Construct: ORF ccsbBroad304_07351
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017341.1_s304c1, BRDN0000405044
- Derived from:
- ccsbBroadEn_07351
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC7A7 (9056)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07351
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9056 | SLC7A7 | solute carrier family 7 mem... | NM_001126105.3 | 99.9% | 100% | 1527A>G |
| 2 | human | 9056 | SLC7A7 | solute carrier family 7 mem... | NM_001126106.4 | 99.9% | 100% | 1527A>G |
| 3 | human | 9056 | SLC7A7 | solute carrier family 7 mem... | NM_003982.4 | 99.9% | 100% | 1527A>G |
| 4 | human | 9056 | SLC7A7 | solute carrier family 7 mem... | XM_006720302.2 | 99.9% | 100% | 1527A>G |
| 5 | human | 9056 | SLC7A7 | solute carrier family 7 mem... | XM_011537298.3 | 99.9% | 100% | 1527A>G |
| 6 | human | 9056 | SLC7A7 | solute carrier family 7 mem... | XM_011537299.1 | 99.9% | 100% | 1527A>G |
| 7 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | NM_001253679.1 | 87.5% | 90% | (many diffs) |
| 8 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | NM_001253680.1 | 87.5% | 90% | (many diffs) |
| 9 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | NM_011405.4 | 87.5% | 90% | (many diffs) |
| 10 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | XM_006518748.2 | 87.5% | 90% | (many diffs) |
| 11 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | XM_006518751.2 | 87.5% | 90% | (many diffs) |
| 12 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | XM_006518752.1 | 87.5% | 90% | (many diffs) |
| 13 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | XM_006518753.1 | 87.5% | 90% | (many diffs) |
| 14 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | XM_006518754.3 | 87.5% | 90% | (many diffs) |
| 15 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | XM_006518755.3 | 87.5% | 90% | (many diffs) |
| 16 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | XM_006518746.2 | 83.8% | 86.1% | (many diffs) |
| 17 | mouse | 20540 | Slc7a7 | solute carrier family 7 (ca... | XM_011245000.2 | 49.1% | 50% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1599
- ORF length:
- 1533
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tcaccatggt tgacagcact gagtatgaag tggcctccca gcctgaggtg gaaacctccc 121 ctttgggtga tggggccagc ccagggccgg agcaggtgaa gctgaagaag gagatctcac 181 tgcttaacgg cgtgtgcctg attgtgggga acatgatcgg ctcgggcatc tttgtttccc 241 ccaagggtgt gctcatatac agtgcctcct ttggtctctc tctggtcatc tgggctgtcg 301 ggggcctctt ctccgtcttt ggggcccttt gttatgcgga actgggcacc accattaaga 361 aatctggggc cagctatgcc tatatcctgg aggcctttgg aggattcctt gctttcatca 421 gactctggac ctccctgctc atcattgagc ccaccagcca ggccatcatt gccatcacct 481 ttgccaacta catggtacag cctctcttcc cgagctgctt cgccccttat gctgccagcc 541 gcctgctggc tgctgcctgc atttgtctct taaccttcat taactgtgcc tatgtcaaat 601 ggggaaccct ggtacaagat attttcacct atgctaaagt attggcactg atcgcggtca 661 tcgttgcagg cattgttaga cttggccagg gagcctctac tcattttgag aattcctttg 721 agggttcatc atttgcagtg ggtgacattg ccctggcact gtactcagct ctgttctcct 781 actcaggctg ggacaccctc aactatgtca ctgaagagat caagaatcct gagaggaacc 841 tgcccctctc cattggcatc tccatgccca ttgtcaccat catctatatc ttgaccaatg 901 tggcctatta tactgtgcta gacatgagag acatcttggc cagtgatgct gttgctgtga 961 cttttgcaga tcagatattt ggaatattta actggataat tccactgtca gttgcattat 1021 cctgttttgg tggcctcaat gcctccattg tggctgcttc taggcttttc tttgtgggct 1081 caagagaagg ccatctccct gatgccatct gcatgatcca tgttgagcgg ttcacaccag 1141 tgccttctct gctcttcaat ggtatcatgg cattgatcta cttgtgcgtg gaagacatct 1201 tccagctcat taactactac agcttcagct actggttctt tgtggggctt tctattgtgg 1261 gtcagcttta tctgcgctgg aaggagcctg atcgacctcg tcccctcaag ctcagcgttt 1321 tcttcccgat tgtcttctgc ctctgcacca tcttcctggt ggctgttcca ctttacagtg 1381 atactatcaa ctccctcatc ggcattgcca ttgccctctc aggcctgccc ttttacttcc 1441 tcatcatcag agtgccagaa cataagcgac cgctttacct ccgaaggatc gtggggtctg 1501 ccacaaggta cctccaggtc ctgtgtatgt cagttgctgc agaaatggat ttggaagatg 1561 gaggagagat gcccaagcaa cgggatccca agtctaacta aacacctttc ttgtacaaag 1621 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1681 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta