Construct: ORF ccsbBroad304_07426
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016383.1_s304c1, BRDN0000410076
- Derived from:
- ccsbBroadEn_07426
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHST10 (9486)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07426
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9486 | CHST10 | carbohydrate sulfotransfera... | NM_004854.5 | 99.9% | 100% | 696C>G |
| 2 | human | 9486 | CHST10 | carbohydrate sulfotransfera... | XM_011512207.2 | 99.9% | 100% | 696C>G |
| 3 | human | 9486 | CHST10 | carbohydrate sulfotransfera... | XM_011512208.1 | 99.9% | 100% | 696C>G |
| 4 | human | 9486 | CHST10 | carbohydrate sulfotransfera... | XM_011512210.1 | 99.9% | 100% | 696C>G |
| 5 | human | 9486 | CHST10 | carbohydrate sulfotransfera... | XM_011512211.1 | 99.9% | 100% | 696C>G |
| 6 | human | 9486 | CHST10 | carbohydrate sulfotransfera... | XM_017005380.2 | 99.9% | 100% | 696C>G |
| 7 | human | 9486 | CHST10 | carbohydrate sulfotransfera... | XM_017005381.2 | 99.9% | 100% | 696C>G |
| 8 | human | 9486 | CHST10 | carbohydrate sulfotransfera... | XM_017005382.2 | 99.9% | 100% | 696C>G |
| 9 | human | 9486 | CHST10 | carbohydrate sulfotransfera... | XM_017005383.2 | 99.9% | 100% | 696C>G |
| 10 | human | 9486 | CHST10 | carbohydrate sulfotransfera... | XM_024453248.1 | 99.9% | 100% | 696C>G |
| 11 | mouse | 98388 | Chst10 | carbohydrate sulfotransfera... | XM_006496365.3 | 86.6% | 91% | (many diffs) |
| 12 | mouse | 98388 | Chst10 | carbohydrate sulfotransfera... | NM_145142.2 | 82.4% | 86.6% | (many diffs) |
| 13 | mouse | 98388 | Chst10 | carbohydrate sulfotransfera... | XM_006496364.3 | 82.4% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1134
- ORF length:
- 1068
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca ccaccagtgg cttctgctgg ccgcatgctt ttgggtgatt ttcatgttca 121 tggtggctag caagttcatc acgttgacct ttaaagaccc agatgtgtac agtgccaaac 181 aggagtttct gttcctgaca accatgccgg aagtgaggaa gttgccagaa gagaagcaca 241 ttcctgagga actgaagcca actgggaagg agcttccaga cagccagctc gttcagcccc 301 tggtctacat ggagcgcctg gaactcatca gaaacgtctg cagggatgat gccctgaaga 361 atctctcgca cactcctgtc tccaagtttg tcctggaccg aatatttgtc tgtgacaagc 421 acaagattct tttctgccag actcccaaag tgggcaacac ccagtggaag aaagtgctga 481 ttgttctaaa tggagcattt tcttccattg aggagatccc cgaaaacgtg gtgcacgacc 541 acgagaagaa cggccttcct cggctctctt ccttcagtga tgcagaaatt cagaagcgat 601 tgaaaacata cttcaagttt tttattgtaa gagatccctt cgaaagactt atttctgcat 661 ttaaggataa atttgttcac aatccccggt ttgagccttg gtacaggcat gagattgctc 721 ctggcatcat cagaaaatac aggaggaacc ggacagagac gcgggggatc cagtttgaag 781 atttcgtgcg ctacctcggc gatccgaacc acagatggct agaccttcag tttggggacc 841 acatcattca ctgggtgacg tatgtagagc tctgtgctcc ctgtgagata atgtacagtg 901 tgattggaca ccacgagacc ctggaggacg atgccccata catcttaaaa gaggctggca 961 ttgaccacct ggtgtcatac ccgactatcc ctccgggcat taccgtgtat aacagaacca 1021 aggtggagca ctatttcctg ggcatcagca aacgagacat ccgacgcctg tatgcccgtt 1081 tcgaagggga ctttaagctc tttgggtacc agaaaccaga ctttttgcta aactacccaa 1141 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1201 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta