Construct: ORF ccsbBroad304_07490
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004311.1_s304c1, BRDN0000400665
- Derived from:
- ccsbBroadEn_07490
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCRN1 (9805)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07490
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9805 | SCRN1 | secernin 1 | NM_001145513.1 | 99.9% | 99.7% | 893C>A |
| 2 | human | 9805 | SCRN1 | secernin 1 | NM_014766.5 | 99.9% | 99.7% | 893C>A |
| 3 | human | 9805 | SCRN1 | secernin 1 | XM_024447007.1 | 99.9% | 99.7% | 893C>A |
| 4 | human | 9805 | SCRN1 | secernin 1 | NM_001145514.1 | 95.3% | 95.1% | 1_60del;953C>A |
| 5 | human | 9805 | SCRN1 | secernin 1 | NM_001145515.2 | 83.4% | 83.3% | 0_1ins204;689C>A |
| 6 | mouse | 69938 | Scrn1 | secernin 1 | NM_027268.2 | 86.5% | 87.6% | (many diffs) |
| 7 | mouse | 69938 | Scrn1 | secernin 1 | XM_006506572.2 | 86.5% | 87.6% | (many diffs) |
| 8 | mouse | 69938 | Scrn1 | secernin 1 | XM_011241459.2 | 86.5% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1308
- ORF length:
- 1242
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcagctcct ccaagttact gttttgttgc cttccctcca cgtgctaagg 121 atggtctggt ggtatttggg aaaaattcag cccggcccag agatgaagtg caagaggttg 181 tgtatttctc ggctgctgat cacgaaccgg agagcaaggt tgagtgcact tacatttcaa 241 tcgaccaagt tccaaggacc tatgccataa tgataagcag acccgcctgg ctctggggag 301 cagaaatggg agccaatgaa catggagtgt gcatagccaa tgaagccatc aacaccagag 361 agccagctgc cgagatagaa gccttgctgg ggatggatct ggtcaggctt ggtttagaaa 421 gaggggaaac agctaaagaa gccttagatg tcattgtctc cttgttggaa gaacatggac 481 aaggtgggaa ttactttgaa gatgcaaact cctgccacag cttccaaagt gcatatctga 541 ttgtggatcg tgatgaagcc tgggtgctcg agaccatagg gaagtactgg gctgccgaga 601 aagtcacaga gggagtgagg tgcatttgca gtcagctttc gctcaccact aagatggatg 661 cagagcatcc ggaactcagg agttacgctc agagccaagg ttggtggacg ggagagggcg 721 agttcaattt ttccgaagtc ttttctccag ttgaggatca tctagactgc ggtgctggca 781 aagacagctt agaaaaacaa gaagaaagca tcacagtgca gactatgatg aacaccttac 841 gggacaaagc cagcggagtg tgcatagact ctgagttttt cctcaccaca gccagtggag 901 tgtctgtcct gccgcagaat agaagctctc cgtgcattca ctacttcact ggaacccatg 961 atccttccag gtccatattc aagcctttca tctttgttga tgacgtaaaa cttgtcccca 1021 aaacacagtc tccctgtttt ggggatgacg accctgccaa aaaggagcct cggttccagg 1081 agaaaccaga ccgccggcat gagctgtaca aagcccacga gtgggcacgt gccatcatcg 1141 aaagtgacca ggagcaaggt cgcaagctga ggagcaccat gctggagctg gagaagcaag 1201 gcctggaagc catggaagaa atcctgacca gctccgagcc actggaccct gcggaagtgg 1261 gggacctttt ctatgactgt gttgacacgg agattaagtt ctttaagtgc ccaactttct 1321 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1381 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta