Construct: ORF ccsbBroad304_07722
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008395.1_s304c1, BRDN0000403737
- Derived from:
- ccsbBroadEn_07722
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF2C (11004)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07722
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11004 | KIF2C | kinesin family member 2C | NM_006845.4 | 99.9% | 99.8% | 2093G>C |
| 2 | human | 11004 | KIF2C | kinesin family member 2C | NM_001297655.2 | 94.2% | 94% | 439_440ins123;1970G>C |
| 3 | human | 11004 | KIF2C | kinesin family member 2C | NM_001297656.2 | 92.4% | 92.2% | 0_1ins163;2delT;1931G>C |
| 4 | human | 11004 | KIF2C | kinesin family member 2C | NM_001297657.2 | 84.3% | 84.2% | 0_1ins339;1754G>C |
| 5 | human | 11004 | KIF2C | kinesin family member 2C | XM_011540540.2 | 84.3% | 84.2% | 0_1ins339;1754G>C |
| 6 | human | 11004 | KIF2C | kinesin family member 2C | XM_011540541.2 | 84.3% | 84.2% | 0_1ins339;1754G>C |
| 7 | mouse | 73804 | Kif2c | kinesin family member 2C | NM_134471.4 | 86.2% | 86.9% | (many diffs) |
| 8 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_006503433.2 | 80.5% | 81.3% | (many diffs) |
| 9 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_011240620.2 | 80.5% | 81.3% | (many diffs) |
| 10 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_011240621.2 | 80.5% | 81.3% | (many diffs) |
| 11 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_017320411.1 | 80.5% | 81.3% | (many diffs) |
| 12 | mouse | 73804 | Kif2c | kinesin family member 2C | NM_001290662.1 | 80.1% | 81.3% | (many diffs) |
| 13 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_006503435.3 | 80.1% | 81.3% | (many diffs) |
| 14 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_011240622.2 | 80.1% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2241
- ORF length:
- 2175
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc catggactcg tcgcttcagg cccgcctgtt tcccggtctc gctatcaaga 121 tccaacgcag taatggttta attcacagtg ccaatgtaag gactgtgaac ttggagaaat 181 cctgtgtttc agtggaatgg gcagaaggag gtgccacaaa gggcaaagag attgattttg 241 atgatgtggc tgcaataaac ccagaactct tacagcttct tcccttacat ccgaaggaca 301 atctgccctt gcaggaaaat gtaacaatcc agaaacaaaa acggagatcc gtcaactcca 361 aaattcctgc tccaaaagaa agtcttcgaa gccgctccac tcgcatgtcc actgtctcag 421 agcttcgcat cacggctcag gagaatgaca tggaggtgga gctgcctgca gctgcaaact 481 cccgcaagca gttttcagtt cctcctgccc ccactaggcc ttcctgccct gcagtggctg 541 aaataccatt gaggatggtc agcgaggaga tggaagagca agtccattcc atccgaggca 601 gctcttctgc aaaccctgtg aactcagttc ggaggaaatc atgtcttgtg aaggaagtgg 661 aaaaaatgaa gaacaagcga gaagagaaga aggcccagaa ctctgaaatg agaatgaaga 721 gagctcagga gtatgacagt agttttccaa actgggaatt tgcccgaatg attaaagaat 781 ttcgggctac tttggaatgt catccactta ctatgactga tcctatcgaa gagcacagaa 841 tatgtgtctg tgttaggaaa cgcccactga ataagcaaga attggccaag aaagaaattg 901 atgtgatttc cattcctagc aagtgtctcc tcttggtaca tgaacccaag ttgaaagtgg 961 acttaacaaa gtatctggag aaccaagcat tctgctttga ctttgcattt gatgaaacag 1021 cttcgaatga agttgtctac aggttcacag caaggccact ggtacagaca atctttgaag 1081 gtggaaaagc aacttgtttt gcatatggcc agacaggaag tggcaagaca catactatgg 1141 gcggagacct ctctgggaaa gcccagaatg catccaaagg gatctatgcc atggcctccc 1201 gggacgtctt cctcctgaag aatcaaccct gctaccggaa gttgggcctg gaagtctatg 1261 tgacattctt cgagatctac aatgggaagc tgtttgacct gctcaacaag aaggccaagc 1321 tgcgcgtgct ggaggacggc aagcaacagg tgcaagtggt ggggctgcag gagcatctgg 1381 ttaactctgc tgatgatgtc atcaagatga tcgacatggg cagcgcctgc agaacctctg 1441 ggcagacatt tgccaactcc aattcctccc gctcccacgc gtgcttccaa attattcttc 1501 gagctaaagg gagaatgcat ggcaagttct ctttggtaga tctggcaggg aatgagcgag 1561 gcgcggacac ttccagtgct gaccggcaga cccgcatgga gggcgcagaa atcaacaaga 1621 gtctcttagc cctgaaggag tgcatcaggg ccctgggaca gaacaaggct cacaccccgt 1681 tccgtgagag caagctgaca caggtgctga gggactcctt cattggggag aactctagga 1741 cttgcatgat tgccacgatc tcaccaggca taagctcctg tgaatatact ttaaacaccc 1801 tgagatatgc agacagggtc aaggagctga gcccccacag tgggcccagt ggagagcagt 1861 tgattcaaat ggaaacagaa gagatggaag cctgctctaa cggggcgctg attccaggca 1921 atttatccaa ggaagaggag gaactgtctt cccagatgtc cagctttaac gaagccatga 1981 ctcagatcag ggagctggag gagaaggcta tggaagagct caaggagatc atacagcaag 2041 gaccagactg gcttgagctc tctgagatga ccgagcagcc agactatgac ctggagacct 2101 ttgtgaacaa agcggaatct gctctggccc agcaagccaa gcatttctca gccctgccag 2161 atgtcatcaa ggccttgcgc ctggccatgc agctggaaga gcaggctagc agacaaataa 2221 gcagcaagaa acggccccag tgcccaactt tcttgtacaa agtggttggt aagcctatcc 2281 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 2341 ttaagtcgac aatcaacctc tggatta