Construct: ORF ccsbBroad304_07409

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010316.1_s304c1, BRDN0000462357
Derived from:
ccsbBroadEn_07409
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HS6ST1 (9394)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07409
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9394 HS6ST1 heparan sulfate 6-O-sulfotr... NM_004807.3 99.9% 100% 177A>G
2 mouse 50785 Hs6st1 heparan sulfate 6-O-sulfotr... NM_015818.2 91% 97.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1302
ORF length:
1233
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcggcggcgg cgcgccggcg gcaggaccat ggttgagcgc gccagcaagt
121tcgtgctggt ggtggcgggc tcggtgtgct tcatgctcat cttgtaccag tacgcgggcc
181caggactgag cctgggcgcg cccggcggcc gcgcgccgcc cgacgacctg gacctgttcc
241ccacgcccga cccccactac gagaagaagt actacttccc ggtccgcgag ctggagcgct
301cgctgcgctt cgacatgaag ggcgacgacg tgatcgtctt cctgcacatc cagaagacgg
361gcggcaccac cttcggccgc cacctcgtgc agaacgtacg cctcgaggtg ccgtgcgact
421gccggcccgg ccagaagaag tgcacctgct accggcccaa ccgccgcgag acttggctct
481tctcccgctt ctccaccggc tggagctgcg ggctgcacgc cgactggacc gagctcacca
541actgcgtgcc cggcgtgctg gaccgccgcg actccgccgc gctgcgcacg cccaggaagt
601tctactacat caccctgcta cgagaccccg tgtcccgcta cctgagcgag tggcggcatg
661tgcagagggg tgccacgtgg aagacgtcgt tgcatatgtg tgatgggcgc acgcccacgc
721ctgaggagct gccgccctgc tacgagggca cggactggtc gggctgcacg ctacaggagt
781tcatggactg cccgtacaac ctggccaaca accgccaggt gcgcatgctg gccgacctga
841gcctggtggg ctgctacaac ctgtccttca tccccgaggg caagcgggcc cagctgctgc
901tcgagagcgc caagaagaac ctgcggggca tggccttctt cggcctgacc gagttccagc
961gcaagacgca gtacctgttc gagcggacgt tcaacctcaa gttcatccgg cccttcatgc
1021agtacaatag cacgcgggcg ggcggcgtgg aggtggatga agacaccatc cggcgcatcg
1081aggagctcaa cgacctggac atgcagctgt acgactacgc caaggacctc ttccagcagc
1141gctaccagta caagcggcag ctggagcgca gggagcagcg cctgaggagc cgcgaggagc
1201gtctgctgca ccgggccaag gaggcactgc cgcgggagga tgccgacgag ccgggccgcg
1261tgcccaccga ggactacatg agccacatca ttgagaagtg gttgccaact ttcttgtaca
1321aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1381ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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