Construct: ORF ccsbBroad304_07673
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008096.1_s304c1, BRDN0000400239
- Derived from:
- ccsbBroadEn_07673
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAI2 (10742)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07673
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10742 | RAI2 | retinoic acid induced 2 | NM_001172739.1 | 99.9% | 99.8% | 1024G>C |
| 2 | human | 10742 | RAI2 | retinoic acid induced 2 | NM_001172743.1 | 99.9% | 99.8% | 1024G>C |
| 3 | human | 10742 | RAI2 | retinoic acid induced 2 | NM_021785.5 | 99.9% | 99.8% | 1024G>C |
| 4 | human | 10742 | RAI2 | retinoic acid induced 2 | XM_006724459.2 | 99.8% | 99.6% | 754A>G;1024G>C |
| 5 | human | 10742 | RAI2 | retinoic acid induced 2 | XM_006724460.1 | 99.8% | 99.6% | 754A>G;1024G>C |
| 6 | human | 10742 | RAI2 | retinoic acid induced 2 | XM_011545439.2 | 99.8% | 99.6% | 754A>G;1024G>C |
| 7 | human | 10742 | RAI2 | retinoic acid induced 2 | XM_011545440.2 | 99.8% | 99.6% | 754A>G;1024G>C |
| 8 | human | 10742 | RAI2 | retinoic acid induced 2 | XM_011545441.2 | 99.8% | 99.6% | 754A>G;1024G>C |
| 9 | human | 10742 | RAI2 | retinoic acid induced 2 | NM_001172732.1 | 90.5% | 90.3% | 134_135ins150;874G>C |
| 10 | human | 10742 | RAI2 | retinoic acid induced 2 | NR_033349.1 | 68.8% | 1_343del;1367G>C;1934_2308del | |
| 11 | human | 10742 | RAI2 | retinoic acid induced 2 | NR_033348.1 | 63.5% | 1_537del;1561G>C;2128_2502del | |
| 12 | mouse | 24004 | Rai2 | retinoic acid induced 2 | NM_001103367.1 | 87.6% | 89.6% | (many diffs) |
| 13 | mouse | 24004 | Rai2 | retinoic acid induced 2 | NM_198409.3 | 87.6% | 89.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1656
- ORF length:
- 1590
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgacctgcag tcccagaacc tctccatgga catgactgac tcccctcctg 121 ccttggctaa taacagactg gagaatggca tggctcagct gatcaccacc gaggcctgga 181 acatcaactc cactgacctg gtaaagaagg ccctggtgac cgtgccagcc ccatccattc 241 tgaacccccc tgccgagtct cagagtggca tggctctgaa ggtggcggcc actgtgttgc 301 agcccctgtg cctcggggag agcccagtgg tgatgcccat tcacatgcag gtggagggaa 361 gctccgcacc agagctcaat ccgaatggca atgccaccta cgtcatgacc acccagggcc 421 ccgtgcaact gcccgtggtg ctggagcagc acgtctttca gcacctcaac tcccctctgg 481 tcctgccgca ggaggcccca tgctcctcca gtaccatcca caacaacctc ttccagggag 541 cggaggaccc cgaggcccag ccccagctcc tggacctgag gatccccagc cagccgcagg 601 agcccacttt gccatttgaa gctgtgctcc agaatttgtt tccctcccag ggcactctcg 661 ggcccccacc ctgtcagcct cctcctggct atgcccctgt gcccccacag ccttttagct 721 cccccttgtc ccccctggtc ccaccagcca ccctcttggt gccgtatcct gtaatcgtcc 781 ccttgcctgt gccagtccct attcccatcc ccatcccggt gcctcagagt tctgaatcca 841 agttcagctc cagtttcccc aagccaccat cttccttcgg cctgcacccc tttaaaggca 901 cccagacccc tctggaaaaa gatgaactga agccctttga catcctccag cctaaggagt 961 acttccagct cagccgccac acggtcatta agatgggaag tgagaacgag gccctggatc 1021 tctccatgaa gtcagtgccc tggctcaagg ctggtgaagt cagtccccca atcttccagg 1081 aagatgcacc cctagacctg tcagtggcag cccaccggaa atccgagcct ccccctgaga 1141 cactgtatga cagtggtgca tcagtggaca gctcaggtca cacagtgatg gagaaacttc 1201 ccagtggcat ggaaatttct tttgcccctg ccacgtccca tgaggcccca gccatgatgg 1261 atagtcacat cagcagcagt gatgctgcta ccgagatgct cagccagccc aaccacccca 1321 gcggcgaagt caaggctgaa aataacattg agatggtggg cgagtcccag gcggccaagg 1381 tcattgtctc tgtcgaagat gctgtgccta ccatattctg tggcaagatc aaaggcctct 1441 caggggtgtc caccaaaaac ttctccttca aaagagaaga ctccgtgctt cagggctatg 1501 acatcaacag ccaaggggaa gagtccatgg gaaatgcaga gccccttagg aaacccatca 1561 aaaaccggag cataaagtta aagaaagtga actcccagga aatacacatg ctcccaatca 1621 aaaaacaacg gctggccacc ttttttccaa gaaagtaccc aactttcttg tacaaagtgg 1681 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1741 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta