Construct: ORF ccsbBroad304_07825
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016205.1_s304c1, BRDN0000462064
- Derived from:
- ccsbBroadEn_07825
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SV2C (22987)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07825
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22987 | SV2C | synaptic vesicle glycoprote... | NM_014979.4 | 99.9% | 99.8% | 360G>A;1037T>C |
| 2 | human | 22987 | SV2C | synaptic vesicle glycoprote... | XM_011543281.3 | 99.9% | 99.8% | 360G>A;1037T>C |
| 3 | human | 22987 | SV2C | synaptic vesicle glycoprote... | XM_011543282.3 | 99.9% | 99.8% | 360G>A;1037T>C |
| 4 | human | 22987 | SV2C | synaptic vesicle glycoprote... | NM_001297716.2 | 92.1% | 90.9% | (many diffs) |
| 5 | human | 22987 | SV2C | synaptic vesicle glycoprote... | XM_017009244.2 | 51.1% | 48.2% | (many diffs) |
| 6 | mouse | 75209 | Sv2c | synaptic vesicle glycoprote... | NM_029210.1 | 88.8% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2250
- ORF length:
- 2181
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagactct tacaaggata ggacttcact gatgaagggt gccaaggaca 121 ttgccagaga ggtgaagaaa caaacagtaa agaaggtgaa tcaagctgtg gaccgagccc 181 aggatgaata cacccagagg tcctacagtc ggttccaaga tgaagaagat gatgatgact 241 actacccggc tggagaaacc tataatggtg aggccaacga tgacgaaggc tcaagtgaag 301 ccactgaggg gcatgatgaa gatgatgaga tctatgaggg ggagtatcag ggcatcccca 361 gtatgaacca agcgaaggac agcatcgtgt cagtggggca gcccaagggc gatgagtaca 421 aggaccgacg ggagctggaa tcagaaagga gagctgacga ggaagagtta gcccagcagt 481 atgagctgat aatccaagaa tgcggtcatg gtcgttttca gtgggccctt ttcttcgtcc 541 tgggcatggc tcttatggca gacggtgtag aggtgtttgt cgttggcttc gtgttaccca 601 gtgctgagac agacctctgc atcccaaatt caggatctgg atggctaggc agcatagtgt 661 acctcgggat gatggtgggg gcgttcttct ggggaggact ggcagacaaa gtgggaagga 721 aacagtctct tctgatttgc atgtctgtca acggattctt tgccttcctt tcttcatttg 781 tccaaggtta tggcttcttt ctcttctgtc gcttactttc tggattcggg attggaggag 841 ccatacccac tgtgttctcg tactttgctg aagtcctggc ccgggaaaag cggggcgaac 901 acttgagctg gctctgcatg ttctggatga tcggtggcat ctacgcctct gccatggcct 961 gggccatcat cccgcactac gggtggagct tcagcatggg atcggcctac cagtttcaca 1021 gttggcgtgt gtttgtcatc gtctgtgcac tcccctgtgt ctcctccgtg gtggccctca 1081 cattcatgcc tgaaagccca cgatccttgt tggaggttgg aaaacatgat gaagcttgga 1141 tgattctgaa gttaattcat gacaccaaca tgagagcccg gggtcagcct gagaaggtct 1201 tcacggtaaa caaaataaaa actcctaaac aaatagatga gctgattgaa attgagagtg 1261 acacaggaac atggtatagg aggtgttttg ttcggatccg caccgagctg tacggaattt 1321 ggttgacttt tatgagatgt ttcaactacc cagtcaggga taatacaata aagcttacaa 1381 ttgtttggtt caccctgtcc tttgggtact atggattatc cgtttggttc cctgatgtca 1441 ttaaacctct gcagtccgat gaatatgcat tgctaaccag aaatgtggag agagataaat 1501 atgcaaattt cactattaac tttacaatgg aaaatcagat tcatactgga atggaatacg 1561 acaatggcag attcataggg gtcaagttca aatctgtaac tttcaaagac tctgttttta 1621 agtcctgcac ctttgaggat gtaacttcag tgaacaccta cttcaagaac tgcacattta 1681 ttgacactgt ttttgacaac acagattttg agccatataa attcattgac agtgaattta 1741 aaaactgctc gttttttcac aacaagacgg gatgtcagat tacctttgat gatgactata 1801 gtgcctactg gatttatttt gtcaactttc tggggacatt ggcagtattg ccagggaaca 1861 ttgtgtctgc tctgctgatg gacagaattg ggcgcttaac aatgctaggt ggctctatgg 1921 tgctttcggg gatcagctgt ttcttccttt ggttcggcac cagtgaatcc atgatgatag 1981 gcatgctgtg tctgtacaat ggattgacca tctcagcctg gaactctctt gacgtggtca 2041 ctgtggaact gtaccccaca gaccggaggg caacaggctt tggcttctta aatgcgctat 2101 gcaaggcagc agccgtcctg ggaaacttaa tatttggctc tctggtcagc atcaccaaat 2161 caatccccat cctgctggct tctactgtgc tcgtgtgtgg aggactcgtt gggctgtgcc 2221 tgcctgacac acgaacccag gttctgatgt tgccaacttt cttgtacaaa gtggttggta 2281 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2341 ggtggcgcgt taagtcgaca atcaacctct ggatta