Construct: ORF ccsbBroad304_07756

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012827.1_s304c1, BRDN0000401130
Derived from:
ccsbBroadEn_07756
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RCAN3 (11123)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07756
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11123 RCAN3 RCAN family member 3 NM_001251977.2 99.5% 100% 414G>A;462G>A;663G>A
2 human 11123 RCAN3 RCAN family member 3 NM_001251978.1 99.5% 100% 414G>A;462G>A;663G>A
3 human 11123 RCAN3 RCAN family member 3 NM_001251979.2 99.5% 100% 414G>A;462G>A;663G>A
4 human 11123 RCAN3 RCAN family member 3 NM_013441.4 99.5% 100% 414G>A;462G>A;663G>A
5 human 11123 RCAN3 RCAN family member 3 NM_001251980.1 95.4% 95.8% (many diffs)
6 human 11123 RCAN3 RCAN family member 3 NM_001251981.1 75.5% 75.9% (many diffs)
7 human 11123 RCAN3 RCAN family member 3 NM_001251982.1 71.6% 55.1% 366_367ins172;491G>A;519_520ins32
8 human 11123 RCAN3 RCAN family member 3 NM_001251983.1 47.7% 48.1% (many diffs)
9 human 11123 RCAN3 RCAN family member 3 NM_001251984.1 47.7% 48.1% (many diffs)
10 human 11123 RCAN3 RCAN family member 3 NM_001251985.1 47.5% 32.3% 193_194ins346;317G>A;345_346ins32
11 mouse 53902 Rcan3 regulator of calcineurin 3 NM_022980.4 80.4% 89.6% (many diffs)
12 mouse 53902 Rcan3 regulator of calcineurin 3 XM_017320317.1 80.4% 89.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
789
ORF length:
723
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct gagggacact atgaaatctt ggaatgatag ccagtcagat ctgtgtagca
121ctgaccaaga agaggaagaa gagatgattt ttggtgaaaa tgaagatgat ttggatgaga
181tgatggattt aagtgatctg cctacctcac tttttgcttg cagcgtccat gaagcagtgt
241ttgaggcacg agagcagaag gaaagatttg aagcactctt caccatctat gatgaccagg
301ttacttttca gctgtttaaa agctttagaa gagtcagaat aaatttcagc aaacctgaag
361cggcagcaag agcgcgaata gaactccacg aaacagactt caatgggcag aagctaaagc
421tatattttgc acaggtgcag atgtccggcg aagtgcggga caagtcctat ctcctgccac
481cccagcctgt caagcagttc ctcatctccc ctccagcctc tcccccagtg gggtggaagc
541agagcgaaga tgcgatgcct gttataaatt atgatttact ctgtgctgtt tccaaattgg
601gaccaggaga gaaatatgaa cttcacgcgg gaacagagtc gacacccagc gtggtggttc
661atgtctgtga aagtgaaact gaagaggaag aagagacaaa aaaccccaaa cagaaaattg
721cccagacaag gcgccccgac cctccgaccg cagcgttgaa tgagccccag acctttgatt
781gcgcgctgtg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
841tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
901tcaacctctg gatta

Download FASTA (ORF) (Full)