Transcript: Mouse NM_028459.2

Mus musculus Wiskott-Aldrich syndrome-like (human) (Wasl), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Wasl (73178)
Length:
4348
CDS:
353..1858

Additional Resources:

NCBI RefSeq record:
NM_028459.2
NBCI Gene record:
Wasl (73178)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_028459.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000099640 GCCTCTTTACAGTACACTAAT pLKO.1 3030 3UTR 100% 13.200 18.480 N Wasl n/a
2 TRCN0000123060 CGGCAAGAAATGTGTGACTAT pLKO.1 445 CDS 100% 4.950 3.960 N WASL n/a
3 TRCN0000289046 CGGCAAGAAATGTGTGACTAT pLKO_005 445 CDS 100% 4.950 3.960 N WASL n/a
4 TRCN0000099643 AGATTAACCAAGGCAGATATT pLKO.1 932 CDS 100% 13.200 9.240 N Wasl n/a
5 TRCN0000123062 CAGGAAACAAAGCAGCTCTTT pLKO.1 1548 CDS 100% 4.950 3.465 N WASL n/a
6 TRCN0000288996 CAGGAAACAAAGCAGCTCTTT pLKO_005 1548 CDS 100% 4.950 3.465 N WASL n/a
7 TRCN0000099641 GCTGGAGATACTTGTCAAGTA pLKO.1 674 CDS 100% 4.950 3.465 N Wasl n/a
8 TRCN0000099644 TCAGGAAACAAAGCAGCTCTT pLKO.1 1547 CDS 100% 4.050 2.835 N Wasl n/a
9 TRCN0000099642 GCTTGTCTGGTTAAGGACAAT pLKO.1 536 CDS 100% 0.495 0.347 N Wasl n/a
10 TRCN0000123063 GCTGGAGATACTTGTCAAGTT pLKO.1 674 CDS 100% 4.950 3.465 N WASL n/a
11 TRCN0000307001 GCTGGAGATACTTGTCAAGTT pLKO_005 674 CDS 100% 4.950 3.465 N WASL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_028459.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07334 pDONR223 100% 90.1% 95.4% None (many diffs) n/a
2 ccsbBroad304_07334 pLX_304 0% 90.1% 95.4% V5 (many diffs) n/a
3 TRCN0000467204 CCGGTGTTAACATGATCCTAAAGC pLX_317 25.4% 90.1% 95.4% V5 (many diffs) n/a
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