Construct: ORF ccsbBroad304_07609

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003406.1_s304c1, BRDN0000406940
Derived from:
ccsbBroadEn_07609
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LRRN2 (10446)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07609
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10446 LRRN2 leucine rich repeat neuronal 2 NM_006338.3 99.8% 99.7% 1064T>C;1476A>G;2026G>C
2 human 10446 LRRN2 leucine rich repeat neuronal 2 NM_201630.2 99.8% 99.7% 1064T>C;1476A>G;2026G>C
3 human 10446 LRRN2 leucine rich repeat neuronal 2 XM_005244827.3 99.8% 99.7% 1064T>C;1476A>G;2026G>C
4 human 10446 LRRN2 leucine rich repeat neuronal 2 XM_011509066.2 99.8% 99.7% 1064T>C;1476A>G;2026G>C
5 human 10446 LRRN2 leucine rich repeat neuronal 2 XM_017000050.2 99.8% 99.7% 1064T>C;1476A>G;2026G>C
6 mouse 16980 Lrrn2 leucine rich repeat protein... NM_010732.4 85.3% 87.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2205
ORF length:
2139
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag gcttctcgtg gccccactct tgctagcttg ggtggctggt gccactgccg
121ctgtgcccgt ggtaccctgg catgttccct gcccccctca gtgtgcctgc cagatccggc
181cctggtatac gccccgctcg tcctaccgcg aggctaccac tgtggactgc aatgacctat
241tcctgacggc agtccccccg gcactccccg caggcacaca gaccctgctc ctgcagagca
301acagcattgt ccgtgtggac cagagtgagc tgggctacct ggccaatctc acagagctgg
361acctgtccca gaacagcttt tcggatgccc gagactgtga tttccatgcc ctgccccagc
421tgctgagcct gcacctagag gagaaccagc tgacccggct ggaggaccac agctttgcag
481ggctggccag cctacaggaa ctctatctca accacaacca gctctaccgc atcgccccca
541gggccttttc tggcctcagc aacttgctgc ggctgcacct caactccaac ctcctgaggg
601ccattgacag ccgctggttt gaaatgctgc ccaacttgga gatactcatg attggcggca
661acaaggtaga tgccatcctg gacatgaact tccggcccct ggccaacctg cgtagcctgg
721tgctagcagg catgaacctg cgggagatct ccgactatgc cctggagggg ctgcaaagcc
781tggagagcct ctccttctat gacaaccagc tggcccgggt gcccaggcgg gcactggaac
841aggtgcccgg gctcaagttc ctagacctca acaagaaccc gctccagcgg gtagggccgg
901gggactttgc caacatgctg caccttaagg agctgggact gaacaacatg gaggagctgg
961tctccatcga caagtttgcc ctggtgaacc tccccgagct gaccaagctg gacatcacca
1021ataacccacg gctgtccttc atccaccccc gcgccttcca ccacctgccc cagatggaga
1081ccctcatgct caacaacaac gctctcagtg ccttgcacca gcagacggcg gagtccctgc
1141ccaacctgca ggaggtaggt ctccacggca accccatccg ctgtgactgt gtcatccgct
1201gggccaatgc cacgggcacc cgtgtccgct tcatcgagcc gcaatccacc ctgtgtgcgg
1261agcctccgga cctccagcgc ctcccggtcc gtgaggtgcc cttccgggag atgacggacc
1321actgtttgcc cctcatctcc ccacgaagct tccccccaag cctccaggta gccagtggag
1381agagcatggt gctgcattgc cgggcactgg ccgaacccga acccgagatc tactgggtca
1441ctccagctgg gcttcgactg acacctgccc atgcaggcag gaggtaccgg gtgtaccccg
1501aggggaccct ggagctgcgg agggtgacag cagaagaggc ggggctatac acctgtgtgg
1561cccagaacct ggtgggggct gacactaaga cggttagtgt ggttgtgggc cgtgctctcc
1621tccagccagg cagggacgaa ggacaggggc tggagctccg ggtgcaggag acccacccct
1681atcacatcct gctatcttgg gtcaccccac ccaacacagt gtccaccaac ctcacctggt
1741ccagtgcctc ctccctccgg ggccaggggg ccacagctct ggcccgcctg cctcggggaa
1801cccacagcta caacattacc cgcctccttc aggccacgga gtactgggcc tgcctgcaag
1861tggcctttgc tgatgcccac acccagttgg cttgtgtatg ggccaggacc aaagaggcca
1921cttcttgcca cagagcctta ggggaccgtc ctgggctcat tgccatcctg gctctcgctg
1981tccttctcct ggcagctggg ctagcggccc accttggcac aggccaaccc aggaagggtg
2041tgggtgggag gcggcctctc cctccagcct gggctttctg gggctggagt cccccttctg
2101tccgggttgt gtctgctccc ctcgtcctgc cctggaatcc agggaggaag ctgcccagat
2161cctcagaagg ggagacactg ttgccaccat tgtctcaaaa ttcttgccca actttcttgt
2221acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
2281gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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