Transcript: Human NM_004807.3

Homo sapiens heparan sulfate 6-O-sulfotransferase 1 (HS6ST1), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
HS6ST1 (9394)
Length:
4223
CDS:
306..1541

Additional Resources:

NCBI RefSeq record:
NM_004807.3
NBCI Gene record:
HS6ST1 (9394)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004807.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000036006 CGAGGACTACATGAGCCACAT pLKO.1 1505 CDS 100% 4.050 5.670 N HS6ST1 n/a
2 TRCN0000440869 GTGTGTCCCTCACCAACTATG pLKO_005 1907 3UTR 100% 10.800 8.640 N HS6ST1 n/a
3 TRCN0000427074 GTGGAAGACGTCGTTGCATAT pLKO_005 914 CDS 100% 10.800 7.560 N HS6ST1 n/a
4 TRCN0000036004 CGGCCCTTCATGCAGTACAAT pLKO.1 1245 CDS 100% 5.625 3.938 N HS6ST1 n/a
5 TRCN0000036005 CCACTACGAGAAGAAGTACTA pLKO.1 491 CDS 100% 4.950 3.465 N HS6ST1 n/a
6 TRCN0000036007 CTGCTACAACCTGTCCTTCAT pLKO.1 1088 CDS 100% 4.950 3.465 N HS6ST1 n/a
7 TRCN0000415657 GACGTTCAACCTCAAGTTCAT pLKO_005 1223 CDS 100% 4.950 3.465 N HS6ST1 n/a
8 TRCN0000034564 GCCCAGGAAGTTCTACTACAT pLKO.1 827 CDS 100% 4.950 3.465 N HS6ST1P1 n/a
9 TRCN0000034567 CATGCTCATCTTGTACCAGTA pLKO.1 389 CDS 100% 4.050 2.835 N HS6ST1P1 n/a
10 TRCN0000036008 CGTGATCGTCTTCCTGCACAT pLKO.1 566 CDS 100% 4.050 2.835 N HS6ST1 n/a
11 TRCN0000034565 CCACATCATTGAGAAGTGGTA pLKO.1 1520 CDS 100% 2.640 1.848 N HS6ST1P1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004807.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07409 pDONR223 100% 99.9% 100% None 177A>G n/a
2 ccsbBroad304_07409 pLX_304 0% 99.9% 100% V5 177A>G n/a
3 TRCN0000481249 CCCATCACGTCTCGATTCCGTTTG pLX_317 30% 99.9% 100% V5 177A>G n/a
4 ccsbBroadEn_11364 pDONR223 100% 97.4% 97.5% None 1_30del;177A>G n/a
5 ccsbBroad304_11364 pLX_304 0% 97.4% 97.5% V5 1_30del;177A>G n/a
6 TRCN0000471834 GTATGAATAACCAATTCTACAACC pLX_317 31.8% 97.4% 97.5% V5 1_30del;177A>G n/a
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