Construct: ORF ccsbBroad304_07814

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012980.1_s304c1, BRDN0000407130
Derived from:
ccsbBroadEn_07814
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SACM1L (22908)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07814
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22908 SACM1L SAC1 like phosphatidylinosi... NM_014016.5 99.9% 99.8% 1301A>T
2 human 22908 SACM1L SAC1 like phosphatidylinosi... NM_001319071.2 96.3% 99.8% 1301A>T;1762_1827del
3 human 22908 SACM1L SAC1 like phosphatidylinosi... NM_001319072.2 89.5% 78.5% 0_1ins55;147_148ins128;1118A>T
4 human 22908 SACM1L SAC1 like phosphatidylinosi... XM_011533500.2 89.5% 78.5% 0_1ins55;147_148ins128;1118A>T
5 human 22908 SACM1L SAC1 like phosphatidylinosi... NM_001319073.2 82.3% 82.2% 0_1ins309;992A>T
6 human 22908 SACM1L SAC1 like phosphatidylinosi... XR_940392.3 45.3% (many diffs)
7 mouse 83493 Sacm1l SAC1 suppressor of actin mu... NM_030692.4 91% 94.8% (many diffs)
8 mouse 83493 Sacm1l SAC1 suppressor of actin mu... XM_017313703.1 81.7% 75.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1827
ORF length:
1761
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gacggcggcc tacgagcagc tgaagctgca tatcacacct gaaaaatttt
121atgtggaagc ttgtgatgat ggagcagatg acgtacttac cattgaccgt gtgtccacag
181aggttaccct tgcagtcaag aaagatgttc ctccttcagc tgtcacaaga ccaatatttg
241gtatactggg cacaatccat ctggtggcag gtaattatct tatagtcatt accaaaaaga
301taaaagtagg tgaatttttc agtcatgtag tctggaaagc aacagatttt gatgtccttt
361cttataagaa gacaatgttg cacttaactg atattcagtt acaagataat aaaaccttcc
421tagcgatgct aaaccatgtc ttgaatgtgg atggatttta cttttcaaca acatatgatt
481tgacccatac tttgcagcgg ctatccaaca ctagtcctga attccaagaa atgagtctct
541tggaaagggc agatcagcgg tttgtatgga atggtcatct tctaagagaa ctttctgcac
601agccagaggt tcatcggttt gcccttccag tgttacatgg ctttattacc atgcattcat
661gttctattaa tggaaaatac tttgattgga ttctcatctc gaggaggagc tgtttcagag
721ctggtgtgcg ctattatgta agaggaattg attcggaagg ccatgcagct aactttgtag
781aaacagaaca aattgtgcac tacaatggga gcaaagcttc gtttgtacag actcgaggat
841caatacctgt tttctggtcc caaagaccaa acctcaagta caaaccactg ccacagatca
901gcaaagtagc aaatcacatg gacggtttcc aaaggcattt tgattcccaa gtaattattt
961atggaaaaca agttataatc aatctgatta accagaaggg ctcggagaag ccacttgagc
1021agacatttgc aacaatggtg tcttccttgg gaagtggaat gatgagatac attgcctttg
1081acttccataa ggaatgtaaa aatatgagat gggatcgact aagtatttta ttggatcagg
1141tagcagaaat gcaagatgaa ttaagttatt ttctagtgga ctctgctggc caggtggtgg
1201caaaccagga aggcgtgttc cgaagcaatt gcatggattg tctagataga accaatgtga
1261tccagagttt gttagctcgt cgttcacttc aggcccaact tcagagacta ggagttttgc
1321atgtgggaca aaagcttgaa gaacaagatg aatttgagaa gattttcaaa aatgcctggg
1381ctgacaacgc aaatgcttgt gccaagcaat atgcgggaac tggtgccttg aagactgact
1441ttaccagaac tggaaagaga actcatttgg gacttataat ggatggctgg aactcaatga
1501tacgatatta taagaacaac ttttccgatg gatttagaca agattccata gacttatttc
1561ttggaaacta ttcagtggat gaattagaat ctcatagtcc tttaagtgtt ccaagggact
1621ggaaattcct ggctttgcct attatcatgg ttgttgcctt ttcaatgtgc attatctgtt
1681tgcttatggc tggtgacact tggacagaaa cactggccta tgtgctcttc tggggagttg
1741caagcattgg aacatttttt atcattcttt acaatggcaa agattttgtc gatgctccca
1801gactggtcca gaaagaaaag atagactgcc caactttctt gtacaaagtg gttggtaagc
1861ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
1921ggcgcgttaa gtcgacaatc aacctctgga tta

Download FASTA (ORF) (Full)