Construct: ORF ccsbBroad304_07518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011873.1_s304c1, BRDN0000461006
- Derived from:
- ccsbBroadEn_07518
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HS3ST3B1 (9953)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07518
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9953 | HS3ST3B1 | heparan sulfate-glucosamine... | NM_006041.3 | 100% | 100% | |
| 2 | human | 9955 | HS3ST3A1 | heparan sulfate-glucosamine... | NM_006042.3 | 77.3% | 68.3% | (many diffs) |
| 3 | human | 9955 | HS3ST3A1 | heparan sulfate-glucosamine... | XM_011524114.3 | 52.4% | 52.4% | 1_2delATins554;81C>T;619_621delGGA |
| 4 | human | 9953 | HS3ST3B1 | heparan sulfate-glucosamine... | XM_017025479.2 | 52% | 52% | 0_1ins561 |
| 5 | human | 9953 | HS3ST3B1 | heparan sulfate-glucosamine... | NR_130138.2 | 38.1% | 1_438del;1609_3070del | |
| 6 | mouse | 54710 | Hs3st3b1 | heparan sulfate (glucosamin... | NM_018805.2 | 87.4% | 88.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gcagcgcctg agtggcggca gatcttgcct cgatgtcccc ggccggctcc 121 taccgcagcc gccgccgccc ccgccgccgg tgaggaggaa gctcgcgctg ctcttcgcca 181 tgctctgcgt ctggctctat atgttcctgt actcgtgcgc cggctcctgc gccgccgcgc 241 cggggctgct gctcctgggc tctgggtccc gcgccgcaca cgacccgcca gccctggcca 301 cagctccgga cgggacgccc cccaggctgc cgttccgggc gccgccagcc accccactgg 361 cttcaggcaa ggagatggcc gagggcgctg cgagcccgga ggagcagagt cccgaggtgc 421 cggactcccc aagccccatc tccagctttt tcagtgggtc tgggagcaag cagctgccgc 481 aggccatcat catcggcgtg aagaagggcg gcacgcgggc gctgctggag tttctgcgcg 541 tgcaccccga cgtgcgcgcc gtgggcgccg agccccattt cttcgatcgc agctacgaca 601 agggcctcgc ttggtaccgg gacctgatgc ccagaaccct ggacgggcag atcaccatgg 661 agaagacgcc cagttacttc gtcacgcggg aggcccctgc gcgcatctcg gccatgtcca 721 aggacaccaa gctcatcgtg gtggtgcggg acccggtgac cagggccatc tcggactaca 781 cgcagacgct gtccaagcgg cccgacatcc ccaccttcga gagcttgacg ttcaaaaaca 841 ggacagcggg cctcatcgac acgtcgtgga gcgccatcca gatcggcatc tacgccaagc 901 acctggagca ctggctgcgc cacttcccca tccgccagat gctcttcgtg agcggcgagc 961 ggctcatcag cgacccggcc ggggagctgg gccgcgtgca agacttcctg ggcctcaaga 1021 ggatcatcac ggacaagcac ttctacttca acaagaccaa gggcttcccc tgcctgaaga 1081 aggcggaggg cagcagccgg ccccattgcc tgggcaagac caagggcagg acccatcctg 1141 agatcgaccg cgaggtggtg cgcaggctgc gcgagttcta ccggcctttc aacctcaagt 1201 tctaccagat gaccgggcac gactttggct gggattgccc aactttcttg tacaaagtgg 1261 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1321 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta