Construct: ORF ccsbBroad304_07699

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000259.1_s304c1, BRDN0000411386
Derived from:
ccsbBroadEn_07699
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GPR83 (10888)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07699
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10888 GPR83 G protein-coupled receptor 83 NM_016540.4 99.9% 100% 1182T>C
2 human 10888 GPR83 G protein-coupled receptor 83 NM_001330345.2 89.9% 90% 386_387ins126;1056T>C
3 mouse 14608 Gpr83 G protein-coupled receptor 83 NM_010287.3 85.4% 88.9% (many diffs)
4 mouse 14608 Gpr83 G protein-coupled receptor 83 XM_006510017.2 81.6% 84.9% (many diffs)
5 mouse 14608 Gpr83 G protein-coupled receptor 83 NM_001310734.1 76.8% 79.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1335
ORF length:
1269
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt ccctcacctc ttgctgctct gtctcctccc cttggtgcga gccaccgagc
121cccacgaggg ccgggccgac gagcagagcg cggaggcggc cctggccgtg cccaatgcct
181cgcacttctt ctcttggaac aactacacct tctccgactg gcagaacttt gtgggcagga
241ggcgctacgg cgctgagtcc cagaacccca cggtgaaagc cctgctcatt gtggcttact
301ccttcatcat tgtcttctca ctctttggca acgtcctggt ctgtcatgtc atcttcaaga
361accagcgaat gcactcggcc accagcctct tcatcgtcaa cctggcagtt gccgacataa
421tgatcacgct gctcaacacc cccttcactt tggttcgctt tgtgaacagc acatggatat
481ttgggaaggg catgtgccat gtcagccgct ttgcccagta ctgctcactg cacgtctcag
541cactgacact gacagccatt gcggtggatc gccaccaggt catcatgcac cccttgaaac
601cccggatctc aatcacaaag ggtgtcatct acatcgctgt catctggacc atggctacgt
661tcttttcact cccacatgct atctgccaga aattatttac cttcaaatac agtgaggaca
721ttgtgcgctc cctctgcctg ccagacttcc ctgagccagc tgacctcttc tggaagtacc
781tggacttggc caccttcatc ctgctctaca tcctgcccct cctcatcatc tctgtggcct
841acgctcgtgt ggccaagaaa ctgtggctgt gtaatatgat tggcgatgtg accacagagc
901agtactttgc cctgcggcgc aaaaagaaga agaccatcaa gatgttgatg ctggtggtag
961tcctctttgc cctctgctgg ttccccctca actgctacgt cctcctcctg tccagcaagg
1021tcatccgcac caacaatgcc ctctactttg ccttccactg gtttgccatg agcagcacct
1081gctataaccc cttcatatac tgctggctga acgagaactt caggattgag ctaaaggcat
1141tactgagcat gtgtcaaaga cctcccaagc ctcaggagga caggccaccc tccccagttc
1201cttccttcag ggtggcctgg acagagaaga atgatggcca gagggccccc cttgccaata
1261acctcctgcc cacctcccaa ctccagtctg ggaagacaga cctgtcatct gtggaaccca
1321ttgtgacgat gagttaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1381ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1441cgacaatcaa cctctggatt a

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