Transcript: Human NM_006042.3

Homo sapiens heparan sulfate-glucosamine 3-sulfotransferase 3A1 (HS3ST3A1), mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
HS3ST3A1 (9955)
Length:
4186
CDS:
801..2021

Additional Resources:

NCBI RefSeq record:
NM_006042.3
NBCI Gene record:
HS3ST3A1 (9955)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006042.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000035834 CACGTCCCTTTACGTCTTCTA pLKO.1 899 CDS 100% 4.950 6.930 N HS3ST3A1 n/a
2 TRCN0000427687 TAAGCATCAGTTGTCATTAAA pLKO_005 2287 3UTR 100% 15.000 10.500 N HS3ST3A1 n/a
3 TRCN0000035835 CTTCCGGAAGTTCTTGCTGAT pLKO.1 863 CDS 100% 4.050 2.835 N HS3ST3A1 n/a
4 TRCN0000035837 CTCCCTGCTCACGTCCCTTTA pLKO.1 890 CDS 100% 3.600 2.520 N HS3ST3A1 n/a
5 TRCN0000445792 TTCTTGCTGATGCTCTGCTCC pLKO_005 873 CDS 100% 2.160 1.512 N HS3ST3A1 n/a
6 TRCN0000035836 CCTTTACGTCTTCTACTGCCT pLKO.1 905 CDS 100% 0.660 0.462 N HS3ST3A1 n/a
7 TRCN0000098087 CGACTTTGGCTGGGATGGATA pLKO.1 2000 CDS 100% 4.950 2.970 N Hs3st3a1 n/a
8 TRCN0000035817 CAAGCACTTCTACTTCAACAA pLKO.1 1814 CDS 100% 4.950 2.475 Y HS3ST3B1 n/a
9 TRCN0000035816 GCCTTTCAACCTCAAGTTCTA pLKO.1 1964 CDS 100% 4.950 2.475 Y HS3ST3B1 n/a
10 TRCN0000035838 CAACCTCAAGTTCTACCAGAT pLKO.1 1970 CDS 100% 4.050 2.025 Y HS3ST3A1 n/a
11 TRCN0000035818 CAAGAGGATCATCACGGACAA pLKO.1 1796 CDS 100% 4.050 2.025 Y HS3ST3B1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006042.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07519 pDONR223 100% 99.8% 99.5% None 803G>A;891C>N n/a
2 ccsbBroad304_07519 pLX_304 0% 99.8% 99.5% V5 803G>A;891C>N n/a
3 TRCN0000492022 TGCTGAGTCCCGGTAATACGACAT pLX_317 16.3% 99.8% 99.5% V5 803G>A;891C>N n/a
4 ccsbBroadEn_07518 pDONR223 100% 77.3% 68.3% None (many diffs) n/a
5 ccsbBroad304_07518 pLX_304 0% 77.3% 68.3% V5 (many diffs) n/a
6 TRCN0000480208 AGGTAAGGCCCAATTTCTATAAAT pLX_317 29.9% 77.3% 68.3% V5 (many diffs) n/a
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