Construct: ORF ccsbBroad304_07402
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011035.1_s304c1, BRDN0000400587
- Derived from:
- ccsbBroadEn_07402
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB28 (9364)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07402
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9364 | RAB28 | RAB28, member RAS oncogene ... | NM_001017979.3 | 99.8% | 100% | 24C>T |
| 2 | human | 9364 | RAB28 | RAB28, member RAS oncogene ... | NM_004249.4 | 92.4% | 91.8% | (many diffs) |
| 3 | human | 9364 | RAB28 | RAB28, member RAS oncogene ... | NM_001159601.1 | 87.4% | 87.7% | (many diffs) |
| 4 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_027295.3 | 91.8% | 95.4% | (many diffs) |
| 5 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_001310589.1 | 85.5% | 87.1% | (many diffs) |
| 6 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | XM_017320585.1 | 85.5% | 87.1% | (many diffs) |
| 7 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | XM_006503651.3 | 84.8% | 83.7% | (many diffs) |
| 8 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_001310590.1 | 80% | 82.8% | (many diffs) |
| 9 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_001310592.1 | 59.8% | 61.9% | (many diffs) |
| 10 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NR_132325.1 | 31.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggactctgag gaggagagtc aggaccggca actgaaaatc gtcgtgctgg 121 gggacggcgc ctccgggaag acctccttaa ctacgtgttt tgctcaagaa acttttggga 181 aacagtacaa acaaactata ggactggatt tctttttgag aaggataaca ttgccaggaa 241 acttgaatgt tacccttcaa atttgggata taggagggca gacaatagga ggcaaaatgt 301 tggataaata tatctatgga gcacagggag tcctcttggt atatgatatt acaaattatc 361 aaagctttga gaatttagaa gattggtata ctgtggtgaa gaaagtgagc gaggagtcag 421 aaactcagcc actggttgcc ttggtaggca ataaaattga tttggagcat atgcgaacaa 481 taaaacctga aaaacactta cggttttgcc aggaaaatgg ttttagtagc cactttgtct 541 cagccaagac aggagactct gtcttcctgt gctttcagaa agttgctgct gaaatccttg 601 ggatcaaatt aaacaaagca gaaatagaac agtcacagag ggtggtgaag gcagatattg 661 taaactacaa ccaggaacct atgtcaagga ctgttaaccc tcctagaagc tctatgtgtg 721 cagttcagtg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 781 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 841 tcaacctctg gatta