Construct: ORF ccsbBroad304_07538
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012771.1_s304c1, BRDN0000408595
- Derived from:
- ccsbBroadEn_07538
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GJC1 (10052)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07538
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10052 | GJC1 | gap junction protein gamma 1 | NM_001080383.1 | 99.7% | 99.7% | 15C>T;1175C>A;1179C>T |
| 2 | human | 10052 | GJC1 | gap junction protein gamma 1 | NM_005497.4 | 99.7% | 99.7% | 15C>T;1175C>A;1179C>T |
| 3 | human | 10052 | GJC1 | gap junction protein gamma 1 | XM_005256920.1 | 99.7% | 99.7% | 15C>T;1175C>A;1179C>T |
| 4 | human | 10052 | GJC1 | gap junction protein gamma 1 | XM_024450525.1 | 99.7% | 99.7% | 15C>T;1175C>A;1179C>T |
| 5 | human | 10052 | GJC1 | gap junction protein gamma 1 | XM_024450526.1 | 99.7% | 99.7% | 15C>T;1175C>A;1179C>T |
| 6 | human | 10052 | GJC1 | gap junction protein gamma 1 | XM_024450527.1 | 99.7% | 99.7% | 15C>T;1175C>A;1179C>T |
| 7 | human | 10052 | GJC1 | gap junction protein gamma 1 | XR_934346.3 | 68.3% | (many diffs) | |
| 8 | mouse | 14615 | Gjc1 | gap junction protein, gamma 1 | NM_001159382.1 | 92.2% | 96.9% | (many diffs) |
| 9 | mouse | 14615 | Gjc1 | gap junction protein, gamma 1 | NM_001159383.1 | 92.2% | 96.9% | (many diffs) |
| 10 | mouse | 14615 | Gjc1 | gap junction protein, gamma 1 | NM_008122.3 | 92.2% | 96.9% | (many diffs) |
| 11 | mouse | 14615 | Gjc1 | gap junction protein, gamma 1 | XM_006532226.3 | 92.2% | 96.9% | (many diffs) |
| 12 | mouse | 14615 | Gjc1 | gap junction protein, gamma 1 | XM_006532227.3 | 92.2% | 96.9% | (many diffs) |
| 13 | mouse | 14615 | Gjc1 | gap junction protein, gamma 1 | XM_011248736.2 | 92.2% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1257
- ORF length:
- 1188
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagttggagc tttctgactc gcctgctaga ggagattcac aaccattcca 121 catttgtggg gaagatctgg ctcactgttc tgattgtctt ccggatcgtc cttacagctg 181 taggaggaga atccatctat tacgatgagc aaagcaaatt tgtgtgcaac acagaacagc 241 cgggctgtga gaatgtctgt tatgatgcgt ttgcacctct ctcccatgta cgcttctggg 301 tgttccagat catcctggtg gcaactccct ctgtgatgta cctgggctat gctatccaca 361 agattgccaa aatggagcac ggtgaagcag acaagaaggc agctcggagc aagccctatg 421 caatgcgctg gaaacaacac cgggctctgg aagaaacgga ggaggacaac gaagaggatc 481 ctatgatgta tccagagatg gagttagaaa gtgataagga aaataaagag cagagccaac 541 ccaaacctaa gcatgatggc cgacgacgga ttcgggaaga tgggctcatg aaaatctatg 601 tgctgcagtt gctggcaagg accgtgtttg aggtgggttt tctgataggg cagtattttc 661 tgtatggctt ccaagtccac ccgttttatg tgtgcagcag acttccttgt cctcataaga 721 tagactgctt tatttctaga cccactgaaa agaccatctt ccttctgata atgtatggtg 781 ttacaggcct ttgcctcttg cttaacattt gggagatgct tcatttaggg tttgggacca 841 ttcgagactc actaaacagt aaaaggaggg aacttgagga tccgggtgct tataattatc 901 ctttcacttg gaatacacca tctgctcccc ctggctataa cattgctgtc aaaccagatc 961 aaatccagta caccgaactg tccaatgcta agatcgccta caagcaaaac aaggccaaca 1021 cagcccagga acagcagtat ggcagccatg aggagaacct cccagctgac ctggaggctc 1081 tgcagcggga gatcaggatg gctcaggaac gcttggatct ggcagttcag gcctacagtc 1141 accaaaacaa ccctcatggt ccccgggaga agaaggccaa agtggggtcc aaagctgggt 1201 ccaacaaaag cactgccagt agcaaatcag gggatgggaa gaactctgtc tggattttgc 1261 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1321 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1381 tta