481gctacaagtg cagcgtctgc ccagactacg acttgtgtag cgtctgcgag ggaaagggct
541tgcaccgggg gcacaccaag ctcgcattcc ccagcccctt cgggcacctg tctgagggct
601tctcgcacag ccgctggctc cggaaggtga aacacggaca cttcgggtgg ccaggatggg
661aaatgggtcc accaggaaac tggagcccac gtcctcctcg tgcaggggag gcccgccctg
721gccccacggc agaatcagct tctggtccat cggaggatcc gagtgtgaat ttcctgaaga
781acgttgggga gagtgtggca gctgccctta gccctctggg cattgaagtt gatatcgatg
841tggagcacgg agggaaaaga agccgcctga cccccgtctc tccagagagt tccagcacag
901aggagaagag cagctcacag ccaagcagct gctgctctga tcccagcaag ccgggtggga
961atgttgagga cgccacgcag tctctggcgg agcagatgag aaagatcgcc ttggagtccg
1021aggggcgccc tgaggaacag atggagtcgg ataactgttc aggaggagat gatgactgga
1081cccatctgtc ttcaaaagaa gtggacccgt ctacaggtga actccagtcc ctacagatgc
1141cagaatccga agggccaagc tctctggacc cctcccagga gggacccaca gggctgaagg
1201aagctgcctt gtacccacat ctcccgccag aggctgaccc gcggctgatt gagtccctct
1261cccagatgct gtccatgggc ttctctgatg aaggcggctg gctcaccagg ctcctgcaga
1321ccaagaacta tgacatcgga gcggctctgg acaccatcca gtattcaaag catcccccgc
1381cgttgtgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1441gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1501acctctggat ta

Download FASTA (ORF) (Full)