Construct: ORF ccsbBroad304_07334

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017911.1_s304c1, BRDN0000400634
Derived from:
ccsbBroadEn_07334
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WASL (8976)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07334
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8976 WASL WASP like actin nucleation ... NM_003941.4 99.9% 100% 1140A>C
2 mouse 73178 Wasl Wiskott-Aldrich syndrome-li... NM_028459.2 90.1% 95.4% (many diffs)
3 mouse 73178 Wasl Wiskott-Aldrich syndrome-li... NM_001167745.1 31.9% 29.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1581
ORF length:
1515
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag ctccgtccag cagcagccgc cgccgccgcg gagggtcacc aacgtggggt
121ccctgttgct caccccgcag gagaacgagt ccctcttcac tttcctcggc aagaaatgtg
181tgactatgtc ttcagcagtg gtgcagttat atgcagcaga tcggaactgt atgtggtcaa
241agaagtgcag tggtgttgct tgtcttgtta aggacaatcc acagagatct tattttttaa
301gaatatttga cattaaggat gggaaactat tgtgggaaca agagctatac aataactttg
361tatataatag tcctagagga tattttcata cctttgctgg agatacttgt caagttgctc
421ttaattttgc caatgaagaa gaagcaaaaa aatttcgaaa agcagttaca gaccttttgg
481gccgtcgaca aaggaaatct gagaaaagac gagatccccc aaatggtcct aatctaccca
541tggctacagt tgatataaaa aatccagaaa tcacaacaaa tagattttat ggtccacaag
601tcaacaacat ctcccatacc aaagaaaaga agaagggaaa agctaaaaag aagagattaa
661ccaaggcaga tataggaaca ccaagcaatt tccagcacat tggacatgtt ggttgggatc
721caaatacagg ctttgatctg aataatttgg atccagaatt gaagaatctt ttcgatatgt
781gtggaatctc agaggcacaa cttaaagaca gagaaacatc aaaagttata tatgacttta
841ttgaaaaaac aggaggtgtt gaagctgtta aaaatgaact gcggaggcaa gcaccaccac
901ctccaccacc atcaagggga gggccacctc ctcctcctcc ccctccacac aactcaggtc
961ctcctcctcc tcctgctagg ggaagaggcg ctcctccccc accaccttca agagctccca
1021cagctgcacc tccaccaccg cctccttcca ggccaagtgt agcagtccct ccaccaccgc
1081caaataggat gtaccctcct ccacctccag cccttccctc ctcagcacct tcagggcctc
1141caccaccacc tccatctgtg ttgggggtag ggccagtggc accaccccca ccgcctccac
1201ctccccctcc tcctgggcca ccgcccccgc ctggcctgcc ttctgatggg gaccatcagg
1261ttccaactac tgcaggaaac aaagcagctc ttttagatca aattagagag ggtgctcagc
1321taaaaaaagt ggagcagaac agtcggccag tgtcctgctc tggacgagat gcactgttag
1381accagatacg acagggtatc caactaaaat ctgtggctga tggccaagag tctacaccac
1441caacacctgc acccacttca ggaattgtgg gtgcattaat ggaagtgatg cagaaaagga
1501gcaaagccat tcattcttca gatgaagatg aagatgaaga tgatgaagaa gattttgagg
1561atgatgatga gtgggaagac tgcccaactt tcttgtacaa agtggttggt aagcctatcc
1621ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1681ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)