Construct: ORF ccsbBroad304_07636
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004889.1_s304c1, BRDN0000398785
- Derived from:
- ccsbBroadEn_07636
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPTLC1 (10558)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07636
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10558 | SPTLC1 | serine palmitoyltransferase... | NM_178324.3 | 99.7% | 99.3% | 418G>C |
| 2 | human | 10558 | SPTLC1 | serine palmitoyltransferase... | XM_011518138.2 | 38.9% | 38.6% | 418G>C;429_1095delinsA |
| 3 | human | 10558 | SPTLC1 | serine palmitoyltransferase... | NM_006415.4 | 30% | 29.8% | 418G>C;429_1419delinsA |
| 4 | human | 10558 | SPTLC1 | serine palmitoyltransferase... | NM_001281303.2 | 27.7% | 27.4% | 418G>C;429_1539delinsA |
| 5 | mouse | 268656 | Sptlc1 | serine palmitoyltransferase... | XM_006516927.1 | 44.5% | 47.2% | (many diffs) |
| 6 | mouse | 268656 | Sptlc1 | serine palmitoyltransferase... | NM_009269.2 | 25.3% | 26.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 495
- ORF length:
- 429
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccgccacg gagcagtggg ttctggtgga gatggtacag gcgctttacg 121 aggctcctgc ttaccatctt attttggaag ggattctgat cctctggata atcagacttc 181 ttttctctaa gacttacaaa ttacaagaac gatctgatct tacagtcaag gaaaaagaag 241 aactgattga agagtggcaa ccagaacctc ttgttcctcc tgtcccaaaa gaccatcctg 301 ctctcaacta caacatcgtt tcaggccctc caagccacaa aactgtggtg aatggaaaag 361 aatgtataaa cttcgcctca tttaattttc ttggattgtt ggataaccct agggttaagg 421 cagcagcttt agcatctcta aagaagtatg gcgtggggac ttgtggaccc agaggatttt 481 atcgcacatt tgaatgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 541 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 601 cgacaatcaa cctctggatt a