Construct: ORF ccsbBroad304_07826

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013504.1_s304c1, BRDN0000461297
Derived from:
ccsbBroadEn_07826
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KLHDC10 (23008)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07826
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23008 KLHDC10 kelch domain containing 10 NM_014997.4 99.9% 99.7% 302A>G
2 human 23008 KLHDC10 kelch domain containing 10 XM_005250233.5 93.3% 93.2% 164_165ins87;215A>G
3 mouse 76788 Klhdc10 kelch domain containing 10 NM_029742.3 90.4% 96.3% (many diffs)
4 mouse 76788 Klhdc10 kelch domain containing 10 NM_001311087.1 84% 89.8% (many diffs)
5 mouse 76788 Klhdc10 kelch domain containing 10 XM_017321829.1 81.2% 81.5% (many diffs)
6 mouse 76788 Klhdc10 kelch domain containing 10 XM_030255666.1 59.2% 54.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1392
ORF length:
1326
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc ggccgcccag ggctgggaca ggaaccgccg gaggggagga ggcgccgccg
121gcgctggtgg cggaggtagc ggggccggcg ggggcagtgg gggcagcggg ggtcggggga
181ctggccagct caaccgcttc gtgcaactct ccgggcggcc gcacctgcca ggtaagaaga
241aaatacgatg ggacccagtt aggaggcgct tcattcagtc ctgtcccatc ataaggatcc
301ctaacaggtt tttgagaggc cacagacctc caccagcacg aagtggacat cgttgtgtgg
361cagatagtac caacctatat gtgtttggag gttataaccc agattatgat gaatcgggag
421ggcctgataa tgaagactat cctctcttca gggaactctg gaggtatcat tttgctacag
481gagtatggca ccagatgggc acagatggct acatgccccg ggaattggca tctatgtcac
541ttgtgctgca tggaaacaac ctgttagtat ttggaggtac gggcatccca tttggagaga
601gcaacggcaa tgacgtccat gtgtgtaatg tgaagtataa gagatgggct ttgctcagct
661gtcgggggaa gaaacccagt cgtatatatg gacaggctat ggccatcatc aatggctccc
721tttatgtctt tggaggtaca accggctata tttacagcac agacctgcac aagttagatc
781tcaataccag agagtggaca caactgaaac caaacaacct atcctgtgat ctaccagaag
841agagataccg acatgaaatt gcacatgacg ggcagaggat ttacatcttg ggaggtggta
901cttcctggac agcatattcc ttaaacaaga tccatgcata caaccttgaa acgaatgcct
961gggaggaaat tgcaacaaaa ccccatgaaa aaataggctt tcctgcagcc cgaaggtgtc
1021acagttgtgt tcaaataaaa aatgatgtat ttatttgtgg gggctataat ggagaggtga
1081tcctgggaga tatctggaag ttgaatctgc agactttcca atgggtgaag ctcccagcta
1141ccatgccaga gccagtttat tttcactgtg cagctgttac accagctggt tgcatgtaca
1201ttcatggagg agtggtgaac atccatgaaa acaaacggac tgggtcattg tttaagatct
1261ggctggtggt acctagcctg ctggaactgg catgggagaa gctgcttgcg gccttcccta
1321accttgcaaa cctctcccga acacaacttc tgcaccttgg actcacacag ggactcatcg
1381aacgcttgaa atgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1441tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1501caatcaacct ctggatta

Download FASTA (ORF) (Full)