Construct: ORF ccsbBroad304_07746

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015700.1_s304c1, BRDN0000398893
Derived from:
ccsbBroadEn_07746
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WDR5 (11091)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07746
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11091 WDR5 WD repeat domain 5 NM_017588.3 99.9% 100% 606A>G
2 human 11091 WDR5 WD repeat domain 5 NM_052821.3 99.9% 100% 606A>G
3 human 11091 WDR5 WD repeat domain 5 XM_005272163.2 99.9% 100% 606A>G
4 human 11091 WDR5 WD repeat domain 5 XM_024447393.1 99.9% 100% 606A>G
5 human 11091 WDR5 WD repeat domain 5 XM_024447394.1 99.9% 100% 606A>G
6 human 11091 WDR5 WD repeat domain 5 XM_024447395.1 99.9% 100% 606A>G
7 human 54554 WDR5B WD repeat domain 5B NM_019069.4 79.7% 85% (many diffs)
8 human 401127 LOC401127 WD repeat domain 5 pseudogene NR_026854.1 54.5% (many diffs)
9 mouse 140858 Wdr5 WD repeat domain 5 NM_080848.2 87.6% 100% (many diffs)
10 mouse 140858 Wdr5 WD repeat domain 5 XM_006497672.3 87.6% 100% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1068
ORF length:
1002
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gacggaggag aagaagcccg agaccgaggc cgccagagca cagccaaccc
121cttcgtcatc cgccactcag agcaagccta cacctgtgaa gccaaactat gctctaaagt
181tcacccttgc tggccacacc aaagcagtgt cctccgtgaa attcagcccg aatggagagt
241ggctggcaag ttcatctgct gataaactta ttaaaatttg gggcgcgtat gatgggaaat
301ttgagaaaac catatctggt cacaagctgg gaatatccga tgtagcctgg tcgtcagatt
361ctaaccttct tgtttctgcc tcagatgaca aaaccttgaa gatatgggac gtgagctcgg
421gcaagtgtct gaaaaccctg aagggacaca gtaattatgt cttttgctgc aacttcaatc
481cccagtccaa ccttattgtc tcaggatcct ttgacgaaag cgtgaggata tgggatgtga
541aaacagggaa gtgcctcaag actttgccag ctcactcgga tccagtctcg gccgttcatt
601ttaatcgtga tggatccttg atagtttcaa gtagctatga tggtctctgt cgcatctggg
661acaccgcctc gggccagtgc ctgaagacgc tcatcgatga cgacaacccc cccgtgtctt
721ttgtgaagtt ctccccgaac ggcaaataca tcctggccgc cacgctggac aacactctga
781agctctggga ctacagcaag gggaagtgcc tgaagacgta cactggccac aagaatgaga
841aatactgcat atttgccaat ttctctgtta ctggtgggaa gtggattgtg tctggctcag
901aggataacct tgtttacatc tggaaccttc agacgaaaga gattgtacag aaactacaag
961gccacacaga tgtcgtgatc tcaacagctt gtcacccaac agaaaacatc atcgcctctg
1021ctgcgctaga aaatgacaaa acaattaaac tgtggaagag tgactgctac ccaactttct
1081tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1141aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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