Construct: ORF ccsbBroad304_07619
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000333.1_s304c1, BRDN0000409039
- Derived from:
- ccsbBroadEn_07619
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC23B (10483)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07619
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10483 | SEC23B | SEC23 homolog B, COPII coat... | NM_001172745.3 | 99.9% | 99.7% | 1117A>G;1298C>T |
| 2 | human | 10483 | SEC23B | SEC23 homolog B, COPII coat... | NM_006363.6 | 99.9% | 99.7% | 1117A>G;1298C>T |
| 3 | human | 10483 | SEC23B | SEC23 homolog B, COPII coat... | NM_032985.6 | 99.9% | 99.7% | 1117A>G;1298C>T |
| 4 | human | 10483 | SEC23B | SEC23 homolog B, COPII coat... | NM_032986.4 | 99.9% | 99.7% | 1117A>G;1298C>T |
| 5 | human | 10483 | SEC23B | SEC23 homolog B, COPII coat... | XM_017027593.1 | 99.9% | 99.7% | 1117A>G;1298C>T |
| 6 | human | 10483 | SEC23B | SEC23 homolog B, COPII coat... | NM_001172746.3 | 97.5% | 97.3% | 364_365ins54;1063A>G;1244C>T |
| 7 | mouse | 27054 | Sec23b | SEC23 homolog B, COPII coat... | NM_001252543.1 | 90.5% | 96.8% | (many diffs) |
| 8 | mouse | 27054 | Sec23b | SEC23 homolog B, COPII coat... | NM_001252544.1 | 90.5% | 96.8% | (many diffs) |
| 9 | mouse | 27054 | Sec23b | SEC23 homolog B, COPII coat... | NM_001252545.1 | 90.5% | 96.8% | (many diffs) |
| 10 | mouse | 27054 | Sec23b | SEC23 homolog B, COPII coat... | NM_019787.4 | 90.5% | 96.8% | (many diffs) |
| 11 | mouse | 27054 | Sec23b | SEC23 homolog B, COPII coat... | XM_017318899.1 | 90.5% | 96.8% | (many diffs) |
| 12 | mouse | 27054 | Sec23b | SEC23 homolog B, COPII coat... | NM_001281816.1 | 72.6% | 77.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2367
- ORF length:
- 2301
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gacatacctg gagttcatcc agcagaatga agaacgggat ggtgtgcgtt 121 ttagttggaa cgtgtggcct tccagccggc tggaggctac aagaatggtt gtacccctgg 181 cttgtctcct tactcctttg aaagaacgtc cagacctacc tcctgtacaa tatgaacctg 241 tgctttgcag caggccaact tgtaaagctg ttctcaaccc actttgtcag gttgattatc 301 gagcaaaact ttgggcctgt aatttctgtt ttcaaagaaa tcagtttcct ccagcttatg 361 gaggcatatc tgaggtgaat caacctgccg aattgatgcc ccagttttct acaattgagt 421 acgtgataca gcgaggtgct cagtcccctc tgatctttct ctatgtggtt gacacatgcc 481 tggaggaaga tgaccttcaa gcactcaaag agtccctgca gatgtccctg agtcttcttc 541 ctccagatgc tctggtgggt ctgatcacat ttggaaggat ggtgcaggtt catgagctaa 601 gctgtgaagg aatctccaaa agttatgtct tccgagggac caaggattta actgcaaagc 661 aaatacagga tatgttgggc ctgaccaagc cagccatgcc catgcagcaa gcacgacctg 721 cacaaccaca ggagcaccct tttgcttcaa gcagatttct gcagcctgtt cacaagattg 781 atatgaacct cactgatctt cttggggagc tacagaggga cccatggcca gtaactcagg 841 ggaagagacc tttgcgatcc actggtgtgg ctttgtccat tgctgttggc ttgctggagg 901 gcacttttcc aaacacagga gccaggatca tgctgtttac tggaggtccc cctacccaag 961 ggcctggcat ggtggttgga gatgaattaa agattcctat tcgttcttgg catgatattg 1021 agaaagataa tgcacgattc atgaaaaagg caaccaagca ctatgagatg cttgctaatc 1081 gaacagctgc aaatggtcac tgcattgata tttatgcttg tgcccttgat caaactggac 1141 ttttggagat gaagtgttgt gcaaatctta ctggaggcta cgtggtaatg ggagattctt 1201 tcaacacttc tctcttcaag cagacattcc aaagaatctt tactaaagat tttaatggag 1261 atttccgaat ggcatttggt gctactttgg acgtaaagac ctctcgggaa ctgaagattg 1321 caggagccat tggtccatgc gtatctctga atgtgaaagg actgtgtgtg tcagaaaatg 1381 agcttggtgt tggtggcacg agtcagtgga aaatctgtgg cctagatcct acatctacac 1441 ttggcatcta ttttgaagtt gtcaatcagc acaacacccc gatcccccaa ggaggcagag 1501 gagccatcca gtttgtcacg cattatcagc actccagcac ccagagacgc atccgcgtga 1561 ccaccatcgc ccgaaattgg gcagatgtac agagtcagct caggcacata gaagcagcat 1621 ttgaccagga ggctgcggca gtgttgatgg cacggcttgg ggtgttccga gcggagtcag 1681 aggaggggcc cgatgtgctc cggtggctgg accgacaact catccgactg tgtcaaaagt 1741 ttggacagta taacaaagaa gaccccactt cttttaggtt atcagattcc ttttctctat 1801 atcctcagtt tatgttccat ctgagaagat ctccatttct tcaagtgttt aacaacagtc 1861 ctgatgagtc gtcatattac agacatcatt ttgcccggca ggacctgacc cagtccctca 1921 tcatgatcca gcccattctc tactcttact cctttcatgg gccaccagag ccagtactct 1981 tggatagcag cagcattcta gctgacagaa ttttgctgat ggatactttc tttcaaattg 2041 tcatttatct tggtgagacc atagcccagt ggcgtaaagc tggctaccag gacatgcccg 2101 agtatgaaaa cttcaagcac cttctgcagg caccactgga tgatgctcaa gaaattctgc 2161 aagcacgctt cccgatgcca cgttacatca acacggagca tggaggcagt caggctcgat 2221 tccttttgtc caaagtgaac ccatctcaga cacacaataa cctgtatgct tggggacagg 2281 aaactggagc acccatccta actgatgatg ttagcctgca ggtgttcatg gaccatttga 2341 agaagctggc tgtctccagt gcctgttacc caactttctt gtacaaagtg gttggtaagc 2401 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 2461 ggcgcgttaa gtcgacaatc aacctctgga tta