Construct: ORF ccsbBroad304_07472
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010699.1_s304c1, BRDN0000400662
- Derived from:
- ccsbBroadEn_07472
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM131B (9715)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07472
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9715 | FAM131B | family with sequence simila... | NM_001031690.3 | 99.7% | 99.1% | 938A>G;956G>A;1003G>A |
| 2 | human | 9715 | FAM131B | family with sequence simila... | NM_001371248.1 | 96.3% | 95.8% | (many diffs) |
| 3 | human | 9715 | FAM131B | family with sequence simila... | NM_001371250.1 | 91.9% | 91.3% | (many diffs) |
| 4 | human | 9715 | FAM131B | family with sequence simila... | NM_014690.5 | 91.9% | 91.3% | (many diffs) |
| 5 | human | 9715 | FAM131B | family with sequence simila... | NM_001371249.1 | 88.6% | 88% | (many diffs) |
| 6 | human | 9715 | FAM131B | family with sequence simila... | NM_001371251.1 | 83% | 82.5% | (many diffs) |
| 7 | human | 9715 | FAM131B | family with sequence simila... | NM_001371252.1 | 83% | 82.5% | (many diffs) |
| 8 | human | 9715 | FAM131B | family with sequence simila... | NM_001371253.1 | 83% | 82.5% | (many diffs) |
| 9 | human | 9715 | FAM131B | family with sequence simila... | NM_001278297.2 | 73.6% | 73% | (many diffs) |
| 10 | mouse | 76156 | Fam131b | family with sequence simila... | NM_029528.5 | 90% | 95% | (many diffs) |
| 11 | mouse | 76156 | Fam131b | family with sequence simila... | XM_006506730.3 | 88.5% | 91.7% | (many diffs) |
| 12 | mouse | 76156 | Fam131b | family with sequence simila... | NM_001113327.2 | 86.9% | 91.6% | (many diffs) |
| 13 | mouse | 76156 | Fam131b | family with sequence simila... | NM_001286584.1 | 83% | 87.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1146
- ORF length:
- 1080
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcatcggc tcccggactg tggggaatga ggtgattgca gtggattgga 121 agggcctgaa ggatgtcgat caaatcaaca tggacagcac cagctcactg cacgggagca 181 gcctccatcg gccatcgact gagcaaactc gaactgattt ctcctgggac ggcatcaacc 241 tctccatgga ggacaccact tccattcttc cgaagcttaa gcgaaactct aacgcctatg 301 gcattggggc cctggccaag tcatcattct cagggatctc acggagcatg aaggaccatg 361 tgacaaagcc cacagccatg gggcaaggcc gggtggccca catgattgag tggcagggct 421 gggggaagac accagctgtt cagccacaac acagccatga gtccgtgcgc agggatacgg 481 atgcctactc cgacctcagc gatggcgaga aggaggcacg ttttctagca ggcgtcatgg 541 agcagtttgc tatctctgag gccacactca tggcctggtc ttccatggat ggtgaggaca 601 tgagtgtgaa ctccacccag gagccattgg gctgcaacta cagtgacaac taccaggaac 661 tgatggacag tcaggatgcc ctggctcaag cacccatgga tggatggcct cactcttacg 721 tgtcccaggg tatgtactgt ctggggtcgt cagatgcctg ggaagccagc gatcagtccc 781 tcattgcctc tccggccaca ggatcctatc ttgggcctgc atttgatgac tcacaaccca 841 gcttgcatga aatgggacct tcccaaccag cttcaggata ctctgctctg gagcctccac 901 ctttgctggg gggagacact gactgggctc cgggggtagg cgcagtggac ctggcaaggg 961 gccctgctga ggaggagaag aggccattgg cacctgagga gggagaggat gcgggatgcc 1021 aggacctgga gtcactttcc ccacgagaag accctgagat gtctaccact ctcagccgga 1081 aggtgtctga cgtcacatcc tcaggtgtgc agtcctttga tgaggaggaa ggcgaggcca 1141 acaactaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1201 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1261 acctctggat ta